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pSLQ1651-sgTelomere(F+E)

Plasmid

#51024

Purpose

Lentiviral vector that contains an optimized S. pyogenes sgRNA targeting human telomeres

Depositor

Bo Huang, Stanley Qi

Article

Chen et al Cell. 2013 Dec 19;155(7):1479-91. doi: 1

Insert

Optimized sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mouse U6 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pCas9D10A_GFP

Plasmid

#44720

Purpose

Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells

Depositor

Kiran Musunuru

Article

Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.

Insert

Cas9D10A-2A-GFP

Use

CRISPR

Tags

2A-GFP

Expression

Mammalian

Mutation

D10A mutation- nickase mutant

Promoter

CAG

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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pBabe puro HA PIK3CA

Plasmid

#12522

Purpose

Retroviral expression of HA tagged phosphoinositide 3-kinase

Depositor

Jean Zhao

Article

Zhao et al Proc Natl Acad Sci U S A. 2005 Dec 20. 1

Insert

PIK3CA (PIK3CA Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Available Since

Aug. 18, 2006

Availability

Academic Institutions and Nonprofits only

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HA-FOXO3a WT

Plasmid

#1787

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FOXO3a (FOXO3 Human)

Tags

HA (YDVPDYASL)

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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HA Raptor

Plasmid

#8513

Depositor

David Sabatini

Article

Kim et al Cell 2002 Jul 26;110(2):163-75.

Insert

Raptor (RPTOR Human)

Tags

ha

Expression

Mammalian

Available Since

April 4, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti PGK V5-LUC Neo (w623-2)

Plasmid

#21471

Purpose

3rd gen lentiviral V5-Luciferase expression vector, PGK promoter, Neo

Depositor

Eric Campeau

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

V5-LUC

Use

Lentiviral and Luciferase

Tags

V5

Expression

Mammalian

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only

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pSLCAR-CD19-28z

Plasmid

#135991

Purpose

Modular CD28-CD3z CAR backbone, For Transient Expression or Lentiviral Production

Depositor

Scott McComb

Article

Bloemberg et al Mol Ther Methods Clin Dev. 2020 Jan 31;1

Insert

FMC63-28-3z-P2A-EGFP (CD28, CD247 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a-short

Available Since

June 10, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Clathrin light chain

Plasmid

#70217

Purpose

Expresses GFP11-clathrin light chain in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP11-Clathrin light chain (CLTA Human)

Tags

GFP11

Expression

Mammalian

Available Since

March 21, 2016

Availability

Academic Institutions and Nonprofits only

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pPAGFP-N1

Plasmid

#11909

Depositor

Jennifer Lippincott-Schwartz

Article

Patterson et al Science. 2002 Sep 13. 297(5588):1873-7.

Has Service

Cloning Grade DNA

Insert

pPAGFP-N1

Expression

Mammalian

Mutation

These plasmids are essentially the same as the Cl…

Available Since

July 3, 2008

Availability

Academic Institutions and Nonprofits only

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pEP4 E02S EN2K

Plasmid

#20925

Purpose

used in the derivation of human iPS cells using non-integrating episomal vectors; expresses Oct4 and Sox2; Nanog and Klf4

Depositor

James Thomson

Article

Yu et al Science. 2009 Mar 26.

Insert

Oct4 and Sox2; Nanog and Klf4 (SOX2, POU5F1, NANOG, KLF4 Human)

Use

Episomal/ebna

Available Since

May 20, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Blast empty (w263-1)

Plasmid

#17486

Purpose

3rd gen lentiviral empty control vector, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-GFP1-10

Plasmid

#80409

Purpose

Expresses GFP1-10 in mammalian cells

Depositor

Bo Huang

Article

Kamiyama et al Nat Commun. 2016 Mar 18;7:11046. doi: 10

Insert

GFP1-10

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Dec. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-PEmax-P2A-GFP

Plasmid

#180020

Purpose

Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

Depositor

David Liu

Article

Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

Insert

PEmax-P2A-EGFP

Tags

P2A-EGFP, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Mutation

Detailed in manuscript

Promoter

CMV

Available Since

Jan. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pC1-HyPer-Red

Plasmid

#48249

Purpose

Genetically encoded hydrogen peroxide indicator with red fluorescence.

Depositor

Vsevolod Belousov

Article

Ermakova et al Nat Commun. 2014 Oct 21;5:5222. doi: 10.

Insert

HYPER-RED

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV G-CEPIA1er

Plasmid

#58215

Purpose

Green fluorescent indicator for calcium imaging in the endoplasmic reticulum

Depositor

Masamitsu Iino

Article

Suzuki et al Nat Commun. 2014 Jun 13;5:4153. doi: 10.

Insert

G-CEPIA1er

Tags

myc

Expression

Mammalian

Mutation

G-GECO1.1 E299D M304L D328N N345K F360W G369S E37…

Promoter

CMV

Available Since

July 18, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-Neo-Bam p53 wt

Plasmid

#16434

Depositor

Bert Vogelstein

Article

Baker et al Science. 1990 Aug 24. 249(4971):912-5.

Insert

p53 (TP53 Human)

Expression

Mammalian

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-hMYC

Plasmid

#20723

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Cell Stem Cell. 2008 Sep 11. 3(3):346-53

Insert

c-MYC (MYC Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 13, 2009

Availability

Academic Institutions and Nonprofits only

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hBax C3-EGFP

Plasmid

#19741

Depositor

Richard Youle

Article

Nechushtan et al EMBO J. 1999 May 4. 18(9):2330-41.

Insert

Bax (BAX Human)

Tags

GFP

Expression

Mammalian

Mutation

none

Available Since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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4xM67 pTATA TK-Luc

Plasmid

#8688

Depositor

Jim Darnell

Article

Besser et al Mol Cell Biol 1999 Feb;19(2):1401-9.

Insert

4 repeated M67 sites

Use

Luciferase

Expression

Mammalian

Available Since

July 18, 2006

Availability

Academic Institutions and Nonprofits only

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pMA122 - peel-1 negative selection

Plasmid

#34873

Purpose

Negative selection marker for use with the Mos-1 mediated system of creating targeted transgene insertions and targeted deletions in C. elegans

Depositor

Erik Jorgensen

Article

Frokjaer-Jensen et al Nat Methods. 2012 Jan 30;9(2):117-8. doi

Insert

peel-1 (peel-1 Nematode)

Use

Worm targeting

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only

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