We narrowed to 164,518 results for: DES
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Plasmid#79180PurposeA construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tagsDepositorInsertAdhesin Involved in Diffuse Adherence
TagsHis and MycExpressionBacterialPromoterlacUV5Available SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP VAMP2
Plasmid#42308DepositorInsertVAMP 2
TagsEGFPExpressionMammalianPromoterCMVAvailable SinceMarch 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
FUGW-PK-hLC3
Plasmid#61460PurposeExpress pHluorin-mKate2-hLC3 (PK-hLC3) in mammalian cells for monitoring autophagy, based on FUGW (3rd gen lentiviral plasmid)DepositorAvailable SinceFeb. 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-RB FL
Plasmid#16004DepositorAvailable SinceJune 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
N8his-GFPenhancer-GGGGS4-LaG16
Plasmid#140442PurposeWe designed a flexible linker to connect two types of anti GFP nanobodies and used this fusion nanobody to purify GFP tagged protein.DepositorInsertGFPenhancer-GGGGS4-LaG16
Tags8 histidine tagExpressionBacterialPromoterT7 promotorAvailable SinceMay 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLM-mCherry-Klf4
Plasmid#23243DepositorInsertmCherry_2A_KLF4 (KLF4 Human, synthetic fluorescent protein)
UseLentiviralAvailable SinceFeb. 5, 2010AvailabilityAcademic Institutions and Nonprofits only -
HA.PKD
Plasmid#10808DepositorAvailable SinceDec. 7, 2005AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM3D(Gq)-mCherry
Plasmid#50476PurposeGq-coupled hM3D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM3D(Gq)-mCherry (CHRM3 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-CIBN-CreC
Plasmid#26889PurposeExpresses C-terminal fragment of Cre fused to CIBN for blue light-induced activation of DNA recombination when used with CRY2-CreN. Also expresses mCherry.DepositorInsertCIBN-CreC
ExpressionMammalianMutationaa 1-170 of CIB1, aa 106-343 of CrePromoterCMVAvailable SinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
MSP1830
Plasmid#70708PurposeHuman expression plasmid for SaCas9 KKH variant: CAG-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAGDepositorInsertmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
UseCRISPRTagsNLS-3xFLAGExpressionMammalianMutationE782K, N968K and R1015H in SaCas9PromoterCAGAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-P2A-K0-P2A-RFP
Plasmid#105686PurposeDouble fluorescent stalling reporter K0DepositorInsertsP2A-3xFLAG-VHPbeta
K0
P2A-mCherry
ExpressionMammalianPromoterCMV (from vector)Available SinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf6 ActQ67L
Plasmid#10835DepositorInsertArf6 Q67L (ARF6 Human)
TagsHAExpressionMammalianMutationQ67L. Defective in GTP hydrolysis. Constitutive…Available SinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-SpRY-P2A-EGFP (RTW4830)
Plasmid#139989PurposeCMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFPDepositorInserthuman codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
UseCRISPR; In vitro transcription; t7 promoterTagsBPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationSpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…PromoterCMV and T7Available SinceMarch 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pRN227
Plasmid#127222PurposeBeYDV replicon with 35S::Cas9, WUS2 and AtSTM, sgRNA targeting PDSDepositorInsertCas9, WUS2, AtSTM, sgRNA
ExpressionPlantMutationcodon optimized CDSsAvailable SinceDec. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHelper_ShCAST_sgRNA
Plasmid#127921PurposeExpresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites).DepositorInsertShTnsB, ShTnsC, ShTniQ, ShCas12k, sgRNA scaffold for guide cloning (LguI sites)
Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH-spsgRNA2
Plasmid#64114PurposeVector for expression of Sp sgRNA2 in mammalian cellsDepositorInsertSp sgRNA2
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRsetB-his7-Perceval
Plasmid#20336DepositorInsertPerceval (fluorescent ATP:ADP sensor)
Tagshis(7)ExpressionBacterialAvailable SinceMarch 3, 2009AvailabilityAcademic Institutions and Nonprofits only -
10-4 cyclin E promoter
Plasmid#8458DepositorAvailable SinceJuly 28, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLX302_FOXA1-V5
Plasmid#70090PurposeExpression of human FOXA1 with C-terminal V5 tag, puromycin selection marker, 3rd generation lentiviral vectorDepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28c-F30-2xdBroccoli
Plasmid#66843PurposeExpresses two dimeric Broccoli aptamers in the F30 scaffold in bacterial cellsDepositorInsertTwo dimeric Broccoli aptamers in the F30 scaffold
ExpressionBacterialPromoterT7Available SinceJuly 13, 2015AvailabilityAcademic Institutions and Nonprofits only