We narrowed to 29,679 results for: REP

1. pRSFDuet-1_VNp-LZ-mNeongreen

   Plasmid

   #182392

   Purpose

   Bacterial expression of Vesicle Nucleating peptide-Leucine Zipper-mNeongreen fusion

   Depositor

   Dan Mulvihill

   Article

   Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

   Insert

   VNp-LZ-mNeongreen
   

   Use

   Tags

   Vesicle Nucleating peptide (VNp)

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPBbsr2-5102HRasCT(Picchu)

   Plasmid

   #209917

   Purpose

   A FRET biosensor for tyrosine kinase activity

   Depositor

   Michiyuki Matsuda

   Article

   Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

   Insert

   5102HRasCT
   

   Use

   Tags

   YPet and nTurquoise-GL

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_FLAG-G1 PylRS

   Plasmid

   #154763

   Purpose

   plasmid with G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available Since

   Sept. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCF822-recipient_U6-sgRNA-EFS-Cas9-P2A-Puro

   Plasmid

   #211687

   Purpose

   U6-sgRNA-EFS-Cas9-P2A-Puro

   Depositor

   Christof Fellmann

   Article

   Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

   Insert

   SpyCas9 and guide RNA recipient vector
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-KS-attB2-SA(1)-T2A-Gal4

   Plasmid

   #62900

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 1

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-CAGGS-Flex/5'USS-GFP

   Plasmid

   #197884

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBS-KS-attB2-SA(2)-T2A-Gal4

   Plasmid

   #62901

   Purpose

   Creating Gal4 drivers from MiMIC lines with inserts into coding introns in Phase 2

   Depositor

   Benjamin White

   Article

   Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

   Insert

   T2A-Gal4
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pscAAV-Tyr

   Plasmid

   #135616

   Purpose

   Donor vector for genomic targeting of an EcoRI restriction site to the mouse Tyrosinase locus

   Depositor

   Lin He

   Article

   Chen et al Cell Rep. 2019 Jun 25;27(13):3780-3789.e

   Insert

   EcoRI restriction site flanked by Tyr homology arms (Tyr Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLX301-KANK1

   Plasmid

   #121985

   Purpose

   overexpress of KANK1

   Depositor

   GuangJun Zhang

   Article

   Cui et al Sci Rep. 2017 Apr 24;7:46392. doi: 10.10

   Insert

   KANK1 (KANK1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   CMV promoter

   Available Since

   Feb. 28, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAS_4xBstTyrT(CUA)_EF1 sfGFP150TAG

    Plasmid

    #174891

    Purpose

    amber suppression reporter sfGFP150TAG expression, with BstTyr(CUA) amber suppressor tRNA cassette

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    150TAG in sfGFP

    Promoter

    EF1

    Available Since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. PXPR007 sgGFP-1

    Plasmid

    #74960

    Purpose

    Cas9 + sgGFP-1 with blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    GFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Bxb1_miRFP703

    Plasmid

    #183759

    Purpose

    Bxb1-specific donor plasmid for intergrating a transcription unit for expression of membrane-localised miRFP703

    Depositor

    Richard Davis

    Article

    Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

    Insert

    miRFP703
    

    Use

    Tags

    Lck lipidation signal

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. GB1-His-TET1-848-952(pL91)

    Plasmid

    #124388

    Purpose

    E.Coli produced TET1-848-952 purified by FPLC nickel column

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    GB1-His-TET1-848-952 wildtype (TET1 Human)
    

    Use

    Tags

    GB1-Histidine

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPV-H1-ccdB-mEF1α-RiH

    Plasmid

    #100598

    Purpose

    piggyBac vector for sgRNA cloning

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Type

    Empty backbone

    Use

    Piggybac vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human H1 PolIII promoter, human FTH1 promoter, mo…

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA

    Plasmid

    #220224

    Purpose

    AAV-STARR-seq validation control vector with Venus

    Depositor

    Simon Rumpel

    Article

    Chan et al Sci Rep. 2023 Apr 25;13(1):6745. doi: 10

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGL4.10-VEGFprom(-500 to -1)

    Plasmid

    #66130

    Purpose

    Luciferase-based reporter for VEGF promoter region (-500 to -1 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor-A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    VEGF -500 to -1

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSCALPS-mAgo2-EGFP

    Plasmid

    #209935

    Purpose

    Lentiviral expression of mouse Argonaute-2 in mammalian cells

    Depositor

    Phillip Zamore

    Article

    Jouravleva et al Cell Rep Methods. 2022 Mar 18;2(3):10018

    Insert

    Ago2 (Ago2 Mouse)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pME18ST-d5-HT7

