We narrowed to 24,482 results for: Spr

1. PX458_SAP130_2

   Plasmid

   #86311

   Purpose

   Encodes gRNA for 3' target of human SAP130

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA against SAP130 (SAP130 Human)
   

   Use

   CRISPR

   Available Since

   Jan. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A_FokI-1x5

   Plasmid

   #63605

   Purpose

   Expresses FokI-dCas9 and gRNA

   Depositor

   Takashi Yamamoto

   Article

   Nakagawa et al BMC Biotechnol. 2015 May 22;15(1):33.

   Insert

   FokI-dCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   June 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAN-PBAD-sgRNA-VR

   Plasmid

   #62286

   Purpose

   expression of VR sgRNA from the arabinose-inducible promoter

   Depositor

   Christopher Voigt

   Article

   Nielsen et al Mol Syst Biol. 2014 Nov 24;10:763. doi:

   Insert

   VR sgRNA
   

   Use

   CRISPR

   Expression

   Bacterial

   Promoter

   pBAD

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pT7-gRNA:Tyr (albino)

   Plasmid

   #60966

   Purpose

   Expression vector of rat Tyr (albino) guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   Tyrosinase gRNA
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pT7-gRNA:Tyr (wild)

   Plasmid

   #60967

   Purpose

   Expression vector of rat Tyr (wild) guide RNA

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2014 Jun 26;5:4240. doi: 10.

   Insert

   Tyrosinase gRNA
   

   Use

   CRISPR

   Available Since

   Jan. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

6. MsMUT_sgRNA1

   Plasmid

   #111296

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA1 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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7. MsMUT_sgRNA3

   Plasmid

   #111297

   Purpose

   CRISPR KO murine MUT

   Depositor

   Vamsi Mootha

   Article

   Shen et al Cell. 2017 Nov 2;171(4):771-782.e11. doi

   Insert

   sgRNA3 against murine MUT
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK384.4

   Plasmid

   #155369

   Purpose

   dCas9 + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   

   Use

   CRISPR

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Availability

   Academic Institutions and Nonprofits only

   

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9. pXPR_011

   Plasmid

   #59702

   Purpose

   This lentiviral vector can be used to assay Cas9 activity.

   Depositor

   John Doench, David Root

   Article

   Doench et al Nat Biotechnol. 2014 Sep 3. doi: 10.1038

   Insert

   EGFP sgRNA
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Sept. 3, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pYPQ152

    Plasmid

    #69303

    Purpose

    Gateway entry vector with pco-dCas9-VP64

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-VP64 (Plant codon-optimized)
    

    Use

    CRISPR

    Mutation

    VP64 activator fusion; NLS fusion

    Available Since

    Sept. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCW-Cas9-2A-EGFP

    Plasmid

    #167928

    Purpose

    Doxycycline-inducible Cas9 expression tracked by EGFP fluorescence marker.

    Depositor

    Ronald Germain

    Article

    Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

    Insert

    T2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    tTRE, hPGK

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Cas9 sgRNA vector

    Plasmid

    #68463

    Purpose

    Cas9 sgRNA vector for cloning

    Depositor

    Su-Chun Zhang

    Article

    Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pYPQ153

    Plasmid

    #69304

    Purpose

    Gateway entry vector with pco-dCas9-3X(SRDX)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    pco-dCas9-3X(SRDX) (Plant codon-optimized)
    

    Use

    CRISPR

    Tags

    2XFLAG

    Mutation

    SRDX (X3) repressor fusion; NLS fusion

    Available Since

    Oct. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLX_311-KRAB-dCas9

    Plasmid

    #96918

    Purpose

    for CRISPRi, lentiviral expression of KRAB-dCas9 and BlastR. Also called pXPR_121

    Depositor

    William Hahn, John Doench, David Root

    Article

    Rosenbluh et al Nat Commun. 2017 May 23;8:15403. doi: 10

    Insert

    dCas9
    

    Use

    Lentiviral

    Tags

    KRAB

    Expression

    Mammalian

    Mutation

    dCas9

    Promoter

    EF1a

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pXR003: CasRx gRNA cloning backbone

    Plasmid

    #109053

    Purpose

    hU6-driven expression of guide RNAs compatible with CasRx. 5' processed DR followed by BbsI sites for guide cloning.

    Depositor

    Patrick Hsu

    Article

    Konermann et al Cell. 2018 Mar 8. pii: S0092-8674(18)302

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFYF1320 EGFP Site#1

    Plasmid

    #47511

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 1
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXPR_502

    Plasmid

    #96923

    Purpose

    for CRISPRa, lentiviral expression of gRNA scaffold using activator P65 HSF

    Depositor

    John Doench, David Root

    Article

    Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    June 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PiggyBac dCas9-VPR

    Plasmid

    #191269

    Purpose

    piggyBac integration and dox-inducible expression of VPR-dCas9-HA-tagBFP

    Depositor

    Joanna Wysocka

    Article

    Fuentes et al Elife. 2018 Aug 2;7. pii: 35989. doi: 10

    Inserts

    VPR-dCas9-HA-tagBFP
    rtTA3-IRES-PURO
    

    Use

    CRISPR

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Available Since

    Nov. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJYS1Ptac

    Plasmid

    #85545

    Purpose

    Constitutive trancription of FnCpf1 and recT in C.glutamitum, tac promoter

    Depositor

    Sheng Yang

    Article

    Jiang et al Nat Commun. 2017 May 4;8:15179. doi: 10.

    Inserts

    Cpf1
    recT
    

    Use

    CRISPR; Shuttle vector corynebacterium glutamicum…

    Expression

    Bacterial

    Promoter

    tac and unknown

    Available Since

    May 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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