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Showing: 361 - 390 of 22143 results

  3. pIF251

    Plasmid
    #133935
    Purpose
    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda. The selection marker will recombineer 21.3-22.8 kb away from the cosL DNA end of the phage genome.
    Depositor
    IIya Finkelstein
    Article
    Kim et al Sci Rep. 2017 May 18;7(1):2071. doi: 10.
    Insert
    Kanamycin selection marker flanked by ~200 bp of homology to bacteriophage lambda
    Use
    Λ-phage recombineering plasmid
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 3, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    133935-plasmid-map-sequence-id-269109

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  4. pSECRETS-B

    Plasmid
    #196987
    Purpose
    For SECRETS protocol to screen for gRNA activity and specificity: BsaI cassette for gRNA expression or for cloning (x-)gRNA librarys + off-target for SECRETS counter-selection.
    Depositor
    Eric Josephs
    Article
    Herring-Nicholas et al Commun Biol. 2024 Jan 12;7(1):86. doi: 1
    Insert
    Spy gRNA cassette
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pltetO1
    Available since
    Jan. 17, 2024
    Availability
    Academic Institutions and Nonprofits only
    196987-plasmid-map-sequence-id-389302

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  5. pAPV-FLAG-DsRed2-ER-5

    Plasmid
    #170620
    Purpose
    Expresses FLAG-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV tripeptide after SP cleavage.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-FLAG-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170620-plasmid-map-sequence-id-335780

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  7. pAPV-mEmerald-DsRed2-ER-5

    Plasmid
    #170681
    Purpose
    Expresses mEmerald-DsRed2 targeted to the ER lumen, but the encoded protein escapes from the ER into tubular ERGICs due to the N-terminal APV and poorly exposed KDEL.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-mEmerald-DsRed2-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170681-plasmid-map-sequence-id-335805

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  8. pAPV-DsRed2-mEmerald-ER-5

    Plasmid
    #170715
    Purpose
    Expresses DsRed2-mEmerald targeted to the ER lumen. The encoded protein is effectively retrieved back to the ER due to exposed KDEL despite the N-terminal APV.
    Depositor
    Ke Xu
    Article
    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.
    Insert
    APV-DsRed2-mEmerald-ER-5
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    170715-plasmid-map-sequence-id-335807

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Showing: 361 - 390 of 22143 results