We narrowed to 29,740 results for: REP

1. pACEC3GH (Apr)

   Plasmid

   #110085

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. N-BLa 1.1

   Plasmid

   #88994

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the N-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   N-BLa 1.1 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a-baPrs-hdNadV

   Plasmid

   #91950

   Purpose

   pET28a-baPrs-hdNadV is a bicistronic vector designed for the simultaneous expression of PRS from Bacillus amyloliquefaciens and NadV from Haemophilus ducreyi

   Depositor

   George Cătălin Marinescu

   Article

   Marinescu et al Sci Rep. 2018 Aug 16;8(1):12278. doi: 10

   Inserts

   Putative Nicotinamide Phosphoribosyl Transferase (nadV Haemophilus ducreyi, strain: ATCC 27722)
   Phosphoribosyl Pyrophosphate Synthetases
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   changed Leucine 135 to Isoleucine

   Promoter

   T7

   Available Since

   Sept. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1(-) + SLNCR1

   Plasmid

   #86816

   Purpose

   mammalian expression of lncRNA LINC00673/SLNCR1

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   LINC00673 (LINC00673 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-EF1a-double floxed-Aldh1a1-HA-WPRE-HGHpA

   Plasmid

   #184633

   Purpose

   Expresses Aldh1a1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Aldh1a1 (Aldh1a1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG.Myc-SARS2_3CL

   Plasmid

   #200120

   Purpose

   Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

   Depositor

   Eiji Morita

   Article

   Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

   Insert

   SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   May 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. C-BLa 1.1

   Plasmid

   #88997

   Purpose

   BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.1 fusionprotein containing the C-terminal fragment of the split β-lactamase.

   Depositor

   Dieter Langosch

   Article

   Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103

   Insert

   C-BLa 1.1 fusion protein
   

   Use

   Tags

   Flag

   Expression

   Bacterial

   Mutation

   Promoter

   araBAD

   Available Since

   May 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. 1000.pCCLsin.cPPT.hPGK.Brn2.MIRT124_x4 Xma_lost.WPRE

   Plasmid

   #67295

   Purpose

   Induce human iN conversion from fibroblast

   Depositor

   Malin Parmar

   Article

   Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

   Insert

   Brn2 (Pou3f2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   Aug. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. GOMO-GFP-LUC

   Plasmid

   #60976

   Purpose

   Rapid selection of recombinant marker-free bioluminescent rodent malaria parasites

   Depositor

   Olivier Silvie

   Article

   Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

   Inserts

   GFP-Luciferase fusion
   mCherry
   hDHFR-yFCU
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   April 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJet-CMV-CD5Flag2-bglpA

    Plasmid

    #89705

    Purpose

    Expression plasmid for Flag embedded into human CD52 with short polyA

    Depositor

    Dmitriy Mazurov

    Article

    Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

    Insert

    CMV-CD5Flag2-bglpA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAGGS-Flex-GFP

    Plasmid

    #197883

    Purpose

    Can be used to generate AAV virus that will express GFP in the presence of Cre

    Depositor

    Kazuto Kobayashi

    Article

    Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    N/A

    Promoter

    CAG

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAC-mCherry2-G4

    Plasmid

    #164167

    Purpose

    Expresses mCherry2 under the control of CMV promoter, basic vector for AAV, pAAV-CMV backbone, multiclonig site at C terminal

    Depositor

    Isei Tanida

    Article

    Tanida et al Sci Rep. 2020 Dec 14;10(1):21871. doi: 1

    Insert

    mCherry2
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRSET-TEV-12His

    Plasmid

    #137032

    Purpose

    empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Type

    Empty backbone

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE

    Plasmid

    #67292

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Myt1L (Myt1l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pD765 FDFT1 3'UTR

    Plasmid

    #105765

    Purpose

    Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

    Depositor

    Joseph Ziegelbauer

    Article

    Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

    Insert

    FDFT1 3'UTR (FDFT1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    None

    Promoter

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSicoR-shS6K1/2

    Plasmid

    #81088

    Purpose

    expresses an shRNA targeting both S6K1 and S6K2 (mouse)

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    S6K1 & S6K2 shRNA
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. epiCRISPRn

    Plasmid

    #135961

    Purpose

    Expresses SpCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HS-DN-Ect2

    Plasmid

    #105956

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

    Insert

    Ect2 (ECT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pGEX4T-1 Srap1

    Plasmid

    #89691

    Purpose

    Expresses murine Srap1 in bacteria

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Murine SRAP domain ortholog Srap1 (Hmces Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE

    Plasmid

    #67293

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Myt1L (Myt1l Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian and Yeast

    Mutation

    Promoter

    PGK

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. aL-mEos3.2

    Plasmid

    #113154

    Purpose

    constrained LFA-1-mEos3.2 fusion

    Depositor

    Timothy Springer

    Article

    Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

    Insert

    full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)
    

    Use

    Tags

    mEOS3.2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSico-shRheb

    Plasmid

    #81087

    Purpose

    conditional expression of an shRNA targeting murine Rheb

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    Rheb shRNA (Rheb Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. 3xFN-Tel-TAL/pCMV-7.1

    Plasmid

    #63589

    Purpose

    Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.

    Depositor

    Hodaka Fujii

    Article

    Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

    Insert

    3xFVN-Tel-TAL
    

    Use

    TALEN

    Tags

    3xFLAG-tag, V5-tag, and nuclear localization sign…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCDH_ss-SBP-EGFP

    Plasmid

    #172358

    Purpose

    Reporter to monitoring a streptavidin-binding peptide trafficking

    Depositor

    Guido Kroemer

    Article

    Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

    Insert

    ER importing signal sequence
    

    Use

    Lentiviral

    Tags

    SBP-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pACYCDuet_D27NDHFR_WTTS

    Plasmid

    #91244

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    D27N

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pACYCDuet_F31YG121VDHFR_WTTS

    Plasmid

    #91229

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    F31YG121V

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pACYCDuet_G121VDHFR_WTTS

    Plasmid

    #91233

    Purpose

    bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

    Depositor

    Kimberly Reynolds

    Article

    Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

    Inserts

    folA
    thyA
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    G121V

    Promoter

    T7

    Available Since

    June 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pET30a-SRAP-delCys2

    Plasmid

    #84568

    Purpose

    Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Srap1 (Hmces Mouse)
    

    Use

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Mutation

    deletion of Cys2

    Promoter

    T7

    Available Since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDEST-EGFP-MACROD1

    Plasmid

    #172584

    Purpose

    For transient expression of N-terminal GFP-tagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3.1-R2ACR_142-EYFP

    Plasmid

    #103964

    Purpose

    Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

    Insert

    Anion channelrhodopsin R2ACR_142
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    human codon optimized

    Promoter

    CMV

    Available Since

    Nov. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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