We narrowed to 30,195 results for: REP

Showing: 3601 - 3630 of 30195 results

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pCAG.Myc-SARS2_3CL

Plasmid

#200120

Purpose

Expresses N-terminal Myc-tagged human codon optimyzed SARS-CoV-2 3CL in mammalian cells

Depositor

Eiji Morita

Article

Arakawa et al Sci Rep. 2023 Jan 31;13(1):1753. doi: 10

Insert

SARS-CoV-2 3CL protease (ORF1ab SARS-CoV-2)

Tags

Myc

Expression

Mammalian

Promoter

CAG

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pZac2.1 GfaABC1D ChR2(H134R) mCherry SV40

Plasmid

#112496

Purpose

Expresses channel rhodopsin in astrocytes

Depositor

Baljit Khakh

Article

Octeau et al Cell Rep. 2019 May 21;27(8):2249-2261.e7

Insert

ChR2(H134R)

Use

AAV

Tags

mCherry

Mutation

H134R

Promoter

GfaABC1D

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRSET-TEV-12His

Plasmid

#137032

Purpose

empty vector; C-terminal TEV-protease-cleavable His12; T7 promoter; BseRI cloning sites (e.g., In-Fusion compatible)

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Type

Empty backbone

Tags

His12 (TEV protease cleavable)

Expression

Bacterial

Promoter

T7

Available Since

March 23, 2020

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Myt1L.WPRE

Plasmid

#67292

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Myt1L (Myt1l Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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sgLacZs

Plasmid

#171185

Purpose

Expresses sgRNA

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgLacZ1-hU6-sgLacZ2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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epiCRISPRn

Plasmid

#135961

Purpose

Expresses SpCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Xie et al Sci Rep. 2017 May 24;7(1):2320. doi: 10.

Type

Empty backbone

Expression

Mammalian

Available Since

April 30, 2020

Availability

Academic Institutions and Nonprofits only

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HS-DN-Ect2

Plasmid

#105956

Purpose

heat shock inducible transgenesis

Depositor

Caren Norden

Article

Dzafic et al Cell Rep. 2015 Oct 6;13(1):168-182. doi:

Insert

Ect2 (ECT2 Human)

Tags

EGFP

Expression

Bacterial

Available Since

Feb. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pSicoR-shS6K1/2

Plasmid

#81088

Purpose

expresses an shRNA targeting both S6K1 and S6K2 (mouse)

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

S6K1 & S6K2 shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pD765 FDFT1 3'UTR

Plasmid

#105765

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

FDFT1 3'UTR (FDFT1 Human)

Use

Luciferase

Mutation

None

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX4T-1 Srap1

Plasmid

#89691

Purpose

Expresses murine Srap1 in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Murine SRAP domain ortholog Srap1 (Hmces Mouse)

Tags

GST

Expression

Bacterial

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Myt1L.MIRT124_x4 Xma_lost.WPRE

Plasmid

#67293

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Myt1L (Myt1l Mouse)

Use

Lentiviral

Expression

Mammalian and Yeast

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK.MyoD.WPRE

Plasmid

#122053

Purpose

Lentiviral expression of MyoD

Depositor

Stephen Tapscott

Article

Fong et al Cell Rep. 2015 Mar 31;10(12):1937-46. do

Insert

MyoD (Myod1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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aL-mEos3.2

Plasmid

#113154

Purpose

constrained LFA-1-mEos3.2 fusion

Depositor

Timothy Springer

Article

Moore et al Cell Rep. 2018 Feb 13;22(7):1903-1912. d

Insert

full-length human aL with mEOS3.2 in betapropeller (ITGAL Human)

Tags

mEOS3.2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2018

Availability

Academic Institutions and Nonprofits only

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pSico-shRheb

Plasmid

#81087

Purpose

conditional expression of an shRNA targeting murine Rheb

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

Rheb shRNA (Rheb Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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3xFN-Tel-TAL/pCMV-7.1

Plasmid

#63589

Purpose

Expresses a 3xFLAG-tagged TAL protein recognizing a telomere repeat.

