We narrowed to 2,760 results for: FAS
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Plasmid#170312PurposePlasmid for generating Tetrahymena thermophila somatic knockout strains for Q22MS1 with Paromomycin selectionDepositorInsertQ22MS1 knockout
UseTetrahymena somatic gene knockout vectorAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22MS1-2xStrep
Plasmid#170314PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22MS1 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22MS1
TagsStrep-Tag IIExpressionInsectPromoterPolyhedrinAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-GFP (PX458)
Plasmid#48138PurposeCas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorHas ServiceCloning Grade DNAInserthSpCas9
UseCRISPRTags3XFLAG and GFPExpressionMammalianPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-GFP (PX461)
Plasmid#48140PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNADepositorInserthSpCas9n
UseCRISPRTags3XFLAG and GFPExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB) (PX460)
Plasmid#48873PurposeCas9n (D10A nickase mutant) from S. pyogenesDepositorHas ServiceCloning Grade DNAInserthSpCas9n
UseCRISPRTags3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462)
Plasmid#48141PurposeNOTE: A new version of this plasmid is now available. See Addgene plasmid 62987DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantPromoterCbhAvailable SinceOct. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
Plasmid#41863PurposeThis plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.DepositorArticleInsertU6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
UseCRISPR and LentiviralExpressionMammalianAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
GFP-Ub KO.G76V
Plasmid#11932DepositorInsertUbiquitin KO.G76V (RBX1 Human)
TagsGFPExpressionMammalianMutationConjugation deficient mutant. All 7 lysines of ub…Available SinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MNDU3-Luciferase-PGK-EGFP-WPRE
Plasmid#89608PurposeExpression of Luciferase in human cells. 3rd generation lentiviral vectorDepositorInsertLuciferase
UseLentiviralExpressionMammalianPromoterMNDU3Available SinceJuly 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7653_v2
Plasmid#126855Purpose7653 b scaffold from compatibility group 3, made by method II, containing 1 Zn-dependent self-cleaving DNAzyme cassette for linearisation into 7560 b scaffoldDepositorInsertII_CS3_7653_v2
UseSynthetic BiologyAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBluescript-IC3-FLAG-Tev-ZZ
Plasmid#170311PurposePlasmid for endogenous C-terminal tagging of the Tetrahymena thermophila Outer Dynein Arm intermediate chain 3 with FLAG and ZZ tags.DepositorInsertOuter Dynein Arm Intermediate Chain 3
TagsFLAG, ZZAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP
Plasmid#74171PurposeLentiviral vector for expression of heterologous genesDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMay 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSO221 Phis-72 BirA GFP
Plasmid#45100DepositorAvailable SinceJune 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
pALPS
Plasmid#90996PurposeLentiviral vector for expression of heterologous genesDepositorTypeEmpty backboneUseLentiviralExpressionMammalianPromoterSFFVAvailable SinceMay 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS3-Syn21-V5-mCherry-P10
Plasmid#104617PurposeExpresses V5 tagged mCherry upon binding of TALE3 to VAS3DepositorInsertSyn21-V5-mCherry-P10-MhcSA
TagsV5ExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
L1
Plasmid#21200DepositorUseRetroviralMutationMek2 K101A fused to ER*, catalytically inactiveAvailable SinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
pmEos3.1-N1 GBP
Plasmid#220492PurposeExpression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos3.1 to track GFP proteins of interest to perform Fluorescent intrabody Localization MicroscopyDepositorInsertGFP Binding Protein tagged with mEos3.1
TagsmEos3.1ExpressionMammalianAvailable SinceAug. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-Q22YU3-2xStrep
Plasmid#170315PurposePlasmid for insect cell expression of Tetrahymena thermophila Q22YU3 C-terminally tagged with 2x Strep-tag IIDepositorInsertQ22YU3 (TTHERM_00122270 Tetrahymena thermophila)
TagsStrep-Tag IIExpressionInsectPromoterPolyhedrinAvailable SinceJuly 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pre_II_CS3_7746_v3
Plasmid#126856Purpose7653 b scaffold from compatibility group 3, made by method II, containing 2 Zn-dependent self-cleaving DNAzyme cassette for lbisection into 2 3780 b scaffoldsDepositorInsertII_CS3_7746_v3
UseSynthetic BiologyAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS4-Syn21-FLAG-Cerulean-P10
Plasmid#104618PurposeExpresses FLAG tagged Cerulean upon binding of TALE4 to VAS4DepositorInserthsp70-Syn21-FLAG-mCerulean-P10
TagsFLAGExpressionInsectPromoterhsp70Available SinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
TagsHAExpressionInsectPromoterhsp70Available SinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Puro (PX459) V2.0
Plasmid#62988PurposeCas9 from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorHas ServiceCloning Grade DNAInserthSpCas9-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianPromoterCbhAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX330-U6-Chimeric_BB-CBh-hSpCas9
Plasmid#42230PurposeA human codon-optimized SpCas9 and chimeric guide RNA expression plasmid.DepositorArticleHas ServiceCloning Grade DNAInserthumanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianPromoterCBhAvailable SinceFeb. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9n(BB)-2A-Puro (PX462) V2.0
Plasmid#62987PurposeCas9n (D10A nickase mutant) from S. pyogenes with 2A-Puro, and cloning backbone for sgRNA (V2.0)DepositorInserthSpCas9n-2A-Puro
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationCas9 D10A nickase mutantAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^mCherry
Plasmid#118758PurposeVPS13A internally tagged with mCherry at residue 1372.DepositorAvailable SinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
VPS13A^Halo
Plasmid#118759PurposeVPS13A internally tagged with Halo at residue 1372.DepositorAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCR1316
Plasmid#111126PurposeExpresses FANCA.DepositorAvailable SinceOct. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-wt
Plasmid#12965DepositorAvailable SinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA
Plasmid#61592PurposeA human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).DepositorHas ServiceAAV6 and AAV8InserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianAvailable SinceFeb. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pX603-AAV-CMV::NLS-dSaCas9(D10A,N580A)-NLS-3xHA-bGHpA
Plasmid#61594PurposeA catalytically inactive SaCas9 (dSaCas9) with the D10A and N580A mutations.DepositorInserthSaCas9
UseAAV and CRISPRTags3xHA and NLSExpressionMammalianMutationD10A, N580AAvailable SinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1545_v1
Plasmid#126861Purpose4x 1545 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1545_v1
UseSynthetic BiologyAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS10_1033_v1
Plasmid#126860Purpose4x 1033 b long scaffolds divided by Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 10, made by method IV, yields linear 1033 b long scaffolds after Zn incubationDepositorInsertIV_CS10_1033_v1
UseSynthetic BiologyAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-Rac1-wt
Plasmid#12980DepositorAvailable SinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
QUIN
Plasmid#184267PurposeA binary plasmid derived from pCambia1300, with an insert that is a full-length cDNA of CPSMV RNA1 flanked by CaMV 35S promoter and terminator.DepositorInsertCPSMV RNA1 cDNA with 4 introns
ExpressionPlantMutationS1098P, K1651RPromoterCaMV 35S with duplicated enhancerAvailable SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFS16 Pmyo-3 NPP-9 mChBioFLAG
Plasmid#45099DepositorTagsBLRP, FLAG, and mCherryExpressionWormPromotermyo-3 and unc-119Available SinceJune 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pre_IV_CS8_7569_v1
Plasmid#126865Purpose7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubationDepositorInsertIV_CS8_7569_v1
UseSynthetic BiologyAvailable SinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDHblast-flx-sORF-Katushka
Plasmid#179457PurposeLentiviral vector encoding a CRE inducible Katushka geneDepositorInsertloxP-shuffledORF-loxP-Katushka
UseLentiviralPromoterCMVAvailable SinceMarch 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
Ub[9]-mRFP[1]
Plasmid#20249DepositorInsertubiquitin C (UBC Human)
TagsmRFPExpressionMammalianMutationchanged Stop codon 686 to GlycineAvailable SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
L1E-1
Plasmid#21193DepositorUseRetroviralMutationMek1 R4F fused to ER*, constitutive activeAvailable SinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pDDGFP-Leu2d-GgKDELR2
Plasmid#123618PurposeExpresses WT GgKDELR2 in S. cerevisiae cells using Galactose induction, -URA and -Leu selectionDepositorAvailable SinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
CC2
Plasmid#21207DepositorUseSubclone vectorMutationMek1 R4F fused to ER*, constitutive activeAvailable SinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
DN9
Plasmid#21209DepositorUseSubclone vectorMutationMek1 K97M fused to ER*, catalytically inactiveAvailable SinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
LDN
Plasmid#21195DepositorUseRetroviralMutationMek1 K97M fused to ER*, catalytically inactiveAvailable SinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLenti6.3/V5-DEST-PKCi-A120E-ΔPB1
Plasmid#56658Purposeexpresses constitutively active human PKCi with a deletion on the PB1 domainDepositorInsertHuman PKCi (PRKCI Human)
UseLentiviralTagsV5/HisMutationA120E and deletion of PB1 domain (aa 24–98)PromoterCMVAvailable SinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
M2E-1
Plasmid#21206DepositorUseSubclone vectorMutationdeletion of residues EQQKKRLE, S222D S226D in Mek…Available SinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
L2E-2
Plasmid#21194DepositorUseRetroviralMutationdeletion of residues EQQKKRLE, S222D S226D in Me…Available SinceJuly 24, 2009AvailabilityAcademic Institutions and Nonprofits only -
mycUb-25aa
Plasmid#20256DepositorInsertUbiquitin-25 amino acid extension (RBX1 Human)
TagsmycExpressionMammalianMutationUbiquitin fused to N-terminus of GFP. Glycine 76 …Available SinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1-nsp7
Plasmid#201026PurposeExpresses SARS-CoV-2 nsp7 in mammalian.DepositorInsertnon-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
ExpressionMammalianPromoterT7 promoterAvailable SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-nsp7
Plasmid#201025PurposeExpresses SARS-CoV-2 nsp7 under control of a tetracycline-inducible promoter in mammalian.DepositorInsertnon-structural protein 7 (ORF1ab Severe acute respiratory syndrome coronavirus 2, Synthetic)
ExpressionMammalianPromoterCMV-tet-ONAvailable SinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET PI-SceI C-His K301A
Plasmid#195611PurposeExpresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the K301A active site mutationDepositorInsertPI-SceI
ExpressionBacterialMutationA822G, A823C, A824TPromoterT7Available SinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only