Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

174,966 results

Showing: 2481 - 2500 of 174966 results

  2. Dendra2-Lifeact-7

    Plasmid
    #54694
    Purpose
    Localization: Actin, Excitation: 490 / 553, Emission: 507 / 573
    Depositor
    Michael Davidson
    Insert
    Lifeact-Dendra2
    Expression
    Mammalian
    Available Since
    July 25, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits
    54694-plasmid-map-sequence-id-138985

    Enlarge

  4. pAC94-pmax-dCas9VP160-2A-puro

    Plasmid
    #48226
    Purpose
    dCas9VP160-2A-puro (puro-selectable) on pmax expression vecor. Note: This is being tested.
    Depositor
    Rudolf Jaenisch
    Article
    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2
    Insert
    dCas9(D10A;H840A) fusion with VP160 activation domain followed by 2A-puro
    Use
    CRISPR
    Tags
    2A-puro, HA Tag, and VP160
    Expression
    Mammalian
    Mutation
    D10A;H840A (catalytically inactive)
    Promoter
    CAGGS
    Available Since
    Sept. 17, 2013
    Availability
    Academic Institutions and Nonprofits only
    48226-plasmid-map-sequence-id-69514

    Enlarge

  9. Cavb3

    Plasmid
    #26574
    Depositor
    Diane Lipscombe
    Insert
    calcium channel beta subunit-III (Cacnb3 Rat)
    Expression
    Mammalian
    Available Since
    Nov. 29, 2010
    Availability
    Academic Institutions and Nonprofits only
    26574-plasmid-map-sequence-id-148124

    Enlarge

  12. pCS2FLAG

    Plasmid
    #16331
    Depositor
    Peter Klein
    Insert
    Flag tag
    Use
    Vertebrate expression
    Tags
    Flag
    Mutation
    Flag tag inserted into pCS2+ polylinker
    Available Since
    Dec. 21, 2007
    Availability
    Academic Institutions and Nonprofits only
    16331-plasmid-map-sequence-id-316908

    Enlarge

  17. pCMV-T7-SpRY-P2A-EGFP (RTW4830)

    Plasmid
    #139989
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpRY(A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpRY with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N131…
    Promoter
    CMV and T7
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139989-plasmid-map-sequence-id-275392

    Enlarge

Showing: 2481 - 2500 of 174966 results