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We narrowed to 70,667 results for: LAS

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pEntry_SARS-CoV-1-nsp12

Plasmid

#168861

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp12 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD54(R159E)

Plasmid

#172453

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag R159E mutant

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

changed Arginine 159 to Glutamic Acid

Promoter

CMV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pKS022 - pCAGGS-3XFLAG-mKate2-SA2-bpA

Plasmid

#156441

Purpose

For transient expression of mouse SA2 (STAG2 or SCC3B) tagged with mKate2

Depositor

Elphege Nora

Article

Nora et al Nat Commun. 2020 Nov 5;11(1):5612. doi:

Insert

mouse SA2 (Stag2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

none

Promoter

Available since

Sept. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pMT-ubb-Avi-Cerulean-Rpl10a

Plasmid

#79882

Purpose

Ubiquitin promoter driving Avi-tagged protein containing Cerulean protein fused to zebrafish Rpl10a ribosomal unit (Tryon et al. 2012); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Cerulean protein fused to zebrafish Rpl10a ribosomal unit (rpl10a Zebrafish)

Use

Tags

Avi

Expression

Bacterial

Mutation

Promoter

Ubiquitin

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-NL63-Nucleocapsid

Plasmid

#168926

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Nucleocapsid (HCNV63gp6 )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pSNR52-sgTET

Plasmid

#46923

Purpose

Yeast CEN/ARS vector (Ura3) that contains sgRNA controlled by SNR 52 promoter, targeting endogenous TRE elements of pTET07 promoter

Depositor

Jonathan Weissman, Stanley Qi

Article

Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

Insert

sgRNA targeting endogenous TRE elements of pTET07 promoter

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

SNR52

Available since

Oct. 1, 2013

Availability

Academic Institutions and Nonprofits only

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pDONR221 Cas9-RFC5

Plasmid

#183200

Purpose

Entry clone

Depositor

Emma Haapaniemi

Article

Reint et al Elife. 2021 Dec 13;10. pii: 75415. doi:

Insert

Cas9-RFC5

Use

CRISPR; Gateway entry clone

Tags

Expression

Mutation

n/a

Promoter

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pMT-myl7-cytoBirA-2A-mCherry_Ras

Plasmid

#80060

Purpose

Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein

Use

Zebrafish biotagging

Tags

3x HA

Expression

Bacterial

Mutation

Promoter

Myl7 promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGEM-cytoBirA-2A-mCherry-SV40pA-FKF

Plasmid

#79887

Purpose

Donor cassette containing HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), mCherry protein and SV40 polyA tail, followed by FRT site-flanked Kanamycin selection gene

Depositor

Tatjana Sauka-Spengler

Article

Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

Insert

HA-tagged BirA, 2A, mCherry protein and SV40 polyA, followed by FRT site-flanked Kanamycin selection gene

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_SLC2A2

Plasmid

#45267

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

SLC2A2 (SLC2A2 Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2013

Availability

Academic Institutions and Nonprofits only
VP16-ER beta (short)

Plasmid

#11353

Depositor

Donald McDonnell

Article

Chang et al Mol Cell Biol. 1999 Dec . 19(12):8226-39

Insert

ER beta short (ESR2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 10, 2006

Availability

Academic Institutions and Nonprofits only
7432 Bicistronic_GFP_ires_puro

Plasmid

#64336

Purpose

This a retroviral expression plasmid expressing GFP along with puro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Insert

GFP

Use

Retroviral

Tags

GFP

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pDONR223 HCoV-NL63 E_stop

Plasmid

#168684

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR223 HCoV-NL63 E_nostop

Plasmid

#168666

Purpose

Gateway-compatible Entry vector

Depositor

Fritz Roth

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

E (HCNV63gp4 )

Use

Other

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pEntry_HCoV-NL63-Envelope

Plasmid

#168924

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-NL63-Envelope (HCNV63gp4 )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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DICER KO vector

Plasmid

#16656

Depositor

Bert Vogelstein

Article

Cummins et al Proc Natl Acad Sci U S A. 2006 Mar 7. 10

Insert

Dicer knock-out (DICER1 Human)

Use

Adeno-associated viral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2008

Availability

Academic Institutions and Nonprofits only
pSLQ7780 mU6:- || CMV: mCherry~P2A-DD-AcrIIA4

Plasmid

#123658

Purpose

DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control without sgRNA following mU6 promoter.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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SLC9A3R1_PDZ_2

Plasmid

#103933

Purpose

Protein expression and purification of SLC9A3R1 PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SLC9A3R2 (NHERF2 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp8

Plasmid

#168957

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp8 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-HKU1-nsp8

Plasmid

#168897

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-HKU1-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_SARS-CoV-1-nsp8

Plasmid

#168850

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

SARS-CoV-1-nsp8 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_MERS-CoV-nsp8

Plasmid

#168821

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

MERS-CoV-nsp8 (orf1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_1

Plasmid

#103919

Purpose

Protein expression and purification of PTN13 PDZ1 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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PTPN13_PDZ_3

Plasmid

#103921

Purpose

Protein expression and purification of PTN13 PDZ3 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

PTPN13 (PTPN13 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pOTTC1619 pAAV SYN1 Nuc-EYFP miR-30 SST

Plasmid

#135565

Purpose

An AAV vector expressing miR-30a shRNA vs rat SOM (aka SST) and a nuclear EYFP reporter

Depositor

Christopher Richie

Article

Venniro et al Proc Natl Acad Sci U S A. 2020 Apr 7;117

Inserts

miR-30 rat SST
Nuc-EYFP

Use

AAV

Tags

3xNLS

Expression

Mammalian

Mutation

Promoter

SYN1 and hSYN1

Available since

Sept. 8, 2021

Availability

Academic Institutions and Nonprofits only

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7749 Bicistronic_ires_hygro

Plasmid

#64374

Purpose

This a retroviral expression plasmid with a minimal polylinker followed by IRES and hygro resistance gene

Depositor

Georgios Stathopoulos

Article

Agalioti et al Nat Commun. 2017 May 16;8:15205. doi: 10

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

July 3, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2_3X Flag

Plasmid

#194161

Purpose

Expresses eGFP fluorescent protein with a 3X flag tag

Depositor

Stefan Stamm

Article

Welden et al Nucleic Acids Res. 2022 Dec 9;50(22):129

Insert

GFP with 3X Flag

Use

Tags

3x Flag

Expression

Mammalian

Mutation

Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pEntry_HCoV-229E-nsp5

Plasmid

#168954

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-229E-nsp5 (HCoV229Egp1 )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pEntry_HCoV-OC43-nsp5

Plasmid

#168933

Purpose

Gateway-compatible Entry vector

Depositor

Pascal Falter-Braun

Article

Weller et al G3 (Bethesda). 2023 Jul 5;13(7):jkad105.

Insert

HCoV-OC43-nsp5 (ORF1ab )

Use

Gateway-compatible entry vector

Tags

Expression

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-Pcdh15_mini_V8-CD2

Plasmid

#199187

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

mmPcdh15_mini_V8-CD2

Use

AAV

Tags

Expression

Mutation

EC5, EC6, EC8, EC9, EC10 are deleted from msPCDH1…

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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