We narrowed to 9,307 results for: control

1. FRB-GFP-Gab2(Y604F/Y634F)

   Plasmid

   #188659

   Purpose

   FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

   Depositor

   Andrei Karginov

   Article

   Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

   Insert

   GRB associated binding protein 2 (Gab2 Mouse)
   

   Tags

   FRB and GFP

   Expression

   Mammalian

   Mutation

   Y604F and Y634F

   Promoter

   CMV

   Available Since

   Aug. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. YFVdelCMENS1-Cre

   Plasmid

   #179954

   Purpose

   pCCI vector encoding YFV-17D lacking C, prM, E and NS1 proteins but with Cre

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   YFV-17D lacking C, M, E and NS1 proteins but with Cre
   

   Use

   Transneuronal tracing

   Promoter

   SP6

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ7751 pHR Ef1α: mCherry-P2A-AcrIIA4

   Plasmid

   #125148

   Purpose

   Lentiviral vector for constitutive expression of AcrIIA4 with mCherry fluorescent reporter.

   Depositor

   Stanley Qi

   Article

   Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

   Insert

   AcrIIA4-P2A-mCherry
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. (SUMO)5-(SIM)10

   Plasmid

   #126923

   Purpose

   Bacterial expression plasmid for multivalent scaffold of (SUMO)5-(SIM)10 biomolecular condensates to recruit SUMO-containing client proteins.

   Depositor

   Michael Rosen

   Article

   Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

   Insert

   5 repeats of human SUMO3 and 10 repeats of human SUMO interactive motif (SIM) from PIASx each separated by (GGS)4 (SUMO3 Human)
   

   Expression

   Bacterial

   Mutation

   internal BamHI site between (SUMO)5 and (SIM)10

   Promoter

   tac

   Available Since

   Sept. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPB-hCMV1-hKLF2-IV

   Plasmid

   #60433

   Purpose

   tet-inducible human KLF4

   Depositor

   Austin Smith

   Article

   Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

   Insert

   KLF2 (KLF2 Human)
   

   Expression

   Mammalian

   Available Since

   May 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEB1N-LZ-LOV2(fast)

   Plasmid

   #107690

   Purpose

   N-terminal half of π-EB1 with fast LOV2, no fluorescent tag

   Depositor

   Torsten Wittmann

   Article

   van Haren et al Nat Cell Biol. 2018 Jan 29. pii: 10.1038

   Insert

   MAPRE1 (MAPRE1 Human)
   

   Tags

   A. sativa phototropin 1 LOV2 domain (I427V, H519L…

   Expression

   Mammalian

   Mutation

   EB1 aa 1-185; resistant to EB1 shRNA#3 (Addgene #…

   Promoter

   CMV

   Available Since

   June 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. PB-empty-DSE-TetO-8kb

   Plasmid

   #213022

   Purpose

   piggyBac donor vector containing an 8-kb TetO operator array for random integration using PBase transposase

   Depositor

   Luca Giorgetti

   Article

   Mach et al Nat Genet. 2022 Dec;54(12):1907-1918. do

   Insert

   TetO
   

   Use

   Mouse Targeting; Piggybase integration (transposo…

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH732-2µ-RLuc/staCFLuc

   Plasmid

   #40607

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   The last three codons of the full-length Firefly …

   Promoter

   ADH1 and TDH3

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. J23100-mRFP1-331Bb

    Plasmid

    #78271

    Purpose

    mRFP1 behind constitutive promoter J23100 in pSEVA331Bb backbone

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    J23100-mRFP1
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLKO-puro FLAG wildtype, catalytic active lipin 1

    Plasmid

    #32010

    Depositor

    David Sabatini

    Article

    Peterson et al Cell. 2011 Aug 5;146(3):408-20.

