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pBabe-TetCMV-puro-mVenus-PA-Rac1

Plasmid

#22008

Purpose

Mammalian expression of photoactivatable Rac1

Depositor

Klaus Hahn

Publication

Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

Insert

PA-Rac1 (RAC1 Human, Avena sativa (oat))

Use

Retroviral

Tags

mVenus

Expression

Mammalian

Mutation

LOV(Leu404-Leu546), Rac1 starts at I4 and contain…

Available since

Sept. 29, 2009

Availability

Academic Institutions and Nonprofits only
pBCN21-R4R3

Plasmid

#26301

Depositor

Ben Lehner

Publication

Semple et al Nat Methods. 2010 Sep;7(9):725-7. doi: 1

Type

Empty backbone

Expression

Worm

Available since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pJH200

Plasmid

#13327

Depositor

Martin Gellert

Publication

Lieber et al Genes Dev. 1987 Oct . 1(8):751-61.

Insert

recombination substrate

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pEF-Bos TRAM Flag

Plasmid

#41551

Depositor

Kate Fitzgerald, Doug Golenbock

Publication

Fitzgerald et al J Exp Med. 2003 Oct 6;198(7):1043-55. Ep

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TRAM (TICAM2 Human)

Tags

6xHis and Flag

Expression

Mammalian

Promoter

EF1a

Available since

Dec. 14, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pMAL-XPG

Plasmid

#39215

Depositor

Aziz Sancar

Publication

Matsunaga et al J Biol Chem. 1995 Sep 1;270(35):20862-9.

Insert

XPG (ERCC5 Human)

Tags

MBP

Expression

Bacterial

Mutation

Note that XPG starts at amino acid 5

Promoter

Ptac

Available since

Aug. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pQE31-rsEGFP2

Plasmid

#102879

Purpose

Expresses rsEGFP2 in bacterial cells

Depositor

Stefan Jakobs

Publication

Grotjohann et al eLife. 2012 Dec 31:1:e00248. doi: 10.755

Insert

rsEGFP2

Tags

6xHisTag

Expression

Bacterial

Available since

Jan. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHR_MFE1_hrGFP

Plasmid

#84617

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into MFE1 locus

Depositor

Ian Wheeldon

Publication

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pDONR223-GSG2

Plasmid

#23472

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

GSG2 (HASPIN Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pMSCVhyg-H3.3WT-FLAG/HA

Plasmid

#128740

Purpose

Expression of histone H3.3WT-FLAG/HA

Depositor

Kai Ge

Publication

Jang et al Nucleic Acids Res. 2019 Jan 25;47(2):607

Insert

H3.3 WT (H3-3A Human)

Use

Retroviral

Tags

FLAG and HA

Promoter

MSCV

Available since

Aug. 8, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y,he:tagBFP2

Plasmid

#158651

Purpose

making transgenics expressing a UAS driven YFP and NTR 2.0

Depositor

Jeff Mumm

Publication

Sharrock et al Nat Methods. 2022 Feb;19(2):205-215. doi

Insert

5xUAS:GAP-tagYFP-P2A-NfsB_Vv F70A/F108Y;he:BFP

Use

Tol2-based transgenic expression in zebrafish/oth…

Mutation

F70A/F108Y

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pcDNA5-Flag-BRD4-7A

Plasmid

#90006

Purpose

expresses 7A mutant BRD4

Depositor

Kornelia Polyak

Publication

Shu et al Nature. 2016 Jan 21;529(7586):413-7. doi

Insert

BRD4 (BRD4 Human)

Tags

Flag

Expression

Mammalian

Mutation

Changed 484S to 484A, 488S to 488A, 492S to 492A,…

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pINDUCER20 MEF2C

Plasmid

#89717

Purpose

Lentiviral gene expression vector for doxycycline inducible MEF2C expression

Depositor

Alex Kentsis

Publication

Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Expression

Mammalian

Available since

May 23, 2017

Availability

Academic Institutions and Nonprofits only

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GUT BuGZ FL

Plasmid

#84024

Purpose

Expression of GFP tagged BuGZ in human cells

Depositor

Patrick Paddison

Publication

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BuGZ (ZNF207 Human)

Use

Lentiviral

Tags

TurboGFP

Expression

Mammalian

Mutation

Q394R

Promoter

UCOE / SFFV

Available since

Oct. 28, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pGEMABC

Plasmid

#45398

Depositor

Katsu Murakami

Publication

Murakami et al J Biol Chem. 2013 Mar 29;288(13):9126-34

Inserts

rpoA (rpoA Escherichia coli)
rpoB (rpoB Escherichia coli)
rpoC (rpoC Escherichia coli)

Expression

Bacterial

Promoter

None and T7

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCW57-MCS1-P2A-MCS2-mDux

Plasmid

#138320

Purpose

Dox-inducible codon-optimized mouse Dux

Depositor

Yi Zhang

Publication

Fu et al Nat Cell Biol. 2019 Jul;21(7):835-844. d

Insert

Dux (Dux Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

TRE

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLKO5d.EFS.SpCas9.P2A.PAC

Plasmid

#58329

Purpose

Lentiviral Vector for SpCas9 Expression without sgRNA, Puromycin resistance, EFS Promoter driven

Depositor

Benjamin Ebert

Publication

Heckl et al Nat Biotechnol. 2014 Jun 22. doi: 10.103

Inserts

SpCas9
EFS (EEF1A1 Human)
P2A-PAC

Use

CRISPR and Lentiviral

Tags

FLAG

Available since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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FRP2370

Plasmid

#127576

Purpose

Repressor plasmid for WTC 846. Encodes TetR and TetR-Tup1, Leu2MX selection marker

Depositor

Joerg Stelling

Publication

Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

Insert

PRnr2_TetR-NLS-TUP1_tCyc1_PtetO7.1_TetR-NLS_tCyc1

Use

Synthetic Biology; Shuttle vector

Expression

Yeast

Available since

Aug. 13, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDONR223-PGK1

Plasmid

#23427

Depositor

William Hahn, David Root

Publication

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PGK1 (PGK1 Human)

Use

Gateway Cloning

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pCag FlpO-2A-Cre EV

Plasmid

#129419

Purpose

episomal expression of FlpO and Cre recombinases

Depositor

Joshua Breunig

Publication

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

FlpO-2A-Cre

Use

Cre/Lox and Unspecified; Episomal expression vect…

Expression

Mammalian

Promoter

CMV/Chick β-actin (CAG)

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pTRV2-GG

Plasmid

#105349

Purpose

virus-induced gene silencing

Depositor

Johannes Stuttmann

Publication

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

TRV-RNA2(5')

Use

In planta gene silencing; viral

Mutation

BsaI restriction sites removed

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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