Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

161,398 results

Showing: 2501 - 2520 of 161398 results

  1. pCMV-T7-ABEmax-SpRY

    Plasmid
    #195279
    Purpose
    Allows for in vitro transcription of original pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP (RTW5025) without EGFP
    Depositor
    Carsten Lederer, Petros Patsali
    Insert
    human codon optimized ABEmax(7.10) SpCas9 variant named SpRY without EGFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    June 1, 2023
    Availability
    Academic Institutions and Nonprofits only
    195279-plasmid-map-sequence-id-394869

    Enlarge

  6. pSUC01

    Plasmid
    #177204
    Purpose
    chromogenic reporter for gene knockout in Streptomyces
    Depositor
    Wenjun Guan
    Article
    Dong et al J Microbiol Biotechnol. 2021 Dec 28;31(1
    Insert
    SaindC
    Use
    Synthetic Biology
    Tags
    NONE
    Expression
    Bacterial
    Mutation
    NONE
    Promoter
    TGTTCACATTCGAACGGTCTCTGCTTTGACAACATGCTGTGCGGTGTTG…
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    177204-plasmid-map-sequence-id-382362

    Enlarge

  7. lenti-sgRNA hygro

    Plasmid
    #104991
    Purpose
    This 3rd generation lentiviral plasmid expresses a S. pyogenes CRISPR chimeric RNA element with customizable sgRNA from a U6 promoter and hygromycin resistance from an EF-1a promoter.
    Depositor
    Brett Stringer
    Article
    Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    104991-plasmid-map-sequence-id-335144

    Enlarge

  10. pGEX6P1-N-FLAG

    Plasmid
    #119759
    Purpose
    Allows cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag, and a retained N-terminal FLAG tag.
    Depositor
    Andrew Jackson, Martin Reijns
    Type
    Empty backbone
    Use
    Tags
    FLAG and GST
    Expression
    Bacterial
    Mutation
    Promoter
    tac promoter
    Available since
    Jan. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    119759-plasmid-map-sequence-id-233663

    Enlarge

  11. pAS088

    Plasmid
    #211502
    Purpose
    AAV genome backbone for AAV-Perturb-seq with direct gRNA capture and nuclei sorting based on eGFP.
    Depositor
    Randall Platt
    Article
    Santinha et al Nature. 2023 Oct;622(7982):367-375. doi:
    Type
    Empty backbone
    Use
    AAV, CRISPR, Cre/Lox, and Mouse Targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    211502-plasmid-map-sequence-id-417793

    Enlarge

  15. pSG5 PPAR alpha

    Plasmid
    #22751
    Depositor
    Bruce Spiegelman
    Insert
    PPARalpha (Ppara Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 3, 2009
    Availability
    Academic Institutions and Nonprofits only
    22751-plasmid-map-sequence-id-160481

    Enlarge

  16. pCas9cur

    Plasmid
    #71538
    Purpose
    Constitutive expression of Cas9 gene and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    Plasmid curing system
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    Feb. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    71538-plasmid-map-sequence-id-129198

    Enlarge

  18. pSV40-mCherry-P2A-Hygro

    Plasmid
    #106478
    Purpose
    Expression of mCherry and Hygromycin resistance gene from SV40 promoter
    Depositor
    Alex Hewitt
    Inserts
    mCherry
    Hygromycin B resistance
    Use
    Tags
    Expression
    Mammalian and Plant
    Mutation
    Promoter
    SV40 promoter
    Available since
    April 9, 2018
    Availability
    Academic Institutions and Nonprofits only
    106478-plasmid-map-sequence-id-207986

    Enlarge

  20. LiOn-CAG∞RFP

    Plasmid
    #154016
    Purpose
    Vector based on the LiOn integration-coupled translational switch (Kumamoto et al bioRxiv 2019) expressing the fluorescent protein mRFP1 from a CAG promoter upon action of the piggyBac transposase
    Depositor
    Jean Livet
    Article
    Kumamoto et al Neuron. 2020 Aug 19;107(4):617-630.e6. d
    Insert
    mRFP1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    H210P
    Promoter
    CAG
    Available since
    Sept. 29, 2020
    Availability
    Academic Institutions and Nonprofits only
    154016-plasmid-map-sequence-id-308108

    Enlarge

Showing: 2501 - 2520 of 161398 results