    Insert

    lipin1 (Lpin1 Mouse)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pMRX-IP-SECFP-Galectin3

    Plasmid

    #64150

    Purpose

    Expression in mammalian cells (production of retrovirus vector)

    Depositor

    Tamotsu Yoshimori

    Article

    Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

    Insert

    Galectin3 (LGALS3 Human)
    

    Use

    Retroviral

    Tags

    SECFP

    Expression

    Mammalian

    Available Since

    April 17, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pRRLNeo-pEF1a-p53ashL344A-mCerulean

    Plasmid

    #69580

    Purpose

    Expresses p53-L344A mutant tagged with mCerulean

    Depositor

    Galit Lahav

    Article

    Gaglia et al Proc Natl Acad Sci U S A. 2013 Sep 17;11

    Insert

    p53-L344A (TP53 Human)
    

    Use

    Lentiviral

    Tags

    mCerulean

    Expression

    Mammalian

    Mutation

    p53 L344A mutation that prevents tetramerization …

    Promoter

    EF1alpha

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAY19 (OsTIR1(F74A) mAID-EGFP-NLS)

    Plasmid

    #140535

    Purpose

    OsTIR1(F74A)-P2A-mAID-EGFP-NLS TOL2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    mAID-EGFP-NLS TOL2
    

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Myc-Rab35 WT

    Plasmid

    #47433

    Depositor

    Peter McPherson

    Article

    Allaire et al J Cell Sci. 2013 Feb 1;126(Pt 3):722-31.

    Insert

    Rab35 (RAB35 Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3XFlag pCMV5-TOPO TAZ ∂CC

    Plasmid

    #24816

    Depositor

    Jeff Wrana

    Article

    Varelas et al Nat Cell Biol. 2008 Jul . 10(7):837-48.

    Insert

    TAZ (WWTR1 Human)
    

    Tags

    3XFlag

    Expression

    Mammalian

    Mutation

    Deletion of amino acids 222-265 (coiled coil doma…

    Available Since

    May 14, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

17. PEX-mCherry-CRY2

    Plasmid

    #102250

    Purpose

    Expresses fusion of CRY2PHR with mCherry and PEX3 (1-42)

    Depositor

    Bianxiao Cui

    Article

    Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

    Insert

    PEX-mCherry-CRY2 (PEX3, CRY2 Human, Mustard Weed)
    

    Tags

    6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pETM6-4F2-mCherry

    Plasmid

    #73421

    Purpose

    Reporter plasmid encoding mCherry, codon optimized for E. coli, transcriptionally driven by orthogonal T7-lac promoter variant 4F2.

    Depositor

    Mattheos Koffas

    Article

    Cress et al Nucleic Acids Res. 2016 Apr 13. pii: gkw

    Insert

    Promoter 4F2 (orthogonal T7-lac variant)
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    P4F2 (orthogonal T7-lac variant)

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTriEx-mVenus-PA-Rac1-T17N

    Plasmid

    #22017

    Purpose

    Expression of photoactivatable Rac1 (dominant-negative mutant)

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    PA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
    

    Tags

    6X His and mVenus

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Rac1 starts at I4 and contains mutation T17N.

    Promoter

    CMV, p10

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET28-MBP-TEV-AICD

    Plasmid

    #69927

    Purpose

    bacterial expression of MBP-TEV-AICD

    Depositor

    Zita Balklava, Thomas Wassmer

    Article

    Currinn et al Cell Mol Life Sci. 2015 Jul 28.

    Insert

    AICD (APP Human)
    

    Tags

    MBP-TEV

    Expression

    Bacterial

    Mutation

    47 aa long

    Promoter

    T7

    Available Since

    Oct. 29, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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21. His6tev CBP BCHZ (1079-1751)

    Plasmid

    #99341

    Purpose

    Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

    Depositor

    Peter Wright

    Article

    Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

    Insert

    mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
    

    Tags

    His6tev

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2+-Y276H PHYB-CAAX

    Plasmid

    #154911

    Purpose

    1-621 amino acids of Arabidopsis PHYB (as in construct Addgene ID 154910) with the CAAX membrane moiety tag. Tyrosine residue 276 of PHYB was mutated to Histidine.

