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Plasmid#60364PurposePlasmid for expression of NLS-mCherry (control plasmid). Confers resistance to Puromycin. Expression is inducible in T-REx cells.DepositorInsertNLS-mCherry
UseTagsNLS from SV40 T antigen and mCherryExpressionMutationPromoterCMV-tetAvailable sinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
beta Synuclein-VC
Plasmid#89484Purposeexpresses human beta Synuclein fused to the C-terminal part of VenusDepositorInsertSNCB (SNCB Human)
UseTagsc-terminal part of VenusExpressionMammalianMutationPromoterCMVAvailable sinceMay 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCA12-pHR-SFFV-BFP-B23
Plasmid#199443PurposeExpression of BFP-B23 in mammalian cellsDepositorInsertB23 (NPM1 Human)
UseLentiviralTagsTagBFPExpressionMammalianMutationPromoterSFFVAvailable sinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pME-mTurquoise-P2A-iCre (JDW 920)
Plasmid#224518PurposeA Gateway compatible middle entry clone containing mTurquoise P2A iCre (codon optimized Cre)DepositorInsertmTurquoise-P2A-iCre
UseCre/LoxTagsExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6s
Plasmid#62812PurposepPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-NLS
Plasmid#118937PurposeUsed to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Localization Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/HA-FLAG RBP2 H483A
Plasmid#14801DepositorInsertRetinoblastoma Binding Protein 2 (KDM5A Human)
UseTagsFLAG and HAExpressionMammalianMutationChanged His 483 to Ala, R46WPromoterAvailable sinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-NES
Plasmid#118939PurposeUsed to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with Nuclear Exclusion Signal
UseTagsExpressionPlantMutationPromoterAvailable sinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRvTUB-mEGFP-NLS
Plasmid#205011PurposeThe vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJ609-dCas9-KR-puro
Plasmid#158478PurposeExpress nuclease dead Cas9 fused to KillerRed chromophore in mammalian cellsDepositorInsertdCas9-KR
UseCRISPRTagsKillerRedExpressionMammalianMutationCas9m4 (D10A+D839A+H840A+N863A)PromoterCMVAvailable sinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_eCBmut
Plasmid#164605PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCBmut in neuronsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseAAVTagsExpressionMutationS383T, F177APromoterhSynAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-SHMY-PAUF
Plasmid#182645PurposeMammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequenceDepositorInsertPancreatic adenocarcinoma upregulated factor (PAUF)
UseRetroviralTagsHis6-tag, N-terminal ER targeting sequence of hem…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-Flag-H2A K5-9R
Plasmid#63562PurposeExpression of human H2A with K5R and K9R tagged with FlagDepositorInsertH2A (H2AC20 Human)
UseTagsFlag (MDYKDDDDKVPRIQ)ExpressionMammalianMutationLysine 5 and Lysine 9 are now ArgininePromoterCMVAvailable sinceMarch 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Syn-VARNAM2-Kv2.1PR
Plasmid#195524PurposeRed fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationVARNAM SI linkerPromoterSynapsinAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-JEDI-1P-Kv-WPRE
Plasmid#202614PurposeSoma and AIS-localized genetically encoded voltage indicator (GEVI) JEDI-1P in AAV production vector for expression in excitatory glutamatergic neurons using the promoter CaMKIIDepositorInsertJEDI-1P-Kv
UseAAVTagsExpressionMammalianMutationPromoterCaMKIIaAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EF1a-DIO-tdTomato-WPRE
Plasmid#202610PurposeDouble floxed red fluorescent protein tdTomato in AAV production vector expressed under the mammalian promoter (EF1a)DepositorInserttdTomato
UseAAV and Cre/LoxTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNeptune2
Plasmid#51309PurposeExpresses far-red fluorescent protein mNeptune2 in mammalian cellsDepositorInsertmNeptune2
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 8, 2014AvailabilityAcademic Institutions and Nonprofits only -
CoxVIIIx2-OeNL(Ca2+)_18μ-pcDNA3
Plasmid#111924PurposeOrange color luminescent indicator for calcium signaling.Mitochondrial targeting signals of subunit VIII of human cytochrome c oxidase (CoxVIII) were fused at the N-terminus of OeNL(Ca2+)_18μDepositorInsertOeNL(Ca2+)_18μ
UseTagsCoxVIIIx2ExpressionMammalianMutationE67D, E104D, D133E at CaMPromoterCMVAvailable sinceJune 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-DIO-GRAB_eCB2.0mut
Plasmid#164607PurposeExpress the endocannabinoid non-responsive sensor GRAB_eCB2.0 in Cre positive neuronsDepositorInsertEndocannabinoid non-responsive sensor GRAB_eCBmut
UseAAVTagsExpressionMutationS383T, F177APromoterhSynAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-EGFP-anti-mouse-IgG(kappa)-nanobody
Plasmid#138132PurposeExpresses GST-tagged EGFP-anti-mouse-IgG(kappa)-nanobody in E. coli cells.DepositorInsertEGFP-anti-mouse-IgG(kappa)-nanobody
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJuly 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-fDIO-GRAB_eCB2.0
Plasmid#164610PurposeExpress the endocannabinoid sensor GRAB_eCB2.0 in FLP positive cellsDepositorInsertEndocannabinoid sensor GRAB_eCB2.0
UseAAVTagsExpressionMutationS383TPromoterEFSAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
PARP1-c009
Plasmid#216872PurposeBacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusionDepositorInsertPARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-VARNAM2-Kv2.1PR
Plasmid#195530PurposeRed fluorescent, negative response-polarity voltage indicator under the control of CaMKII promoter; soma-targetedDepositorInsertVARNAM2-Kv2.1 proximal restriction sequence
UseAAVTagsExpressionMammalianMutationVARNAM SI linkerPromoterCaMKIIAvailable sinceJan. 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6f
Plasmid#62811PurposepPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6f-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-HA-Flag-natT1R2
Plasmid#113945Purposemammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutininDepositorInsertT1R2 (TAS1R2 Human)
UseTagsFLAG and Signal/leader sequence from influenza he…ExpressionMammalianMutationresidues 22-839PromoterCMVAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only -
pT2-7xTcf-NLS-CNL
Plasmid#65712PurposeTol2-based Wnt signal reporter plasmid (expresses NLS-CNL)DepositorInsert7xTcf, minCMV, 3xNLS, CNL
UseLuciferaseTagsExpressionMutationPromoterAvailable sinceFeb. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Per2:iLuc
Plasmid#160293Purposetarget Per2 gene with a color-switching luciferase cassette in mouse genome and the transfection of the recombinant DNA will lead genomic recombination and make a knock-in allele in mouse ES cells.DepositorInsertPer2LUC
UseTagsExpressionMammalianMutationPromoterno promotorAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-p160MBP
Plasmid#41DepositorInsertp160 myb binding protein (Mybbp1a Mouse)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2005AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/TO-UBE2QL1-Strep/HA
Plasmid#124666PurposeExpresses UBE2QL1 with C-terminal Strep-HA tag in mammalian cellsDepositorInsertUBE2QL1 (UBE2QL1 Human)
UseTags2xStrep/HAExpressionMammalianMutationPromoterCMVAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only