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Plasmid#117881Purposeprotein expressionDepositorInsertCHL1 P492L (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationP429LPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHL1 T101D
Plasmid#117879Purposeprotein expressionDepositorInsertCHL1 T101D (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationT101DPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHL1 T101A
Plasmid#117877Purposeprotein expressionDepositorInsertCHL1 T101A (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationT101APromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHL1 P492L-eGFP
Plasmid#117882Purposeprotein expressionDepositorInsertCHL1 (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationP492LPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRARE-MBP-DEST
Plasmid#84650PurposeGateway cloning vector expresses MBP-tagged proteins under the control of the IPTG– inducible ptac promoter and also expresses several tRNAs that are rare in E. coli.DepositorTypeEmpty backboneUseTagsMBPExpressionBacterialMutationPromotertacAvailable sinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-GFI1
Plasmid#16168DepositorInsertGrowth factor independent 1 (GFI1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 18, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBW2656_pCAG-PV1-nMag-L1-CTD-ENTRY-BGHpA
Plasmid#108849PurposeInsert protein fragment into KpnI/EcoRI to fuse to nMag tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsnMagExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2655_pCAG-PV1-NTD-ENTRY-L1-pMag-NLS-BGHpA
Plasmid#108848PurposeInsert protein fragment into MluI/BspEI to fuse to pMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMag,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2653_pCAG-PV1-NTD-ENTRY-L1-nMag-NLS-BGHpA
Plasmid#108846PurposeInsert protein fragment into MluI/BspEI to fuse to nMag/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsnMag,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2067_pCAG-PV1-GAI-L1-CTD-ENTRY-BGHpA
Plasmid#108845PurposeInsert protein fragment into KpnI/EcoRI to fuse to GAI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAIExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2065_pCAG-PV1-GID1-L1-CTD-ENTRY-BGHpA
Plasmid#108843PurposeInsert protein fragment into KpnI/EcoRI to fuse to GID1 tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1ExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1309_pCAG-PV1-FKBP-L1-CTD-ENTRY-BGHpA
Plasmid#108837PurposeInsert protein fragment into KpnI/EcoRI to fuse to FKBP tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBPExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1312_pCAG-PV1-NTD-ENTRY-L1-ABI-NLS-BGHpA
Plasmid#108840PurposeInsert protein fragment into MluI/BspEI to fuse to ABI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1313_pCAG-PV1-PYL-L1-CTD-ENTRY-BGHpA
Plasmid#108841PurposeInsert protein fragment into KpnI/EcoRI to fuse to PYL tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYLExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2654_pCAG-PV1-pMag-L1-CTD-ENTRY-BGHpA
Plasmid#108847PurposeInsert protein fragment into KpnI/EcoRI to fuse to pMag tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagspMagExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1308_pCAG-PV1-NTD-ENTRY-L1-FRB-NLS-BGHpA
Plasmid#108836PurposeInsert protein fragment into MluI/BspEI to fuse to FRB/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2064_pCAG-PV1-NTD-ENTRY-L1-GAI-NLS-BGHpA
Plasmid#108842PurposeInsert protein fragment into MluI/BspEI to fuse to GAI/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGAI,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1310_pCAG-PV1-NTD-ENTRY-L1-PYL-NLS-BGHpA
Plasmid#108838PurposeInsert protein fragment into MluI/BspEI to fuse to PYL/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsPYL,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1311_pCAG-PV1-ABI-L1-CTD-ENTRY-BGHpA
Plasmid#108839PurposeInsert protein fragment into KpnI/EcoRI to fuse to ABI tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsABIExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1049_pCAG-PV1-NTD-ENTRY-L1-FKBP-NLS-BGHpA
Plasmid#108834PurposeInsert protein fragment into MluI/BspEI to fuse to FKBP/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFKBP,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW1050_pCAG-PV1-NLS-FRB-L1-CTD-ENTRY-BGHpA
Plasmid#108835PurposeInsert protein fragment into KpnI/EcoRI to fuse to FRB/NLS tag on N-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsFRB,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBW2066_pCAG-PV1-NTD-ENTRY-L1-GID1-NLS-BGHpA
Plasmid#108844PurposeInsert protein fragment into MluI/BspEI to fuse to GID1/NLS tags on C-terminusDepositorTypeEmpty backboneUseSynthetic BiologyTagsGID1,NLSExpressionMammalianMutationPromoterAvailable sinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
CHL1 wt-eGFP
Plasmid#117876Purposeprotein expressionDepositorInsertCHL1 wt-eGFP (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationPromoterAOXAvailable sinceDec. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
CHL1 T101D-eGFP
Plasmid#117880Purposeprotein expressionDepositorInsertCHL1T101D-eGFP (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationT101DPromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pIRESN2 H2B-GFP-uvr8at(2x)
