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We narrowed to 9,944 results for: Gnas

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Showing: 5951 - 6000 of 9944 results

  3. pcDNA3.1_mGFP-FGFR3-K508M

    Plasmid
    #191753
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K508M leads to inactive receptor (target mutation: c.1523A>T; silent mutation added on purpose: c.1515A>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1523A>T (p.K508M) + silent mutation c.1515A&…
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191753-plasmid-map-sequence-id-382274

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  8. pCEP4-HA-Flag-natT1R2 ECD-MHC

    Plasmid
    #113957
    Purpose
    mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R2 (TAS1R2 Human)
    Tags
    FLAG, Signal/leader sequence from influenza hemag…
    Expression
    Mammalian
    Mutation
    residues 22-568 fused to a transmembrane tether
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113957-plasmid-map-sequence-id-215285

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  9. mChF-AIP(TA)

    Plasmid
    #61528
    Purpose
    Expression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TA mutation)
    Depositor
    Takanari Inoue
    Article
    Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:
    Insert
    FK506 binding protein 1A (FKBP1A Human)
    Tags
    AIP with TA mutation (see comments) and mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    April 17, 2015
    Availability
    Academic Institutions and Nonprofits only

  16. pDipA-TEV-His12

    Plasmid
    #137042
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi DipA with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66790.1 (BB_0418 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length protein, contains signal p…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137042-plasmid-map-sequence-id-275698

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  21. pcDNA3.1_mGFP-FGFR3-K650Q

    Plasmid
    #191756
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650Q mutation (associated with HCH; target mutation: c.1948A>C; silent mutation added on purpose: c.1938C>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1948A>C (p.K650Q) + silent mutation c.1938C&…
    Available Since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191756-plasmid-map-sequence-id-382285

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  23. pCEP4-HLA-c-myc-optiT1R3 ECD

    Plasmid
    #113960
    Purpose
    mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain
    Depositor
    Erik Procko
    Article
    Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061
    Insert
    T1R3 (TAS1R3 Human)
    Tags
    Signal/leader sequence from HLA class I histocomp…
    Expression
    Mammalian
    Mutation
    residues 21-563
    Promoter
    cmv
    Available Since
    Feb. 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    113960-plasmid-map-sequence-id-215290

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  35. Alpha MHC-DN.JNK2

    Plasmid
    #51931
    Purpose
    mammalian expression of dominant negative JNK2
    Depositor
    Jeffery Molkentin
    Article
    Liang et al EMBO J. 2003 Oct 1;22(19):5079-89.
    Insert
    DN JNK2 (Mapk9 Mouse)
    Tags
    HA
    Expression
    Mammalian
    Mutation
    dominant negative: the dual phosphorylation moti…
    Promoter
    cardiac-specific α-myosin heavy chain 5.5 kb pro…
    Available Since
    June 17, 2014
    Availability
    Academic Institutions and Nonprofits only

  36. pAN19-3xFLAG-ccvTIR1-NOSt

    Plasmid
    #108546
    Purpose
    Cloning vector including N-terminally 3xFLAG-tagged Arabidopsis auxin receptor TIR1 with the F79G mutation [ccvTIR1] and NOS terminator
    Depositor
    Keiko Torii
    Article
    Uchida et al Nat Chem Biol. 2018 Mar;14(3):299-305. d
    Insert
    TRANSPORT INHIBITOR RESPONSE 1 [F79G] fused with 3xFLAG and NOS terminator (TIR1 Mustard Weed)
    Use
    Cloning vector
    Tags
    3xFLAG
    Mutation
    Changed F79 to G
    Available Since
    May 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    108546-plasmid-map-sequence-id-210054

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  40. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid
    #100282
    Purpose
    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells
    Depositor
    Kazuhiro Aoki
    Article
    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p
    Insert
    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    Tags
    mCherry fused with HRas C-terminus for plasma mem…
    Expression
    Mammalian
    Mutation
    Human codon optimized PhyB (1-621 aa)
    Promoter
    CAG promoter
    Available Since
    Oct. 25, 2017
    Availability
    Academic Institutions and Nonprofits only
    100282-plasmid-map-sequence-id-197258

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  46. pcDNA5-EGFP-NLS-T2A-mCherry-PTS1

    Plasmid
    #87827
    Purpose
    Mammalian expression vector template for co-expression of EGFP-tagged and mCherry-tagged proteins using T2A. NLS and PTS1 can be replaced by proteins of interest.
    Depositor
    Andrea Musacchio
    Article
    Pan et al Elife. 2017 Jan 6;6. pii: e23352. doi: 1
    Inserts
    NLS
    PTS1
    Tags
    EGFP and mCherry
    Expression
    Mammalian
    Promoter
    CMV promoter, tetracycline operator
    Available Since
    April 3, 2017
    Availability
    Academic Institutions and Nonprofits only
    87827-plasmid-map-sequence-id-175008

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  47. pBBA57-TEV-His12

    Plasmid
    #137033
    Purpose
    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12
    Depositor
    Debra Hansen
    Article
    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10
    Insert
    AAC66270.1 (BB_A57 Borrelia burgdorferi B31)
    Tags
    His12 (TEV protease cleavable)
    Expression
    Bacterial
    Mutation
    wild type, full-length BBA57, contains signal pep…
    Promoter
    T7
    Available Since
    March 23, 2020
    Availability
    Academic Institutions and Nonprofits only
    137033-plasmid-map-sequence-id-275688

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  48. mEos3.2FP

    Plasmid
    #203757
    Purpose
    Encodes the membrane anchor of NRas, including the last 10 residues of the C terminus with 1 farnesylation and 1 palmitoylation, with mEos3.2 fused to the N terminus. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    NRas c-term (NRAS Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    203757-plasmid-map-sequence-id-403337

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Showing: 5951 - 6000 of 9944 results