We narrowed to 7,680 results for: CCH
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Plasmid#163971PurposeTrifunctional Cas9-VPR fusion protein plasmid for simultaneous transcriptional activation, transcriptional repression, and genome editing in yeastDepositorInsertCas9-VPR
UseSynthetic BiologyExpressionYeastAvailable SinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2804
Plasmid#63644PurposepCfB2804 is a vector for multiple integrations at sites sharing homology with Ty2Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pHES873
Plasmid#87945PurposeExpresses estradiol-inducible synthetic transcription factor in yeast under control of GAL1 promoter. It activates transcription of promoters containing Zif268 binding sites.DepositorInsertpGAL1- Zif268 DBD - hPR LBD - MSN2 AD (PGR Budding Yeast, Human)
ExpressionYeastPromoterGAL1Available SinceDec. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCMV-CB1R-V2tail-TevN-BLITz1-TetR-VP16
Plasmid#89872Purposeexpresses CB1R-iTango component in mammalian cellsDepositorInsertCB1R-V2tail-TevN-BLITz1-TetR-VP16
ExpressionMammalianPromoterCMVAvailable SinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-link-GFPIvy-SpHis5
Plasmid#60783PurposeYeast C-terminal tagging vector, which creates gene fusions with GFPIvy using SpHis5 selectionDepositorInsertGFPIvy
UseYeast integratingMutationS30R Y39N T65C Q69A S72A F99S N105T Y145F M153T V…Available SinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
BAS287 C terminal E2C hygBR
Plasmid#74082PurposeS.pombe recoded E2Crimson in universal C-terminal tagging plasmid, hygB resistance.DepositorInsertE2Crimson
Available SinceAug. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_Puro
Plasmid#167887PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMXs_FLAG-ODC1
Plasmid#133256PurposeExpress FLAG-tagged ODC1 in mammalian cellsDepositorInsertODC1 (ODC1 Budding Yeast)
UseRetroviralTags3xFLAGExpressionMammalianMutationcodon-optimized for expression in human cellsAvailable SinceSept. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pG4-E6s-luc
Plasmid#46324DepositorInsert5xGal4
UseLuciferaseTagsE boxes and luciferaseExpressionMammalianMutationfive copies of a multimerized GAL4 (5×GAL4) site …Available SinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
HsSPHK2
Plasmid#118092PurposeExpresses sphingosine kinase 2, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)DepositorAvailable SinceAug. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vps4(101-437)
Plasmid#87735PurposeBacterial expression of N-terminally truncated S. cerevisiae Vps4DepositorInsertVacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
Tags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-100PromoterT7Available SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCI-FLAG-mTUT4(D1028A)
Plasmid#60220PurposeMammalian expression of FLAG-tagged catalytic mutant of TUT4DepositorAvailable SinceDec. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRS-Dhh1-PA(D195A,E196A,S226A,T228A)(405)
Plasmid#122067PurposeExpressing Dhh1-PADepositorInsertDhh1-PA (DHH1 Budding Yeast)
TagsPAExpressionYeastMutationAspartic acid 195 to alanine; Glutamic acid 196 t…Available SinceApril 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHCas9-Nours
Plasmid#107733PurposeEscherichia coli- S. cerevisiae shuttle plasmid harbors a Cas9 gene, a natMX6 and URA3 selection markers used in S. cerevisiaeDepositorInsertCas9
UseCRISPRTagsSV40 NLSExpressionYeastMutationHuman optimizedPromoterTEF1Available SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pUAST mito roGFP2-Orp1
Plasmid#64997Purposefor expression of mitochondrial roGFP2-Orp1 with the Gal4 expression system in drosophilaDepositorInsertOrp1 (HYR1 Budding Yeast)
TagsroGFP2ExpressionInsectMutationmitochondrial targeting sequenceAvailable SinceJune 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET-HJURP-CBP
Plasmid#30393DepositorAvailable SinceJuly 11, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGST-TEV-yVms1(1-417,delta(38-69))
Plasmid#128739PurposeBacterial overexpression and crystallizationDepositorInsertVms1 (VCP/Cdc48-associated mitochondria stress-responsive 1) (VMS1 Budding Yeast)
TagsGSTExpressionBacterialMutationresidues 1-417 with deletion of 38-69PromoterT7Available SinceNov. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pITGFP89
Plasmid#83560PurposeUsed to evaluate the expression output of TEF1 promoter with yEGFP-CLN2PEST (destabilized GFP) used as the reporter gene.DepositorInsertTEF1 promoter -yEGFP-CLN2PEST
ExpressionYeastPromoterTEF1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
NAX_CAGGS_CAS9_m4_VPR_EYFP
Plasmid#167882PurposePiggyBac compatible plasmid expressing dCas9-VPRDepositorInsertdCas9-VPR
UseCRISPRMutationD10A, D839A, H840A, N863AAvailable SinceFeb. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
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IpO
Plasmid#48233PurposeBase vector for PCR-based DNA transplant in yeast. DNA segments to be transplanted into the yeast genome are cloned into the MCS.DepositorInsertsURA3 3' fragment
URA3 5' fragment
UseRoutine cloning vectorMutation-242 upstream to URA3 ORF +495 and URA3 ORF from …PromoterURA3 promoter and noneAvailable SinceFeb. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-Purg1-GFP
Plasmid#39353DepositorUseYeast genomic targetingTagsGFP and kanMX6 module - reverse orientationAvailable SinceNov. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pNB813
Plasmid#60161PurposeYeast codon-optimized yellow fluorescent protein (yEVenus) with PEST degron driven by SIC1 promoter.DepositorInsertyEVenus-PEST
TagsPEST degronExpressionYeastPromoterSIC1prAvailable SinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pXylA-RgTALsyn
Plasmid#100948PurposeExpression of Tyrosine Ammonia Lyase from constitutive XylA promoterDepositorInsertRgTALsyn
UseSynthetic BiologyExpressionBacterialMutationSilent mutation to remove internal NdeI site on T…PromoterXylA (Constitutive)Available SinceSept. 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGBDU-Atg17
Plasmid#121394PurposeFor Yeast-two-hybrid analysisDepositorInsertATG17
ExpressionYeastPromoterADH1 promoterAvailable SinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSK226
Plasmid#129268Purposedual yTRAP, RNQ1 fusion to aTF2 reporting on [RNQ+] state with mNeonGreen reporterDepositorInsertRnq1
ExpressionYeastAvailable SinceOct. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
POT10-RFP
Plasmid#65326Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT8-RFP
Plasmid#65324Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB2802
Plasmid#63655PurposepCfB2802 is a vector for multiple integrations at sites sharing homology with Ty1Cons1. The selective marker is Kl.LEU2. Additionally it contains a PTEF1-GFP as reporter.DepositorInsertGFP
UseCre/LoxExpressionYeastPromoterTEF1 promoterAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pEGFP_C1 I3
Plasmid#61397PurposeExpresses GFP-tagged Inhibitor-3 in mammalian cellsDepositorAvailable SinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
POT7-RFP
Plasmid#65323Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT6-RFP
Plasmid#65322Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBBK93 pHomL-TDH3p-Neon(gfp)-SSA1t-HomR (AmpR)
Plasmid#179077PurposeVector containing a yeast integration cassette for expressing TDH3p-Neon(gfp), an overexpressed (bright) marker for the cytosolDepositorInsertTDH3p-Neon(gfp)
ExpressionYeastAvailable SinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-kanMX6-3nmt1-mMaple3
Plasmid#105167PurposeYeast gene targetingDepositorInsertkanMX6-3nmt1-mMaple3
Tags3nmt1-mMaple3ExpressionBacterial and YeastPromoter3nmt1Available SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pCfB2990
Plasmid#63645PurposepCfB2990 is a vector for multiple integrations at sites sharing homology with Ty3Cons. The selective marker is Kl.LEU2. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Ec-6
Plasmid#163132PurposepLyGo cloning vector for periplasmic expression in E. coli of a sequence of interest (LPMO). Vector encoding PelB signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialPromoterT7Available SinceJuly 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTH353-SUP45wt
Plasmid#29371DepositorInsertSUP45 gene encoding translation release factor 1 from S. cerevisiae (SUP45 Budding Yeast)
ExpressionYeastMutationWild type gene including genomic sequence 1432 bp…Available SinceMay 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX4T2-Snf1-cat
Plasmid#52683Purposeexpression of the Snf1 kinase domain in bacteriaDepositorInsertSNF1 (SNF1 Budding Yeast)
TagsGSTExpressionBacterialMutationencodes kinase domain only, aa 41-315PromotertacAvailable SinceApril 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDUAL-Pnmt1-Ost4-GFP-MTn
Plasmid#154369PurposeYeast expression of Ost4DepositorInsertOst4 (ost4 Fission Yeast)
ExpressionYeastAvailable SinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
GST-TEVcs-ATG101
Plasmid#171414PurposeFor the purifcation of human ULK1 complexDepositorInsertATG101 (ATG101 Synthetic)
ExpressionMammalianAvailable SinceJuly 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406 ATG1-ATG17-3'UTR
Plasmid#166848PurposeExpresses Atg1 with ATG17 3'UTR in yeasts.DepositorAvailable SinceApril 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLyGo-Kp-2
Plasmid#163144PurposepLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-amylase signal peptide and the LyGo cassette (SapI-ccdB-SapI)DepositorTypeEmpty backboneUseSynthetic BiologyExpressionYeastPromoterAOX1Available SinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
POT11-RFP
Plasmid#65327Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS424-URR
Plasmid#65330Purposehigh copy number plasmid as receiving vector for exogenous pathwayDepositorInsertURR1-RFP-Leu2-URR2
UseSynthetic BiologyAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT5-RFP
Plasmid#65321Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pRS414-URR
Plasmid#65329PurposeCEN plasmid as receiving vector for exogenous pathwayDepositorInsertURR1-RFP-Leu2-URR2
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMV-URR2
Plasmid#65332Purposestandard plasmids for exogenus pathway assembly haboring recombination sequence 2 (Universal Recombination Region 2) which could be released and ligated to series of TUsDepositorInsertURR2
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT3-RFP
Plasmid#65319Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
POT9-RFP
Plasmid#65325Purposereceiving vector of standard transcription units(TUs) in YeastFab work, useful for further assemblyDepositorInsertRFP
UseSynthetic BiologyAvailable SinceFeb. 16, 2016AvailabilityAcademic Institutions and Nonprofits only