Plasmid#44970DepositorUseTagsinternal GFPExpressionMammalianMutationtandem repeat of UVR8 (both have Methionine 1 del…PromoterpCMV IEAvailable sinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-F138I
Plasmid#100922PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-F138I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-LS-L255I
Plasmid#100964PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-LS-L255I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationL255IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-F138I
Plasmid#100965PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-GS-F138I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationF138IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDRF'- AmTryoshka1;3-GS-L255I
Plasmid#100966PurposeYeast expression of fluorescent sensor reporting the activity of ammonium transceptors. Contains a stable reference LSSmOrange nested within the reporting superfolder cpEGFP.DepositorInsertAmTryoshka1;3-GS-L255I (AMT1;3 Mustard Weed)
UseTagsExpressionYeastMutationL255IPromoterPMAAvailable sinceOct. 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
CHL1 T101A-eGFP
Plasmid#117878Purposeprotein expressionDepositorInsertCHL1 T101A-eGFP (AT5G40090 Mustard Weed)
UseTagsExpressionYeastMutationT101APromoterAOXAvailable sinceDec. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-NES
Plasmid#118939PurposeUsed to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NLS
Plasmid#118938PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NES
Plasmid#118940PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CRY2clust-mCherry-RANK
Plasmid#208612PurposeMammalian expression of CRY2clust-mCherry-RANK (Opto-RANKc)DepositorInsertCRY2clust-mCherry-RANK (Cry2 Mustard Weed, Mouse)
UseTagsmCherryExpressionMammalianMutationPromoterCAGAvailable sinceJan. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTara:500*
Plasmid#107954PurposeArabinose-inducible T7*RNAPDepositorInsertT7RNAP (modified)
UseSynthetic BiologyTagsExpressionBacterialMutationR632SPromoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSara*
Plasmid#107955PurposeArabinose-inducible split T7*RNAPDepositorInsertT7RNAP (modified)
UseSynthetic BiologyTagsExpressionBacterialMutationR632SPromoterAvailable sinceApril 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTSaraSZ
Plasmid#107956PurposeArabinose-inducible split T7RNAP with SynZIP domains 17/18DepositorInsertT7RNAP (modified)
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:: pRPS5a-PE2max-NC::tHSP
Plasmid#227694PurposeGolden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.DepositorInsertPE2max-NC being driven by pRPS5a and terminated by tHSP
UseCRISPRTagsExpressionPlantMutationPromoterpRPS5aAvailable sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFP.R267
Plasmid#138179PurposeExpresses the split mCherry reporter interrupted by the gp41-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R270
Plasmid#138219PurposeExpresses the split mCherry reporter interrupted by the IMPDH-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMarch 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R269
Plasmid#138181PurposeExpresses the split mCherry reporter interrupted by the NrdJ-1 intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFP.R376
Plasmid#138246PurposeExpresses the split mCherry reporter interrupted by the Pfu-RIR1-1 artificially mini-intein (in cis)DepositorInsertsUseSynthetic BiologyTags6xHisExpressionBacterialMutationPromoterP(araBAD) and iPCAvailable sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-SOMA
Plasmid#118935PurposeUsed to express the SOMA FRET sensor in E. coli.DepositorInsertSOMA FRET sensor for measuring MAPK activity in Arabidopsis thaliana
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO1(wt)
Plasmid#191798PurposeFull length wt AtPCO1 with Nt-His tagDepositorInsertAtPCO1
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO2(wt)
Plasmid#191799PurposeFull length wt AtPCO2 with Nt-His tagDepositorInsertAtPCO2
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-28a(+)-His(6)-AtPCO3(wt)
Plasmid#191800PurposeFull length wt AtPCO3 with Nt-His tagDepositorInsertAtPCO3
UseTags6xHis, Thrombin cleavage siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-IP3KA
Plasmid#32529DepositorInsertIP3KA (ITPKA Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-AtSCT (JBEI-11557)
Plasmid#87937PurposeCo-expression in S.cerevisiae of 4CL5 and AtSCTDepositorInsertsUseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONRG_P4-P1R:MUM4_0.3Pro_35S
Plasmid#128558PurposeGateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing a 307 bp MUM4 (At1g53500) promoter fragment fused to the 54 bp 35S minimal promoter sequence, for use in Three-way Gateway cloningDepositorTypeEmpty backboneUseGateway promoter entry cloneTagsExpressionMutationPromoterMUM4 core promoter with 35S enhancerAvailable sinceSept. 25, 2019AvailabilityIndustry, Academic Institutions, and Nonprofits -
pPK-351
Plasmid#157921PurposepcDNA-CMV-PIF3MTAD-IRES-PhyBGal4DBDDepositorInsertsPIF3-MTAD
IRES
PhyB(1-621)-SV40NLS-Gal4DBD
UseSynthetic Biology; OptogeneticsTagsHA tag and SV40NLSExpressionMammalianMutationpif3 1-523PromoterCMVAvailable sinceSept. 24, 2020AvailabilityAcademic Institutions and Nonprofits only