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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 10,599 results for: cat.1

Showing: 4881 - 4920 of 10599 results

  4. NLuc-His10

    Plasmid
    #234567
    Purpose
    Bacterially expressed NLuc for Ni-NTA purification
    Depositor
    James Inglese
    Article
    Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:
    Insert
    Nano luciferase
    Use
    Tags
    T7 gene 10 leader peptide and Thrombin site-10xHi…
    Expression
    Bacterial
    Mutation
    Codon optimized for bacterial expression
    Promoter
    T7
    Available since
    May 12, 2025
    Availability
    Academic Institutions and Nonprofits only
    234567-plasmid-map-sequence-id-466796

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  14. QUAS-0-T7-TetO-GFP_T7-LacO-TetR_ColE1

    Plasmid
    #171663
    Purpose
    QUAS directly upstream of a T7 promoter with a TetO operator. Expression of TetR in BL21(DE3) E. coli. GFP is expressed according to presence of aTc. Contains the ColE1 origin of replication.
    Depositor
    Tara Deans
    Article
    MacDonald et al Nat Commun. 2021 Jul 5;12(1):4109. doi:
    Insert
    QUAS-T7-TetO-GFP ssrA-T7 Stop- T7-LacO-TetR-T7 Stop
    Use
    Tags
    ssrA degradation tag (DAS+4)
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Oct. 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    171663-plasmid-map-sequence-id-339028

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  16. ATP1A1_G3_EGFP_Nick_Dual_sgRNA

    Plasmid
    #178095
    Purpose
    Control vector for coselection for PE3b in human cells. Tandem expression of ATP1A1 G3 and EGFP Nick sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + EGFP nick sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178095-plasmid-map-sequence-id-350125

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  17. pUAP12008

    Plasmid
    #177036
    Purpose
    Level 0 promoter and 5'UTR part for MoClo assembly, GGAG-CCAT
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    Cauliflower Mosaic Virus (CaMV) 35S promoter and Tobacco Mosaic Virus (TMV) omega 5' UTR
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    177036-plasmid-map-sequence-id-349003

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  18. pDEINO1

    Plasmid
    #179472
    Purpose
    Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation
    Depositor
    Bogumil Karas
    Article
    Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1
    Inserts
    ori
    cat
    Use
    Synthetic Biology; Bacterial and yeast cloning
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    179472-plasmid-map-sequence-id-375147

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  22. mRFP-mem

    Plasmid
    #55622
    Purpose
    This red fluorescent plasma membrane marker consists of the first twenty residues of neuromodulin fused to mRFP.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    mRFP-Mem
    Use
    Tags
    The N-terminal 20 residues of neuromodulin are fu…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55622-plasmid-map-sequence-id-86264

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  23. QUIN

    Plasmid
    #184267
    Purpose
    A binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.
    Depositor
    Feng Qu
    Article
    Zaulda et al Plant Methods. 2022 Oct 28;18(1):116. do
    Insert
    CPSMV RNA1 cDNA with 4 introns
    Use
    Tags
    Expression
    Plant
    Mutation
    S1098P, K1651R
    Promoter
    CaMV 35S with duplicated enhancer
    Available since
    June 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    184267-plasmid-map-sequence-id-363074

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  25. pM9-BetaGal-8C

    Plasmid
    #172489
    Purpose
    Plasmid encoding E. coli BetaGalactosidase with an N-terminal M9 nuclear localization sequence
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    M9-BetaGal-8C
    Use
    Tags
    6xHis and M9 nuclear localization sequence (from …
    Expression
    Bacterial
    Mutation
    BetaGal has two cysteine mutations (C77A, C1022S)…
    Promoter
    T7
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    172489-plasmid-map-sequence-id-356067

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  30. GFP-BICD2N

    Plasmid
    #64070
    Purpose
    N-terminal 400 amino acids of mouse BICD2 codon optimized for Sf9 expression with a N-terminal GFP tag and a TEV-cleavable His-ZZ tag for purification (GFP-BICD2N)
    Depositor
    Andrew Carter
    Article
    Schlager et al EMBO J. 2014 Sep 1;33(17):1855-68. doi:
    Insert
    BICD2N (1-400) (Bicd2 Mouse)
    Use
    Tags
    GFP and His-ZZ-TCS
    Expression
    Insect
    Mutation
    Codon optimized for Sf9 expression
    Promoter
    Available since
    Sept. 9, 2015
    Availability
    Academic Institutions and Nonprofits only
    64070-plasmid-map-sequence-id-123551

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  31. pEPQDKN0761

    Plasmid
    #185630
    Purpose
    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T which targets phytoene desaturase (PDS) of Nicotiana benthamiana, functionally equivalent to pEE393
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185630-plasmid-map-sequence-id-368586

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  32. ATP1A1_G3_RUNX1_Nick-38_Dual_sgRNA

    Plasmid
    #173214
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and RUNX1 nick-38 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 sgRNA + RUNX1 Nick-38 sgRNA
    Use
    CRISPR; Prime editing (pe3)
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Nov. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173214-plasmid-map-sequence-id-339507

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  33. ATP1A1_T804N_hPGK1_mScarlet-I-TOSI_Donor

    Plasmid
    #173208
    Purpose
    HDR donor plasmid to introduce the T804N mutation conferring cellular resistance to ouabain and the mScarlet-I mTOR signaling indicator (TOSI) cassette to ATP1A1 intron 17.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1-T804N mScarlet-I-TOSI donor
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    hPGK1
    Available since
    April 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    173208-plasmid-map-sequence-id-339517

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  38. ATP1A1_G3_MTOR_Nick_Intron-45_Dual_sgRNA

    Plasmid
    #178099
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G3 and MTOR Nick intron-45 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G3 + MTOR nick intron-45 sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178099-plasmid-map-sequence-id-350138

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  39. ATP1A1_G4_Q118R_MTOR_I2017T_Dual_pegRNA

    Plasmid
    #178103
    Purpose
    Control vector for coselection for prime editing in human cells. Tandem expression of ATP1A1 Q118R-G4 and MTOR-I2017T pegRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G4 Q118R pegRNA + MTOR-I2017T pegRNA
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178103-plasmid-map-sequence-id-350190

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  40. ATP1A1_G8_MTOR_Nick_Exon-44_Dual_sgRNA

    Plasmid
    #178105
    Purpose
    Control vector for coselection for PE3 in human cells. Tandem expression of ATP1A1 G8 and MTOR Nick exon-44 sgRNAs from two independent U6 promoters.
    Depositor
    Yannick Doyon
    Article
    Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:
    Insert
    ATP1A1 G8 + MTOR nick exon-44 sgRNAs
    Use
    CRISPR; Prime editing
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Tandem U6 promoters
    Available since
    Dec. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    178105-plasmid-map-sequence-id-350131

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Showing: 4881 - 4920 of 10599 results