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pcDNA-Wnt1

Plasmid

#35905

Depositor

Marian Waterman

Publication

Najdi et al Differentiation. 2012 Sep;84(2):203-13.

Insert

Wnt1 (WNT1 Human)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available since

May 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pSH-EFIRES-P-AtAFB2

Plasmid

#129715

Purpose

AAVS1 safe harbor targeting vector expressing AtAFB2

Depositor

Elina Ikonen

Publication

Li et al Nat Methods. 2019 Aug 26. pii: 10.1038/s

Insert

AFB2 (AFB2 Mustard Weed)

Use

CRISPR and TALEN; Human safe harbor locus (a avs1…

Tags

no

Expression

Mammalian

Mutation

codon optimized

Promoter

EF1a

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pcDNA3/mStayGold(c4)=UtrCH

Plasmid

#212020

Purpose

For filamentous actin labeling. Alternatively, the UtrCH gene can be replaced with a target gene for N-terminal tagging with mStayGold via a c4 adaptor and a linker [(GGGGS)3] (denoted as ‘=’).

Depositor

Atsushi Miyawaki

Publication

Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

UtrCH

Tags

mStayGold(c4)

Expression

Mammalian

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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p4xmts-mScarlet3_N1

Plasmid

#189774

Purpose

Construct for labelling mitochondria in mammalian cells using mitochondrial targeted mScarlet3

Depositor

Dorus Gadella

Publication

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

4xmts-mScarlet3

Expression

Mammalian

Promoter

CMV

Available since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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AAV.CBA.eGFP.2A.wtTau

Plasmid

#140424

Purpose

AAV.CBA.eGFP.2A.wtTau propagation reporter WT-2N4R-hTau

Depositor

Bradley Hyman

Publication

Wegmann et al Sci Adv. 2019 Jun 26;5(6):eaaw6404. doi:

Insert

eGFP-2A-Tau (MAPT Human, Synthetic)

Use

AAV

Tags

eGFP

Promoter

CBA

Available since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUCmini-iCAP-PHP.V1

Plasmid

#127847

Purpose

non-standard AAV2 rep-AAV-PHP.V1 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

Depositor

Viviana Gradinaru

Publication

Ravindra Kumar et al Nat Methods. 2020 May;17(5):541-550. doi

Insert

Synthetic construct isolate AAV-PHP.V1 VP1 gene

Use

AAV

Expression

Mammalian

Promoter

p41

Available since

April 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pET_RTX-6xHis

Plasmid

#145029

Purpose

pET vector for IPTG-inducible, T7-based expression of RTX bearing a C-terminal 6xHis tag

Depositor

Andrew Ellington

Publication

Bhadra et al BioProtocol 2021

Insert

RTX

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLJC5-3XMyc-EGFP-PEX26

Plasmid

#139059

Purpose

Myc-PEROXO Lentiviral Construct. Expresses a Peroxisomal Tag.

Depositor

David Sabatini

Publication

Ray et al iScience. 2020 May 22;23(5):101109. doi:

Insert

3XMyc-EGFP-PEX26

Use

Lentiviral

Tags

Myc

Promoter

UBC

Available since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-ZNF598-TEV-3xFLAG

Plasmid

#105690

Purpose

Expresses C terminally 3xFLAG tagged ZNF598 protein

Depositor

Ramanujan Hegde

Publication

Juszkiewicz et al Mol Cell. 2017 Feb 16;65(4):743-750.e4.

Insert

ZNF598 (ZNF598 Human)

Tags

TEV-3xFLAG

Expression

Mammalian

Promoter

CMV (from vector)

Available since

Feb. 6, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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Lamp1-mTurquoise2

Plasmid

#98828

Purpose

In vivo visualization of lysosomes (can be used for colocalization studies)

Depositor

Dorus Gadella

Publication

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

Lamp1

Tags

mTurquoise2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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AAV-U6gRNA1-U6gRNA2-TnT-Cre

Plasmid

#87682

Purpose

AAV vector for U6 driven expression of two gRNAs, and cardiomyocyte specific expression of Cre recombinase.

Depositor

William Pu

Publication

Guo et al Circ Res. 2017 Mar 29. pii: CIRCRESAHA.1

Inserts

gRNA1
gRNA2
Cre

Use

AAV

Promoter

U6 and cTnT

Available since

March 30, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pGL4.0-TERT WT

Plasmid

#84924

Purpose

luciferase reporter for TERT promoter

Depositor

Joseph Costello

Publication

Bell et al Science. 2015 May 29;348(6238):1036-9. d

Insert

TERT promoter (TERT Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

TERT

Available since

March 8, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pGG-B1

Plasmid

#78504

Purpose

Trans plasmid for production of recombinant AAV-B1 vectors. Plasmid backbone may yield higher titers than pAR-B1 and encodes AAV2 Rep and AAV-B1 capsid

Depositor

Miguel Sena-Esteves

Publication

Choudhury et al Mol Ther. 2016 Apr 27. doi: 10.1038/mt.2

Inserts

AAV2 Rep
AAV-B1 capsid

Use

AAV

Expression

Mammalian

Available since

June 20, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Str-Ii_VSVG-SBP-mCherry

Plasmid

#65301

Purpose

synchronize trafficking of VSVG from the ER (RUSH system)

Depositor

Franck Perez

Publication

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

VSV-G

Tags

Streptavidin Binding Protein (SBP) and mCherry

Expression

Mammalian

Promoter

CMV

Available since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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p2attPC

Plasmid

#51547

Purpose

DICE donor plasmid without neural transcription factors.

Depositor

Michele Calos

Publication

Zhu et al Nucleic Acids Res. 2013 Dec 4.

Insert

mCherry

Expression

Mammalian

Available since

March 3, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pDZ415 (24MS2SL loxP-Kan-loxP)

Plasmid

#45162

Depositor

Robert Singer, Daniel Zenklusen

Publication

Hocine et al Nat Methods. 2013 Feb;10(2):119-21. doi:

Insert

24xMS2 Stem Loop Cassette

Available since

Aug. 8, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pMXs-IP-EGFP-hFIP200

Plasmid

#38192

Depositor

Noboru Mizushima

Publication

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

FIP200 (RB1CC1 Human)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available since

Nov. 27, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles
MSCV-N E4orf1

Plasmid

#38063

Depositor

Karl Munger

Publication

Rozenblatt-Rosen et al Nature. 2012 Jul 26;487(7408):491-5.

Insert

E4 orf1

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Promoter

PKG

Available since

Aug. 3, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCMX-Gal4-TEAD2

Plasmid

#33107

Depositor

Kunliang Guan

Publication

Zhao et al Genes Dev. 2008 Jul 15. 22(14):1962-71.

Insert

TEAD2 (TEAD2 Human)

Tags

Gal4

Expression

Mammalian

Available since

Feb. 10, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pLv-CMV-MyoD-ER(T)

Plasmid

#26809

Depositor

Jeffrey Chamberlain

Publication

Kimura et al Hum Mol Genet. 2008 Aug 15. 17(16):2507-

Insert

MyoD-ER(T) (Myod1 Mouse)

Use

Lentiviral

Available since

Sept. 12, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles