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pNatB (pACYCduet-naa20-naa25)

Plasmid

#53613

Purpose

Allows expression of the fission yeast NatB complex - chloramphenicol marker

Depositor

Dan Mulvihill

Publication

Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

Inserts

naa20 (naa20 Fission Yeast)
naa25 (naa25 Fission Yeast)

Expression

Bacterial

Promoter

T7

Available since

June 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

17 Articles

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pCDH-puro-myr-HA-Akt1

Plasmid

#46969

Purpose

lentiviral expression of myr-HA-Akt1

Depositor

Jialiang Wang

Publication

Cheng et al Clin Cancer Res. 2013 Apr 1;19(7):1748-5

Insert

AKT1 (AKT1 Human)

Use

Lentiviral

Tags

HA and myr

Expression

Mammalian

Promoter

CMV

Available since

Aug. 7, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

15 Articles

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LeGO-C2

Plasmid

#27339

Depositor

Boris Fehse

Publication

Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

Insert

SFFV promoter, mCherry

Use

Lentiviral

Expression

Mammalian

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

46 Articles

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IL6-GFP

Plasmid

#28088

Depositor

Jennifer Stow

Publication

Manderson et al J Cell Biol. 2007 Jul 2. 178(1):57-69.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

IL6 (Il6 Mouse)

Tags

EGFP

Expression

Mammalian

Available since

March 17, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pAAV-EF1a-FLEX-GTB

Plasmid

#26197

Depositor

Edward Callaway

Publication

Haubensak et al Nature. 2010 Nov 11. 468(7321):270-6.

Insert

Enhanced green fluorescent protein, TVA, rabies B19 glycoprotein

Use

AAV and Cre/Lox; Adeno-associated virus

Mutation

TVA, rabies glycoprotein are mammalian codon opti…

Available since

Nov. 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

14 Articles

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pDONR223-PDGFRB

Plasmid

#23893

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PDGFRB (PDGFRB Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pLM-mCerulean-cMyc

Plasmid

#23244

Depositor

Michel Sadelain

Publication

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCerulean-2A-MYC (MYC Human, synthetic fluorescent protein)

Use

Lentiviral

Available since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

7 Articles

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pEGFPN1-human Dynamin 1aa K44A

Plasmid

#22197

Depositor

Pietro De Camilli

Publication

Lee et al Proc Natl Acad Sci U S A. 2002 Jan 8. 99

Insert

human Dynamin 1aa K44A (DNM1 Human)

Tags

GFP

Expression

Bacterial and Mammalian

Mutation

K44A

Available since

Nov. 2, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

10 Articles

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pACAGW-ChR2-Venus-AAV

Plasmid

#20071

Purpose

AAV-mediated expression of ChR2-Venus for neuronal excitation

Depositor

Karel Svoboda

Publication

Leopoldo et al Nature. 2009 Jan 18.

Has service

AAV1 and AAV9

Insert

Channelrhodopsin-2 (CHLRE_02g085257v5 Chlamydomonas reinhardtii)

Use

AAV

Tags

Venus

Expression

Mammalian

Promoter

CAGG

Available since

Jan. 9, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

30 Articles

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pcDNA5/FRT/TO GFP

Plasmid

#19444

Depositor

Harm Kampinga

Publication

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GFP

Expression

Mammalian

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

8 Articles

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pCMVInt

Plasmid

#18935

Depositor

Michele Calos

Publication

Groth et al Proc Natl Acad Sci U S A. 2000 May 23. 9

Insert

phiC31 integrase (int Streptomyces phage phiC31)

Expression

Mammalian

Available since

Aug. 13, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pShuttle-Cre-HA

Plasmid

#16583

Depositor

Bert Vogelstein

Publication

Jallepalli et al Cell. 2001 May 18. 105(4):445-57.

Insert

cre recombinase (cre P1 bacteriophage)

Use

Adenoviral

Tags

HA

Expression

Mammalian

Available since

March 12, 2008

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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PGKneotpAlox2

Plasmid

#13444

Depositor

Philippe Soriano

Publication

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

PGKneotpAlox2

Use

Cre/Lox

Expression

Mammalian

Available since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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MDH1-PGK-GFP_2.0

Plasmid

#11375

Purpose

Retroviral construct for miRNA expression

Depositor

Chang-Zheng Chen

Publication

Chen et al Science. 2004 Jan 2. 303(5654):83-6.

Type

Empty backbone

Use

RNAi and Retroviral

Expression

Mammalian

Available since

Aug. 3, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

16 Articles

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1066 pBabe puroL PTEN

Plasmid

#10785

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Use

Retroviral

Expression

Mammalian

Available since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Plasmid

#179106

Purpose

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Depositor

Omar Abudayyeh, Jonathan Gootenberg

Publication

Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT

Use

CRISPR

Expression

Mammalian

Available since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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Dual-sgRNA_hU6-mU6

Plasmid

#154194

Purpose

Lentiviral expression plasmid for two sgRNAs from a hU6 and a mU6 promoter. The cloning site design allows a one-step cloning strategy of both sgRNAs. Expresses a Thy1.1 marker.

Depositor

Anna Obenauf

Publication

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pLVX-EF1alpha-SARS-CoV-2-orf3a-2xStrep-IRES-Puro

Plasmid

#141383

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Publication

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-orf3a (ORF3a SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available since

April 8, 2020

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

13 Articles

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pLenti-mCherry-Mango II x 24

Plasmid

#127587

Purpose

Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

Depositor

David Rueda

Publication

Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

Insert

mCherry-Mango II x24

Tags

mCherry

Expression

Mammalian

Promoter

pTRE Tet ON

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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Cbh_v5 AAV-ABE N-terminal

Plasmid

#137177

Purpose

AAV genome: expresses the N-terminal of v5 AAV-ABE from the Cbh promoter

Depositor

David Liu

Publication

Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do

Insert

v5 AAV-ABE N-terminal

Use

AAV

Mutation

Cas9 D10A

Promoter

Cbh

Available since

Jan. 30, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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