    Depositor

    Jonathan Clarke, Clare Buckley

    Article

    Buckley et al Dev Cell. 2016 Jan 11;36(1):117-26. doi:

    Insert

    Phytochrome B (PHYB Mustard Weed)
    

    Use

    Xenopus/avian/zebrafish

    Tags

    CAAX membrane moiety and mCherry

    Expression

    Mammalian

    Mutation

    1-621 amino acids of Arabidopsis PHYB, Tyrosine r…

    Promoter

    CMV

    Available Since

    Aug. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-IV-GFPL10

    Plasmid

    #98704

    Purpose

    Cre-dependently express GFPL10 fusion using Introvert Switch

    Depositor

    Alexander Nectow, Jeffrey Friedman

    Article

    Nectow et al Cell. 2017 Jul 27;170(3):429-442.e11. do

    Insert

    GFPL10
    

    Use

    AAV and Cre/Lox

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    L10: 2609 C->T to remove EcoRI site

    Promoter

    EF1a

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. p3F-Strep-mMALT1

    Plasmid

    #33315

    Depositor

    Vanessa Redecke

    Article

    Stempin et al J Biol Chem. 2011 Oct 28;286(43):37147-5

    Insert

    Malt1 (Malt1 Mouse)
    

    Tags

    3xFLAG and StrepOne

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Dec. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

25. pTet01

    Plasmid

    #78283

    Purpose

    mRFP1 expressed behind an ATc-inducible construct

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    Tet inducible mRFP1
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. DBD (N208AH212R) Foxo1-WT pDNR-CMV

    Plasmid

    #17555

    Depositor

    Domenico Accili

    Article

    Kitamura et al J Clin Invest. 2007 Sep . 117(9):2477-85

    Insert

    Foxo1-WT-DBD (Foxo1 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    DNA binding-deficientChanged N208A H212R

    Promoter

    CMV

    Available Since

    Oct. 20, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pPB-PGK-hKLF4

    Plasmid

    #60435

    Purpose

    Over-expression of human KLF4

    Depositor

    Austin Smith

    Article

    Takashima et al Cell. 2014 Sep 11;158(6):1254-69. doi: 1

    Insert

    KLF4 (KLF4 Human)
    

    Expression

    Mammalian

    Available Since

    May 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLux02

    Plasmid

    #78282

    Purpose

    mRFP1 expressed behind an AHL-inducible construct

    Depositor

    Tom Ellis

    Article

    Florea et al Proc Natl Acad Sci U S A. 2016 Jun 14;11

    Insert

    AHL inducible mRFP1
    

    Available Since

    Nov. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCAG-mCherry-FKBP-GAP(ARHGAP29)

    Plasmid

    #222632

    Purpose

    Rho inactivation using rapamycin

    Depositor

    Arnold Hayer

    Article

    Marshall-Burghardt et al Sci Adv. 2024 Sep 6;10(36):eadn6858. doi

    Insert

    mCherry-FKBP-GAP(ARHGAP29) (ARHGAP29 Human)
    

    Tags

    mCherry-FKBP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pB80-KIF1A(1-365)-VVDfast-GFP-SSPB(micro)

    Plasmid

    #174631

    Purpose

    Opto-kinesin for optogenetic heterodimerization to iLID via SSPB(micro)

    Depositor

    Lukas Kapitein

    Article

    Nijenhuis et al J Cell Biol. 2020 Apr 6;219(4). pii: 133

    Insert

    KIF1A(1-365)-VVDfast-GFP-SSPB(micro) (Kif1a Synthetic, Mouse)
    

    Tags

    GFP-SSPB(micro)

    Expression

    Mammalian

    Mutation

    mmKIF1A(aa1-365): Pro202Ala; ncVVD(aa37-184): Ile…

    Promoter

    Chicken beta-actin

    Available Since

    Nov. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCAGGS-hPhyB621-mCherry-HRasCT

    Plasmid

    #100282

    Purpose

    Expresses Phytochrome B (1-621 aa) fused with mCherry and Hras C-terminus in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Insert

    Phytochrome B (1-621 aa) (PHYB Mustard Weed)
    

