We narrowed to 13,298 results for: EGFP

1. HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1

   Plasmid

   #222992

   Purpose

   Mammalian expression of iNme2Cas9 construct with EGFP-targeting sgRNA

   Depositor

   Jennifer Doudna

   Article

   Eggers et al Cell. 2024 May 13:S0092-8674(24)00457-4.

   Insert

   HuEx-1xNLS-iNme2Cas9-npNLS_EGFP-T1
   

   Use

   CRISPR

   Tags

   Puro

   Expression

   Mammalian

   Mutation

   Promoter

   pCAG

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-C1-GFP-SF3B6-FLAG

   Plasmid

   #86870

   Purpose

   To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   SF3B6 (SF3B6 Human)
   

   Use

   Tags

   EGFP and FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET19b-EcMscL-TEV-mEGFP

   Plasmid

   #165097

   Purpose

   Expresses EcMscLGFP as a fusion protein in E. coli BL21or using in vitro expression.

   Depositor

   Neha Kamat

   Article

   Jacobs et al Proc Natl Acad Sci U S A. 2019 Feb 13. p

   Insert

   E. Coli Mechanosensitive Channel of Large Conductance (mscL E. coli)
   

   Use

   Synthetic Biology

   Tags

   monomeric Enhanced Green Fluorescent Protein

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-C1 beta-catenin Y654F

   Plasmid

   #16072

   Depositor

   Erin Schuman

   Article

   Murase et al Neuron. 2002 Jul 3. 35(1):91-105.

   Insert

   beta-catenin (CTNNB1 Chicken)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Y654F, *(see below)

   Promoter

   Available since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA5FRT-EF-Pdgfralpha-EGFPN D842V

   Plasmid

   #66789

   Purpose

   Expression of constitutively active murine PDGFRalpha D842V tagged with GFP under the control of EF1a promoter

   Depositor

   Lotte Pedersen

   Article

   Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

   Insert

   PDGFRalpha (Pdgfra Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   D842V (constitutively active)

   Promoter

   EF1a

   Available since

   July 14, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pCDNA3.0_nucLAMA-G97 IRES EGFP

   Plasmid

   #136620

   Purpose

   Plasmid encoding nuclear localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and EGFP

   Depositor

   Kai Johnsson

   Article

   Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

   Insert

   SNAP-Tag-LAMA-G97-NLS (IRES EGFP)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5-FRT/TO-eGFP-SHLD3

   Plasmid

   #114125

   Purpose

   Expresses N-terminally tagged eGFP-SHLD3 in mammalian cells

   Depositor

   Daniel Durocher

   Article

   Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

   Insert

   SHLD3 (SHLD3 Human)
   

   Use

   Genomic integration, tetracycline inducible

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV/TetO2

   Available since

   Aug. 16, 2018

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pEGFP-NMHC-IIA-S1916A

   Plasmid

   #101040

   Purpose

   Expresses GFP-MYH9 construct (human myosin IIA) carrying mutation of serine 1916 to alanine, driven by CMV promoter. Thus inhibits PKC phosphorylation of the tail. Also carries S1915A mutation.

   Depositor

   Tom Egelhoff

   Article

   Rai et al J Biol Chem. 2017 Feb 24;292(8):3099-311

   Insert

   Non-muscle myosin IIA heavy chain (MYH9 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   S1916A, S1917A

   Promoter

   CMV

   Available since

   Nov. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-eGFP-NLS-RNF168

   Plasmid

   #133977

   Purpose

   mammalian expression vector of eGFP tagged wild type RNF168

   Depositor

   Daniel Durocher

   Article

   Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

   Insert

   RNF168 (RNF168 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

   Promoter

   CMV

   Available since

   Nov. 4, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pRK5-mEGFP-PHOX2B-MUT

    Plasmid

    #194577

    Purpose

    Mammalian Expression of mEGFP-PHOX2B Mutant (S207Afs*102) Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

    Insert

    PHOX2B (PHOX2B Human)
    

    Use

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    S207Afs*102

    Promoter

    Available since

    Feb. 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. CVS-N2c-nl.EGFP-SypGFP

    Plasmid

    #172380

    Purpose

    Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

    Depositor

    Peter Jonas, Yoav Ben-Simon

    Article

    Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

    Insert

    nuclear-localized EGFP + SypEGFP
    

    Use

    Neurotropic virus

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMR506-EF1a-H2B-EGFP

    Plasmid

    #186627

    Purpose

    Expresses nuclear-localised EGFP under EF1a promoter. For insertion of genetic barcodes into 3'UTR of EGFP.

    Depositor

    Jonas Frisén

    Article

    Ratz et al Nat Neurosci. 2022 Mar;25(3):285-294. do

    Insert

    EF1a (EEF1A1 Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-PDGFRb-IRES2-EGFP

    Plasmid

    #204354

    Purpose

    Expresses wild-type PDGFRb gene.

    Depositor

    Hirofumi Nakatomi

    Article

    Shima et al Sci Transl Med. 2023 Jun 14;15(700):eabq

    Insert

    Platelet derived growth factor receptor beta (PDGFRB) (PDGFRB Human)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBS595 tet-hCMV-EGFPcre

    Plasmid

    #11957

    Depositor

    Brian Sauer

    Article

    Le et al Anal Biochem. 1999 Jun 1. 270(2):334-6.

