165,307 results

1. pCAG-mTagBFP2

   Plasmid

   #122373

   Purpose

   Expresses mTagBFP2 in mammalian cells

   Depositor

   Hiroshi Ochiai

   Article

   Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

   Insert

   mTagBFP2
   

   Expression

   Mammalian

   Available Since

   June 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Ac5-STABLE2-neo

   Plasmid

   #32426

   Depositor

   Rosa Barrio, James Sutherland

   Article

   Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

   Insert

   Flag-mCherry-T2A-GFP-T2A-neo
   

   Expression

   Insect

   Promoter

   Actin5C

   Available Since

   Oct. 6, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. T7-VEE-mCherry

   Plasmid

   #193355

   Purpose

   Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

   Depositor

   Hans Schöler

   Article

   MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

   Insert

   mCherry
   

   Use

   Venezuelan equine encephalitis (vee) virus rna re…

   Expression

   Mammalian

   Promoter

   T7

   Available Since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-EF1a-iCreV

   Plasmid

   #140135

   Purpose

   Can be used to generate AAV virus that will express light-inducible site-specific iCreV recombinase

   Depositor

   Hongkui Zeng

   Article

   Yao et al Nat Methods. 2020 Mar 23. pii: 10.1038/s

   Has Service

   AAV PHP.eB and AAV1

   Insert

   iCreV
   

   Use

   AAV and Cre/Lox

   Expression

   Mammalian

   Promoter

   EF1alpha

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHAGE-BRAF-V600E

   Plasmid

   #116204

   Purpose

   Lentiviral expression of BRAF V600E

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   BRAF (BRAF Human)
   

   Use

   Lentiviral

   Mutation

   V600E

   Available Since

   Jan. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLX302

   Plasmid

   #25896

   Purpose

   3rd generation lentiviral Gateway destination vector. Puromycin selection, V5 tag.

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   V5

   Expression

   Mammalian

   Available Since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. pOpen-P45

   Plasmid

   #165570

   Purpose

   Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

   Depositor

   Drew Endy, FreeGenes Project, Jennifer Molloy

   Insert

   P45
   

   Use

   Synthetic Biology

   Available Since

   Aug. 23, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. sUPRa

   Plasmid

   #223242

   Purpose

   sUPRa is a dual promoter and two-color construct, which is designed to report the global unfolded protein response (UPR) and affords unbiased cell detection irrespective of UPR levels

   Depositor

   Colin Akerman

   Article

   Chakrabarty et al Sci Rep. 2024 Jul 1;14(1):14990. doi: 10

   Insert

   sUPRa
   

   Use

   AAV

   Promoter

   Custom

   Available Since

   Oct. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pKM-U6-reRNA-acceptor-2xBbsI

   Plasmid

   #205633

   Purpose

   Cloning and U6-drive expression of ISDra2 TnpB reRNAs

   Depositor

   Gerald Schwank

   Article

   Marquart et al Nat Methods. 2024 Sep 23. doi: 10.1038/s

   Insert

   ISDra2-TnpB reRNA with placeholder for guide cloning with BbsI
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Sept. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CD8-GFP-FKBP

    Plasmid

    #100751

    Purpose

    Expression of GFP-FKBP at the plasma membrane in mammalian cells. Plasma membrane targeting is via CD8 alpha chain.

    Depositor

    Stephen Royle

    Article

    Clarke et al Nat Commun. 2018 Jul 4;9(1):2604. doi: 1

    Insert

    CD8A (CD8A Human)
    

    Tags

    FKBP and GFP

    Expression

    Mammalian

    Mutation

    Truncated

    Promoter

    CMV

    Available Since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW-CCNE1-HA-puro

    Plasmid

    #212669

    Purpose

    Express tagged cyclin E1

    Depositor

    Tobias Meyer, Yumi Konagaya

    Article

    Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

    Insert

    CCNE1 (CCNE1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Promoter

    TRE

    Available Since

    Aug. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-PEmax-P2A-GFP

    Plasmid

    #180020

    Purpose

    Mammalian expression of SpCas9 PEmax prime editor with P2A-EGFP marker

    Depositor

    David Liu

    Article

    Chen et al Cell. 2021 Oct 28;184(22):5635-5652.e29.

    Insert

    PEmax-P2A-EGFP
    

    Tags

    P2A-EGFP, SV40 bpNLS, and c-Myc NLS

    Expression

    Mammalian

    Mutation

    Detailed in manuscript

    Promoter

    CMV

    Available Since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. MC4R

    Plasmid

    #233630

    Purpose

    encodes the human melanocortin 4 receptor, without any tags

    Depositor

    Christian Gruber

    Article

    Muratspahic et al ACS Pharmacol Transl Sci. 2023 Sep 26;6(

    Insert

    MC4R (MC4R Human)
    

    Expression

    Mammalian

    Promoter

    CMV/T7

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. MCP-NMS

    Plasmid

    #210705

    Purpose

    This plasmid express EF1a promoter driven MCP-NMS transactivation module

    Depositor

    Isaac Hilton

    Article

    Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

    Insert

    MCP fused to transactivation domain from Human transcription factor NRF2, MRTF-A and STAT1
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    N55K in MCP

    Promoter

    EF1a

    Available Since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-8xHis-Ub

    Plasmid

    #107392

    Purpose

    Mammalian expression 8xHis-Ub

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    8 tandem repeats of 6xHis-Ub (See comments below)
    

    Tags

    None

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. SIN40C.SFFV.Luciferase.IRES.GFP

    Plasmid

    #169308

    Purpose

    Constitutive overexpression of Luciferase cDNA with GFP as a reporter fluorescent protein

    Depositor

    Jan-Henning Klusmann

    Article

    Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

    Insert

    Luciferase
    

    Use

    Lentiviral

    Tags

    IRES-EGFP

    Promoter

    SFFV

    Available Since

    July 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. 8xNFAT-ZsG-hCD8

    Plasmid

    #153417

    Purpose

    NFAT-driven ZsGreen-1 reporter gene with human CD8ab gene

    Depositor

    Maki Nakayama

    Article

    Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

    Insert

    8x of NFAT binding motif follwed by ZsGreen-1 fluorescent protein gene (Cd8a )
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1 NPY pHLeon

    Plasmid

    #235176

    Purpose

    Plasmid expressing NPY tag with pH sensor, pHLeon (pH-Lemon with mNeonGreen), in mammalian cells

    Depositor

    Kate White

    Article

    Lin et al ACS Sens. 2024 Nov 22;9(11):6032-6039. d

    Insert

    NPY-pHLeon (NPY Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAV-U6+27-Tornado-F30-Pepper(TAR Variant-2)

    Plasmid

    #129405

    Purpose

    circular Pepper RNA

    Depositor

    Samie Jaffrey

    Article

    Wu et al Nat Methods. 2019 Sep;16(9):862-865. doi

    Insert

    circular F30-Pepper(TAR Variant-2)
    

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAc-sgRNA-Cas9

    Plasmid

    #49330

    Purpose

    Expresses sgRNA and Cas9-Puro in Drosophila S2 cells

    Depositor

    Ji-Long Liu

    Article

    Bassett et al Biol Open. 2013 Dec 10. pii: bio.2013712

    Inserts

    Cas9
    dU6-sgRNA
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Insect

    Mutation

    Human codon optimised

    Promoter

    Actin-5c and Drosophila U6

    Available Since

    Dec. 19, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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