    Plasmid

    #53111

    Purpose

    Expression of Drosophila 5-HT7

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT7 (5-HT7 Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

19. pAAV-EnhancedSynapsin-DIO-TVA950-EYFP-WPRE

    Plasmid

    #120269

    Purpose

    Allows for initial cellular transduction of EnvA-pseudotyped Rb via Cre-dependent expression of an avian TVA receptor fused with yellow fluorescent protein

    Depositor

    Edward Callaway

    Article

    Faget et al Cell Rep. 2016 Jun 21;15(12):2796-808. d

    Insert

    eSyn-DIO-TVA950:YFP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSico-shRaptor

    Plasmid

    #81086

    Purpose

    conditional expression of an shRNA targeting murine Raptor

    Depositor

    Angelique Bordey

    Article

    Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

    Insert

    Raptor shRNA (Rptor Mouse)
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Sept. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGL4.10-VEGFprom(-1000 to -500)

    Plasmid

    #66129

    Purpose

    Luciferase-based reporter for VEGF promoter region (-1000 to -500 bp)

    Depositor

    David Mu

    Article

    Wood et al Sci Rep. 2016 Feb 25;6:19857. doi: 10.10

    Insert

    Vascular Endothelial Growth Factor -A promoter region (VEGFA Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    VEGF -500 to -1

    Available Since

    March 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pME18ST-d5-HT2A

    Plasmid

    #53109

    Purpose

    Expression of Drosophila 5-HT2A

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT2A (5-HT2 Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pME18ST-d5-HT1A

    Plasmid

    #53107

    Purpose

    Expression of Drosophila 5-HT1A

    Depositor

    Leslie Vosshall

    Article

    Gasque et al Sci Rep. 2013;3:srep02120. doi: 10.1038/

    Insert

    5HT1A (5-HT1A Fly)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 24, 2014

    Availability

    Academic Institutions and Nonprofits only

24. RT3GEN

    Plasmid

    #111165

    Purpose

    Tet-regulated miR-E (miR-30 variant)-based RNAi with tGFP reporter

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    T3G

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. mCherry-PH-SWAP70

    Plasmid

    #84582

    Purpose

    Expresses mCherry-tagged PH-domain of SWAP70 in mammalian cells, binds to PI(3,4)P2

    Depositor

    Geert van den Bogaart

    Article

    Baranov et al Cell Rep. 2016 Nov 1;17(6):1518-1531. do

    Insert

    SWAP70 residues 209-306 (SWAP70 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Pleckstrin-homology domain, residues 209-306

    Promoter

    CMV

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

26. pK098.CAG-loxP-stop-loxP-nlstagRFP-ires-tTA-WPRE (Supernova)

    Plasmid

    #85179

    Purpose

    For nucleus-specific RFP labeling in single cells, pK098 should be used with pK031.

    Depositor

    Takuji Iwasato

    Article

    Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

    Insert

    nuclear localization signal (nls) tagRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FgH1tUTG_huBim_Ex3

    Plasmid

    #85532

    Purpose

    Inducible expression of guide RNA (huBim_Ex3) with fluorescent GFP reporter

    Depositor

    Marco Herold

    Article

    Aubrey et al Cell Rep. 2015 Mar 3;10(8):1422-32. doi:

    Insert

    hu Bim
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1t

    Available Since

    Jan. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCX-SponGee-Kras

    Plasmid

    #134777

    Purpose

    mammalian expression of SponGee-Kras, a plasma membrane-targeted but lipid raft-excluded variant of the cGMP buffer SponGee that prevents downstream signaling

    Depositor

    Xavier Nicol

    Article

    Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

    Insert

    SponGee-Kras
    

    Use

    Tags

    FLAG and mRFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Jan. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. NG0886 pCI-TPI-WT-4H

    Plasmid

    #65801

    Purpose

    NMD reporter mRNA

    Depositor

    Niels Gehring

    Article

    Boehm et al Cell Rep. 2014 Oct 23;9(2):555-68. doi:

    Insert

    TPI (TPI1 Human)
    

    Use

    Tags

    4H (4 copies of short beta-globin 3'UTR for …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAG-GFP-PQRv3-RFP

    Plasmid

    #73951

    Purpose

    Protein Quantitation Reporter (PQR) to quantify a protein of interest in mammalian cells.

    Depositor

    Brian Chen

    Article

    Lo et al Cell Rep. 2015 Dec 22;13(11):2634-44. do

    Inserts

    sfGFP (superfolder GFP)
    RFP
    

    Use

    Tags

    A residual PQR peptide wii be left at the C-termi…

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken beta actin and Chicken beta actin (shared…

    Available Since

    Jan. 6, 2017

    Availability

    Academic Institutions and Nonprofits only