    Insert

    EGFP-cre (cre bacteriophage P1)
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-eGFP-NMHC-IIAΔtailpiece

    Plasmid

    #35689

    Depositor

    Tom Egelhoff

    Article

    Breckenridge et al Mol Biol Cell. 2009 Jan;20(1):338-47. Ep

    Insert

    MHC IIA (MYH9 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Added stop codon after codon 1928, eliminating th…

    Promoter

    CMV

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

16. AAV-DIO-TC66T-2A-EGFP-2A-oG

    Plasmid

    #178429

    Purpose

    Expresses TVA(TC66T), EGFP, and optimized Rabies G (oG) protein in a FLEX cassette

    Depositor

    Edward Callaway

    Inserts

    TVA950 Glu66→Thr
    Optimized G
    EGFP
    

    Use

    AAV and Cre/Lox; Adeno-associated virus

    Tags

    Expression

    Mutation

    Promoter

    hSyn

    Available since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1(-)-EGFP-FP4-mito

    Plasmid

    #229692

    Purpose

    Transient expression of EGFP FP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Mitochondira FP4 motifs from Listeria ActA
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1(-)-EGFP-AP4-mito

    Plasmid

    #229693

    Purpose

    Transient expression of EGFP AP4-mito in mammalian cells

    Depositor

    Alpha Yap

    Article

    Scott et al Mol Biol Cell. 2006 Mar;17(3):1085-95. d

    Insert

    EGFP; Control construct with tandem AP4 motifs (mitochondria)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. 223 pCS EGFP DEST

    Plasmid

    #13071

    Depositor

    Nathan Lawson

    Article

    Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 18, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAVS1-PuroR Xlone eGFP

    Plasmid

    #179837

    Purpose

    Doxycycline-inducible expression of eGFP

    Depositor

    Xiaoping Bao

    Article

    Jung et al ACS Synth Biol. 2022 May 24. doi: 10.102

    Insert

    eGFP
    

    Use

    CRISPR, Synthetic Biology, and TALEN ; Donor plas…

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3GS

    Available since

    June 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNL4-3 envFS eGFP gag2xFS

    Plasmid

    #101341

    Purpose

    1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

    Depositor

    Jeremy Luban

    Article

    McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

    Insert

    NL4-3
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. eGFP Y93H TC editing reporter

    Plasmid

    #198882

    Purpose

    Express eGFP fluorescence after Y93H correction and mCherry as transfection control

    Depositor

    Reuben Harris

    Article

    Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

    Insert

    enhanced green fluorescent protein
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Y93H

    Promoter

    CMV

    Available since

    Nov. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pDNA5-FRT/TO-eGFP-TONSL

    Plasmid

    #221012

    Purpose

    mammalian expression vector of eGFP tagged TONSL siRNA resistant

    Depositor

    Daniel Durocher

    Article

    O'Donnell et al Mol Cell. 2010 Nov 24;40(4):619-31. doi:

    Insert

    TONSL (TONSL Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    silent mutations in TONSL: 5’-gaATtAgaTCtaTCGatga…

    Promoter

    CMV

    Available since

    June 24, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pCSIIbleo-iRFP670-P2A-EGFP

    Plasmid

    #198060

    Purpose

    The lentiviral vector for iRFP670 and EGFP

    Depositor

    Michiyuki Matsuda

    Insert

    iRFP670
    

    Use

    Lentiviral

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET22b-mEB1-eGFP-6xHis

    Plasmid

    #130981

    Purpose

    Bacterial expression of mouse EB1-GFP-6xHis for protein purification.

    Depositor

    Anne Straube

    Article

    Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

    Insert

    EB1-GFP-6xHis (Mapre1 Mouse)
    

    Use

    Tags

    eGFP-6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pSG25-pBAD-tetO-deGFPssrA

    Plasmid

    #102452

    Purpose

    Expresses deGFP fluorescent protein with ssrA degradation tag in E. coli. The expression is controlled by a pBAD-tetO combinatorial promoter.

    Depositor

    Richard Murray

    Article

    Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

    Insert

    deGFP
    

    Use

    Tags

    ssrA

    Expression

    Bacterial

    Mutation

    Promoter

    pBAD-tetO

    Available since

    Oct. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pHX_HDR_hPAX7_IRES-EGFP-PGK-Neo

    Plasmid

    #160458

    Purpose

    Homology-directed recombination donor vector for EGFP reporter knock-in to human PAX7 3' UTR

    Depositor

    April Pyle

    Article

    Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

    Inserts

    PAX7 Left/5' Homology Arm (PAX7 Human)
    PAX7 Right/3' Homology Arm (PAX7 Human)
    

    Use

    Insertion of a fluorescent reporter through homol…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pLenti-X1-Blast-rLAMP1-eGFP

    Plasmid

    #109028

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    rLAMP1
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCW-eGFP-FHA-SHLD2

    Plasmid

    #114121

    Purpose

    Lentiviral vector for inducible expression of N-terminally tagged eGFP-RNF8 FHA domain-SHLD2; 53BP1-independent recruitment to damaged chromatin

    Depositor

    Daniel Durocher

    Article

    Noordermeer et al Nature. 2018 Aug;560(7716):117-121. doi:

    Insert

    SHLD2 (SHLD2 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Tags

    RNF8 FHA domain (aa 2-160) and eGFP

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet ON

    Available since

    Aug. 28, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pcDNA-EGFP-ELYS-polyA

    Plasmid

    #59746

    Purpose

    Express EGFP-ELYS in mammalian cells (CMV promoter). Also suitable for in vitro transcription (T7 promoter) for injecting mRNA into oocytes.

    Depositor

    Yi Zhang

    Article

    Inoue et al Nat Struct Mol Biol. 2014 Jul;21(7):609-

    Insert

    EGFP-ELYS
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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