    Tags

    mCherry fused with HRas C-terminus for plasma mem…

    Expression

    Mammalian

    Mutation

    Human codon optimized PhyB (1-621 aa)

    Promoter

    CAG promoter

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pLVTHM-shRnd3

    Plasmid

    #86437

    Purpose

    2nd generation doxycycline-inducible lentiviral vector expressing Rnd3-targeting shRNA and GFP

    Depositor

    Violaine Moreau

    Article

    Grise et al Hepatology. 2012 Jun;55(6):1766-75. doi:

    Insert

    shRNA targeting RND3 (RND3 Human)
    

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    H1

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCRII-Topo Vglut2 in situ probe

    Plasmid

    #45639

    Depositor

    Jeffrey Macklis

    Article

    Azim et al Nat Neurosci. 2009 Oct;12(10):1238-47. d

    Insert

    Vglut2 in situ probe (Slc17a6 Mouse)
    

    Use

    In situ

    Mutation

    fragment contains nt 2477-2993 of slc17a6 (Vglut2…

    Available Since

    June 3, 2013

    Availability

    Academic Institutions and Nonprofits only

34. pGEM-HE-Glyco-Venus-bPAC(S27A)

    Plasmid

    #165490

    Purpose

    Xenopus oocyte expression of a photoactivatable adenylyl cyclase derived from bPAC using glycophorin for anchoring to the membrane with reduced dark activity

    Depositor

    Thomas Oertner

    Article

    Yang et al BMC Biol. 2021 Oct 18;19(1):227. doi: 10

    Insert

    Glyco-Venus-bPAC(S27A)-Myc
    

    Use

    Cdna expression, xenopus oocyte

    Tags

    Myc and Venus

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pX330-NL-DHFR-SpCas9

    Plasmid

    #124522

    Purpose

    Expresses SpCas9 fused to DHFR domains on both the N-terminus and an internal loop in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Manna et al Angew Chem Int Ed Engl. 2019 May 6;58(19

    Insert

    NL-DHFR-SpCas9
    

    Use

    AAV

    Tags

    Destabilized domain of E.coli dihydrofolate reduc…

    Expression

    Mammalian

    Promoter

    Cbh

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. KG#205

    Plasmid

    #110881

    Purpose

    Expresses the C. elegans pde-4 (isoform d) D448N cDNA pan-neuronally

    Depositor

    Kenneth Miller

    Article

    Charlie et al Genetics. 2006 May;173(1):111-30. doi: 1

    Inserts

    rab-3 promoter
    pde-4d (isoform d) D448N cDNA
    unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
    

    Expression

    Bacterial

    Mutation

    changed Asp 448 to Asn

    Available Since

    June 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCIBN-EYFP-hTRF2

    Plasmid

    #103804

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-EYFP-hTRF2 (CIB1, TERF2 Human, Mustard Weed)
    

    Expression

    Mammalian

    Mutation

    hTRF2: deletion of amino acids 1-42 and 476 compa…

    Promoter

    CMV

    Available Since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. KAT6B-Tol2

    Plasmid

    #113384

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of KAT6B gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    KAT6B-enhancer (KAT6B Human)
    

    Use

    Tol2 reporter

    Available Since

    Sept. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

39. LightR-iCre-miRFP670

    Plasmid

    #162158

    Purpose

    Expresses LightR-iCre-miRFP670 in mammalian cells

    Depositor

    Andrei Karginov

    Article

    Shaaya et al Elife. 2020 Sep 23;9. pii: 60647. doi: 1

    Insert

    LightR-iCre-miRFP670
    

    Tags

    NLS and miRFP670

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCDH-EFS-FlpO

    Plasmid

    #191509

    Purpose

    Lentiviral Flp Recombinase driven by the EFS promoter

    Depositor

    Eric Snyder

    Article

    Orstad et al Dev Cell. 2022 Aug 8;57(15):1866-1882.e1

    Insert

    flp recombinase (flp )
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a/EFS promoter inserted

    Available Since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge