159,333 results

1. pBad-EBFP2

   Plasmid

   #14891

   Purpose

   Bacterial expression of EBFP2 blue fluorescent protein

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 Apr 20. ():.

   Has Service

   Cloning Grade DNA

   Insert

   EBFP2
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   The fluorescent protein was inserted between XhoI…

   Promoter

   Available since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLS-SceI

   Plasmid

   #137725

   Purpose

   Lentivirus reporter assay plasmid that contains a I-SceI site and a EGFP reporter gene.

   Depositor

   Nadav Ahituv

   Article

   Gordon et al Nat Protoc. 2020 Aug;15(8):2387-2412. do

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ki67-mNeonGreen-IRESpuro2

   Plasmid

   #183737

   Purpose

   Expresses Ki67-mNeonGreen

   Depositor

   Daniel Gerlich

   Article

   Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

   Insert

   MKI67 (MKI67 Human)
   

   Use

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA-HDAC6-FLAG

   Plasmid

   #30482

   Depositor

   Tso-Pang Yao

   Article

   Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

   Insert

   HDAC6 (HDAC6 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. pT7-CalfluxVTN

   Plasmid

   #83926

   Purpose

   Bioluminescent Reporter of Intracellular Calcium

   Depositor

   Carl Johnson

   Article

   Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

   Insert

   CalfluxVTN
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   None

   Promoter

   T7

   Available since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMB80 (R26-CreER)

   Plasmid

   #12168

   Depositor

   Tyler Jacks

   Article

   McLaughlin et al Proc Natl Acad Sci U S A. 2007 Feb 27. 1

   Insert

   Rosa26 CreER^T2
   

   Use

   Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-BRCA1-mCherry

   Plasmid

   #195275

   Purpose

   express mCherry-BRCA1 fusion

   Depositor

   Xiaokun Shu

   Article

   Li et al Sci Adv. 2023 Mar;9(9):eade3760. doi: 10

   Insert

   mCherry-BRCA1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti6/V5-p53_wt p53

   Plasmid

   #22945

   Purpose

   3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag

   Depositor

   Bernard Futscher

   Article

   Junk et al Neoplasia. 2008 May . 10(5):450-61.

   Insert

   tumor protein 53 (TP53 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 27, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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9. MSCV-opEBNA2-IRES-GFP

   Plasmid

   #220354

   Purpose

   Expresses EBV latent gene EBNA2 in mammalian cells (EBNA2 coding sequence was optimised for expression and carries a C-terminal 3xHA-tag)

   Depositor

   Klaus Rajewsky

   Article

   Zhang et al Front Immunol. 2023 Dec 19;14:1331730. d

   Insert

   opEBNA2 (EBNA-2 Epstein-Barr Virus (EBV) strain B95-8)
   

   Use

   Retroviral

   Tags

   3 x HA tag

   Expression

   Mammalian

   Mutation

   codon-optimised for expression in mammalian cells

   Promoter

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE6c

    Plasmid

    #207853

    Purpose

    Mammalian expression of PE6c prime editor

    Depositor

    David Liu

    Article

    Doman et al Cell. 2023 Aug 31;186(18):3983-4002.e26.

    Insert

    PE6c
    

    Use

    Tags

    SV40 bpNLS and SV40 bpNLS, c-Myc NLS

    Expression

    Mammalian

    Mutation

    Tf1rt(P70T, G72V, S87G, M102I, K106R, K118R, I128…

    Promoter

    Available since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. TRE-mCherry-p2A-bReaches-TS

    Plasmid

    #158701

    Purpose

    Reporter construct

    Depositor

    Alice Ting

    Article

    Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

    Insert

    mCherry-p2A-bReaches-TS
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    TRE

    Available since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Ef1a_Large T-antigen_Ires_Puro

    Plasmid

    #18922

    Depositor

    Linzhao Cheng

    Article

    Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

    Insert

    Large T Antigen (SV40gp6 Simian virus 40)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 4, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLenti-CMV-Blast-PIP-FUCCI

    Plasmid

    #138715

    Purpose

    Lentiviral expression of the cell cycle marker PIP-FUCCI

    Depositor

    Jean Cook

    Article

    Grant et al Cell Cycle. 2018;17(21-22):2496-2516. do

    Insert

    PIP-FUCCI
    

    Use

    Lentiviral

    Tags

    mCherry and mVenus

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PEmax-P2A-hMLH1dn

    Plasmid

    #174828

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-human MLH1dn (codon optimized)

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-hMLH1dn
    

    Use

    Tags

    SV40 bpNLS and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript; deletion of residues 754-…

    Promoter

    CMV

    Available since

    Oct. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAVS1-MAFB-IRF8-CEBPA

    Plasmid

    #190720

    Purpose

    For microglia differentiation from iPSC

    Depositor

    Li Gan

    Article

    Drager et al Nat Neurosci. 2022 Aug 11. pii: 10.1038/

    Insert

    MAFB-IRF8-CEBPA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. SLF063: Lenti-fCAG-jRGECO1a

    Plasmid

    #124775

    Purpose

    For general lentiviral or direct expression of cytosolic jRGECO1a

    Depositor

    Adam Cohen

    Article

    Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

    Insert

    jRGECO1a
    

    Use

    Lentiviral

    Tags

    NES-6xHIS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

17. pCDH-kz-CD20-Puro

    Plasmid

    #209759

    Purpose

    Lentiviral transfer plasmid to express the CDS of the human CD20 gene, MS4A1.

    Depositor

    Andrei Thomas-Tikhonenko

    Article

    Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

    Insert

    MS4A1 (MS4A1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3 APEX2-NES

    Plasmid

    #49386

    Purpose

    Expresses APEX2 in the cytosol of mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Flag - APEX2 - NES
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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19. pM-mTagBFP2

    Plasmid

    #228615

    Purpose

    Mammalian expression of mTagBFP2 and extra insert, for MuSIC barcode construction

    Depositor

    Marc Birtwistle

    Article

    Lu et al bioRxiv [Preprint]. 2024 Oct 24:2024.10.

    Insert

    mTagBFP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    We introduced some silent mutations to avoid esse…

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRDA_478

    Plasmid

    #179096

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (SpG)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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