Depositor

Hodaka Fujii

Article

Fujita et al Sci Rep. 2013 Nov 8;3:3171. doi: 10.1038

Insert

3xFVN-Tel-TAL

Use

TALEN

Tags

3xFLAG-tag, V5-tag, and nuclear localization sign…

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCDH_ss-SBP-EGFP

Plasmid

#172358

Purpose

Reporter to monitoring a streptavidin-binding peptide trafficking

Depositor

Guido Kroemer

Article

Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

Insert

ER importing signal sequence

Use

Lentiviral

Tags

SBP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_D27NDHFR_WTTS

Plasmid

#91244

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

D27N

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_F31YG121VDHFR_WTTS

Plasmid

#91229

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

F31YG121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pACYCDuet_G121VDHFR_WTTS

Plasmid

#91233

Purpose

bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Depositor

Kimberly Reynolds

Article

Schober et al Cell Rep. 2019 Jun 11;27(11):3359-3370.e

Inserts

folA
thyA

Tags

6xHis

Expression

Bacterial

Mutation

G121V

Promoter

T7

Available Since

June 18, 2019

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP-delCys2

Plasmid

#84568

Purpose

Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

Srap1 (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Mutation

deletion of Cys2

Promoter

T7

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-R2ACR_142-EYFP

Plasmid

#103964

Purpose

Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells

Depositor

John Spudich

Article

Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.103

Insert

Anion channelrhodopsin R2ACR_142

Tags

EYFP

Expression

Mammalian

Mutation

human codon optimized

Promoter

CMV

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pSN22

Plasmid

#100107

Purpose

expresses a chimeric SOX2-activator fusion

Depositor

Mark Ptashne

Article

Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

Insert

mCitrine-P2A-SOX2 fused to a 'strong' VP16 domain (SOX2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Sept. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER.retro.puro-shMePCE_shLARP7

Plasmid

#113538

Purpose

Retroviral vector designed to knock down MePCE and LARP7 simultaneously.

Depositor

Blerta Xhemalce

Article

Shelton et al Cell Rep. 2018 Feb 6;22(6):1374-1383. do

Insert

shMePCE and shLARP7 (MEPCE Human)

Use

Retroviral

Available Since

July 26, 2018

Availability

Academic Institutions and Nonprofits only

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pJet-CMV-CD5HA2-bglpA

Plasmid

#96962

Purpose

Expression plasmid for HA-tag embedded into human CD52 with short polyA

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

CMV-CD5HA2-bglpA

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-MACROD1

Plasmid

#172584

Purpose

For transient expression of N-terminal GFP-tagged MACROD1

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD1 (MACROD1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-EGFP-MACROD2

Plasmid

#172585

Purpose

For transient expression of N-terminal GFP-tagged MACROD2

Depositor

Karla Feijs

Article

Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

Insert

MACROD2 (MACROD2 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pGK:HygroR-CMV:mKO2-C_UTR

Plasmid

#68481

Purpose

Lentivirus expressing mKO2 with UTR sensor cassette and hygromycin resistance

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Inserts

Hygromycin Resistance
mKO2-C_UTR

Use

Lentiviral

Expression

Mammalian

Mutation

shRNA sensor cassette "B" added to 3&#x…

Promoter

CMV and pGK

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pET30a-SRAP

Plasmid

#84524

Purpose

Expresses murine SRAP domain (250 aa) in bacteria

Depositor

Douglas Feldman

Article

Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

Insert

SRAP domain (Hmces Mouse)

Tags

6xHis and S-Tag

Expression

Bacterial

Promoter

T7

Available Since

Nov. 18, 2016

Availability

Academic Institutions and Nonprofits only

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pD765 HMGCR 3'UTR

Plasmid

#105766

Purpose

Encodes the 3'UTR of relevant gene for validation of miRNAs targeting the 3'UTR in a dual luciferase assay

Depositor

Joseph Ziegelbauer

Article

Ramalingam et al Sci Rep. 2017 Jan 19;7:40813. doi: 10.10

Insert

HMGCR 3'UTR (HMGCR Human)

Use

Luciferase

Mutation

None

Available Since

Feb. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCytERM-EBFP2

Plasmid

#184939

Purpose

ER Visualization (OSER assay) Expresses EBFP2 in fusion with N-terminal part of p450

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

CytERM-EBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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