19,200 results

1. pLNCX chick src Y527F

   Plasmid

   #13660

   Depositor

   Joan Brugge

   Insert

   src Y527F (SRC Chicken)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Y527F. Constitutive active mutant.

   Promoter

   Available since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDY0181 Cargo EGFP with AttP Bxb1 site (Parent minicircle)

   Plasmid

   #179115

   Purpose

   Cargo EGFP with AttP Bxb1 site (Parent minicircle)

   Depositor

   Omar Abudayyeh, Jonathan Gootenberg

   Article

   Yarnall et al Nat Biotechnol. 2022 Nov 24. doi: 10.103

   Insert

   Cargo EGFP with AttP Bxb1 site
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET LIC cloning vector (2A-T)

   Plasmid

   #29665

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC019 - LwCas13a from Leptotrichia wadei

   Plasmid

   #91909

   Purpose

   Expresses LwCas13a for bacterial expression and contains backbone for spacer cloning

   Depositor

   Feng Zhang

   Article

   Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

   Insert

   LwCas13a and guide expression cassette
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pcDNA3_NSlmb-vhhGFP4

   Plasmid

   #35579

   Depositor

   Markus Affolter

   Article

   Caussinus et al Nat Struct Mol Biol. 2011 Dec 11;19(1):1

   Insert

   NSlmb-vhhGFP4 (slmb Fly)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. p3xFLAG-CMV10-hFIP200

   Plasmid

   #24300

   Depositor

   Noboru Mizushima

   Article

   Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

   Insert

   FIP200 (RB1CC1 Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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7. hCMV-IE1:Renilla

   Plasmid

   #118066

   Purpose

   Transcriptional unit for constitutive expression of Renilla in mammalian cells

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Insert

   CMV:GreenRenilla:bGHF
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXG237

   Plasmid

   #141281

   Purpose

   ATF4 translational reporter

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   uORF1/2 of ATF4 followed by mApple
   

   Use

   Lentiviral

   Tags

   mApple

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. moxGFP

   Plasmid

   #68070

   Purpose

   C-terminal fusion to moxGFP

   Depositor

   Erik Snapp

   Article

   Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

   Type

   Empty backbone

   Use

   Tags

   moxGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 12, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSL0527 (pDonor)

    Plasmid

    #130634

    Purpose

    Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_mCherry_P2A_Hygro

    Plasmid

    #135003

    Purpose

    Lentiviral vector to express mCherry or to clone and express gene of interest, under control of EF1a promoter. Barcodes compatible with detection in single cell RNA-seq may be inserted at HpaI site.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. mTagBFP2-Lysosomes-20

    Plasmid

    #55308

    Purpose

    Localization: Lysosome Membrane, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    LAMP1
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

13. pET21a-SerRS-His

    Plasmid

    #124118

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Seryl-tRNA ligase (serS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSF3-Flag-FRB-Cbir_Myc-NBir-FKBP

    Plasmid

    #90008

    Purpose

    Split-BioID plasmid: Expresses C-terminally tagged FRB-CBir[257-321] and N-terminally tagged NBir[2-256]-FKBP

    Depositor

    Julien Béthune

    Article

    Schopp et al Nat Commun. 2017 Jun 6;8:15690. doi: 10.

    Inserts

    NBirA*-linker-FKBP
    FRB-linker-CBirA*
    

    Use

    Retroviral; Flp/frt

    Tags

    FLAG and Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNFkB:eGFP

    Plasmid

    #44922

    Depositor

    John Rawls

    Article

    Kanther et al Gastroenterology. 2011 Jul;141(1):197-20

    Insert

    EGFP
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Tet-pLKO-puro-SOX2

    Plasmid

    #47540

    Purpose

    Tet inducible knockdown of SOX2.

    Depositor

    Charles Rudin

    Article

    Rudin et al Nat Genet. 2012 Oct;44(10):1111-6. doi:

    Insert

    SOX2 shRNA
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    April 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. spMSP1D1

    Plasmid

    #173482

    Purpose

    Expresses spMSP1D1 in Bl21

    Depositor

    Huan Bao

    Article

    Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

    Insert

    spMSP1D1
    

    Use

    Tags

    Histag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. THBS1-bio-His

    Plasmid

    #53417

    Purpose

    Expresses full-length Thrombospondin-1 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    THBS1 (THBS1 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTECH-chAcK3RS(IPYE)

    Plasmid

    #104069

    Purpose

    Expression plasmid encoding chAcK3RS(IPYE) and pylT

    Depositor

    David Liu

    Article

    Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

    Insert

    Plpp chAcK3RS(IPYE); PproK pyl
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Contains activating mutations V31I, T56P, H62Y, A…

    Promoter

    Plpp and PproK

    Available since

    Dec. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCS2_IRES2_EGFP_KCNQ2 var4

    Plasmid

    #173154

    Purpose

    For mammalian expression of KCNQ2 and creating variants

    Depositor

    Al George

    Article

    Simkin et al Elife. 2021 Feb 5;10. pii: 64434. doi: 1

    Insert

    KCNQ2 (KCNQ2 Human)
    

    Use

    Tags

    IRES2-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Simian CMV IE94

    Available since

    Sept. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pUC18T-mini-Tn7T-Gm-eyfp

    Plasmid

    #65031

    Purpose

    GmR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Insert

    eyfp
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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22. Ai139 (TIT2L-GFP-ICL-TPT) targeting vector

    Plasmid

    #114426

    Purpose

    Target a Cre-dependent GFP and a tdTomato-P2A-tTA2 expression cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    GFP, tdTomato, tTA2,
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TRE2, CAG

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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23. Halo-TNFa-RUSH

    Plasmid

    #166901

    Purpose

    RUSH cargo: Str-KDEL_IRES_Tumor Necrosis Factor alpha (TNFa)-SBP-Halo

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Weigel et al Cell. 2021 Apr 29;184(9):2412-2429.e16.

    Insert

    HaloTag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-PARP1-3xFlag-WT

    Plasmid

    #111575

    Purpose

    Expresses full length PARP1 in mammalian cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pSL0283 (pTnsABC)

    Plasmid

    #130633

    Purpose

    Expresses V. cholerae CAST TnsA, TnsB, and TnsC from a T7 promoter.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchTnsA, VchTnsB, VchTnsC
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pQE30-His-AlaRS

    Plasmid

    #124103

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Alanyl-tRNA ligase (alaS Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pNatB (pACYCduet-naa20-naa25)

    Plasmid

    #53613

    Purpose

    Allows expression of the fission yeast NatB complex - chloramphenicol marker

    Depositor

    Dan Mulvihill

    Article

    Johnson et al PLoS One. 2010 Dec 23;5(12):e15801. doi:

    Inserts

    naa20 (naa20 Fission Yeast)
    naa25 (naa25 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pcDNA3.1 Flag-HIF1B (#930)

    Plasmid

    #99916

    Purpose

    mammalian expression

    Depositor

    James Brugarolas

    Article

    Chen et al Nature. 2016 Nov 3;539(7627):112-117. do

    Insert

    HIF1B (ARNT Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLIB

    Plasmid

    #80610

    Purpose

    pLIB - Library vector (biGBac system for expression of protein complexes in insect cells)

    Depositor

    Jan-Michael Peters

    Article

    Weissmann et al Proc Natl Acad Sci U S A. 2016 May 10;11

    Type

    Empty backbone

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    polyhedrin

    Available since

    Oct. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGS DRR mCherry Donor EF1a BFP

    Plasmid

    #98896

    Purpose

    Homologous recombination donor for pDRR. Expresses mCherry after repait by HR.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    Neomycin-T2A-mCherry-GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge