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pY016 (pcDNA3.1-hLbCpf1)

Plasmid

#69988

Purpose

Expresses humanized LbCpf1

Depositor

Feng Zhang

Article

Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

Insert

hLbCpf1

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pLTR-RD114A

Plasmid

#17576

Depositor

Jakob Reiser

Article

Zhang et al J Virol. 2004 Feb . 78(3):1219-29.

Insert

RD114 envelope glycoprotein

Use

Lentiviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Industry, Academic Institutions, and Nonprofits

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Ai9

Plasmid

#22799

Purpose

Rosa26 targeting vector, Cre-dependent tdtomato expression. See article for more colors.

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed tdTomato

Use

Mouse Targeting

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pLenti CMV GFP Hygro (656-4)

Plasmid

#17446

Purpose

3rd gen lentiviral eGFP expression vector, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Has Service

Lentiviral Prep

Insert

GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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V5-miniTurbo-NES_pCDNA3

Plasmid

#107170

Purpose

expresses V5-tagged miniTurbo in the mammalian cytosol

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

miniTurbo (BirA mutant)

Expression

Mammalian

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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EGFP-alphasynuclein-WT

Plasmid

#40822

Depositor

David Rubinsztein

Article

Furlong et al Biochem J. 2000 Mar 15;346 Pt 3:577-81.

Insert

SNCA (SNCA Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

May 8, 2013

Availability

Academic Institutions and Nonprofits only

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hACE2

Plasmid

#1786

Purpose

Mammalian expression of human ACE2

Depositor

Hyeryun Choe

Article

Li et al Nature. 2003 Nov 27. 426(6965):450-4.

Has Service

Cloning Grade DNA

Insert

Angiotensin-converting enzyme 2 (ACE2 Human)

Tags

C9 (GTETSQVAPA)

Expression

Mammalian

Available Since

Feb. 14, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pLV-CMV-LoxP-DsRed-LoxP-eGFP

Plasmid

#65726

Purpose

Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

Depositor

Jacco van Rheenen

Article

Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

Insert

LoxP-DsRed-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti CMV TetR Blast (716-1)

Plasmid

#17492

Purpose

3rd gen lentiviral Tetracycline repressor expression vector, CMV promoter, Blast

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Tetracycline repressor

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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1036 pcDNA3 Myr HA Akt1

Plasmid

#9008

Purpose

Mammalian expression of human Akt1 with myr, HA tags

Depositor

William Sellers

Article

Ramaswamy et al Proc Natl Acad Sci U S A. 1999 Mar 2. 96

Insert

Akt1 (AKT1 Human)

Tags

HA and myr

Expression

Mammalian

Available Since

Nov. 2, 2005

Availability

Academic Institutions and Nonprofits only

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pBiFC-VN173

Plasmid

#22010

Purpose

Mammalian expression of a fluorescent protein fragment derived from Venus for BiFC and multicolor BiFC assays

Depositor

Chang-Deng Hu

Article

Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

Insert

VN173

Tags

FLAG

Expression

Mammalian

Mutation

Venus residus 1-172

Available Since

Sept. 28, 2009

Availability

Academic Institutions and Nonprofits only

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pmU6-gRNA

Plasmid

#53187

Purpose

Expresses the S. pyogenes sgRNA from the mouse U6 promoter

Depositor

Charles Gersbach

Article

Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

mouse U6

Available Since

June 25, 2014

Availability

Academic Institutions and Nonprofits only

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FHRE-Luc

Plasmid

#1789

Purpose

Reporter plasmid for FOXO3a. Forkhead responsive element with luciferase readout.

Depositor

Michael Greenberg

Article

Brunet et al Cell 1999 Mar 19;96(6):857-68.

Insert

FHRE (FOXO3 Human)

Use

Luciferase

Expression

Mammalian

Mutation

3 copies of FRE

Available Since

Jan. 5, 2006

Availability

Academic Institutions and Nonprofits only

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pCAGEN

Plasmid

#11160

Purpose

Mammalian expression vector for cloning and expressing your gene under the CAG promoter

Depositor

Connie Cepko

Article

Matsuda et al Proc Natl Acad Sci U S A. 2004 Jan 6. 10

Has Service

Cloning Grade DNA

Insert

modified multiple cloning sites

Expression

Mammalian

Available Since

April 13, 2006

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-GFP

Plasmid

#37825

Purpose

AAV-mediated expression of GFP under the CAG promoter

Depositor

Edward Boyden

Has Service

AAV CAP-B10, AAV CAP-B22, AAV MaCPNS1, AAV MaCPNS2, AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV6, AAV6 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, and AAV9-X1.1

Insert

GFP

Use

AAV; Adeno-associated virus

Expression

Mammalian

Mutation

N/A

Promoter

CAG

Available Since

Aug. 8, 2012

Availability

Academic Institutions and Nonprofits only

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GFP-Ub

Plasmid

#11928

Purpose

Used for in vivo monitoring of ubiquitin localization.

Depositor

Nico Dantuma

Article

Dantuma et al J Cell Biol. 2006 Apr 10. 173(1):19-26.

Insert

Ubiquitin (UBC Human)

Tags

GFP

Expression

Mammalian

Available Since

June 22, 2006

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DEST (w117-1)

Plasmid

#17454

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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FUW-OSKM

Plasmid

#20328

Purpose

Lentiviral plasmid expressing mouse Oct4, Sox2, Klf4 and cMyc for iPS cell generation

Depositor

Rudolf Jaenisch

Article

Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

Insert

Oct4-P2A-Sox2-T2A-Klf4-E2A-cMyc (Myc, Sox2, Pou5f1, Klf4 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 23, 2009

Availability

Academic Institutions and Nonprofits only

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1436 pcDNA3 Flag HA

Plasmid

#10792

Purpose

pcDNA3 vector with added Flag, HA tags

Depositor

William Sellers

Has Service

Cloning Grade DNA

Type

Empty backbone

Tags

FLAG and HA

Expression

Mammalian

Available Since

Dec. 7, 2005

Availability

Academic Institutions and Nonprofits only

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pRK5-EGFP-Tau

Plasmid

#46904

Purpose

expresses EGFP tagged Tau in mammalian cells

Depositor

Karen Ashe

Article

Hoover et al Neuron. 2010 Dec 22;68(6):1067-81. doi:

Insert

MAPT (MAPT Human)

Tags

EGFP

Expression

Mammalian

Mutation

This WT htau construct contains human four-repeat…

Promoter

CMV

Available Since

July 24, 2013

Availability

Academic Institutions and Nonprofits only

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