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We narrowed to 12,055 results for: cel.2

Showing: 6701 - 6750 of 12055 results

  1. GFP Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pcDNA3

    Plasmid
    #206093
    Purpose
    Cav2.2 calcium channel with a N-terminal GFP tag, exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206093-plasmid-map-sequence-id-415677

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  2. Cav2.2 HA EI,II,III,IVA (E314A, E664A, E1370A, E1658A) pCAGGS

    Plasmid
    #206098
    Purpose
    expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and mutations in the P loop of Domains I, II, III and IV which abolish trafficking to the cell surface
    Depositor
    Annette Dolphin
    Article
    Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi
    Insert
    cacna1b (CACNA1B Rabbit)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    E314A, E664A, E1370A, E1658A
    Promoter
    CMV/B-actin
    Available Since
    Nov. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    206098-plasmid-map-sequence-id-415670

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  3. pGL3-noSV40-humanEC1.45_p300peak1-2_7XCoordinatorMutant

    Plasmid
    #173994
    Purpose
    Firefly luciferase enhancer reporter plasmid.
    Depositor
    Joanna Wysocka
    Article
    Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783
    Insert
    p300 peak 1 and peak 2 from SOX9 enhancer cluster EC1.45 with all 7 Coordinator motifs mutated
    Use
    Luciferase
    Tags
    Expression
    Mutation
    7X Coordinator motif mutation #1-2-3-4-5-6-7
    Promoter
    SV40 promoter
    Available Since
    Sept. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    173994-plasmid-map-sequence-id-341795

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  13. pPapi

    Plasmid
    #96921
    Purpose
    expression of S aureus SaCas9 (SauCas9) and two pol III promoters for an Sa gRNA and an Sp gRNA. Intended to be used in SpCas9-expressing cell lines for dual Cas9 "Big Papi" screens. aka pXPR207.
    Depositor
    David Root, John Doench
    Article
    Najm et al Nat Biotechnol. 2018 Feb;36(2):179-189.
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available Since
    June 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    96921-plasmid-map-sequence-id-181715

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  16. GFP-PHIP

    Plasmid
    #65389
    Purpose
    GFP-tagged BRD protein, which can be used to be expressed in mammalian cells.
    Depositor
    Kyle Miller
    Article
    Gong et al Genes Dev. 2015 Jan 15;29(2):197-211. do
    Insert
    PHIP (PHIP Human)
    Use
    Tags
    EmGFP and V5
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    May 14, 2015
    Availability
    Academic Institutions and Nonprofits only

  23. pCI-Flag-NEDD4L∆WW1,2,4

    Plasmid
    #171137
    Purpose
    expresses NEDD4L with inactivating mutation in first, second and fourth WW domain, but intact third WW domain, in mammalian cells
    Depositor
    Wesley Sundquist
    Article
    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10
    Insert
    NEDD4L (NEDD4L Human)
    Use
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    changed tryptophan 221 to alanine, tryptophan 453…
    Promoter
    Available Since
    July 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    171137-plasmid-map-sequence-id-338242

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  25. HA-IFITM3-F8-TAG-C72A

    Plasmid
    #122477
    Purpose
    expresses human IFITM3-C72A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Promoter
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122477-plasmid-map-sequence-id-237728

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  27. IFITM3-N21del-V93-TAG

    Plasmid
    #122480
    Purpose
    expresses human IFITM3-N21del with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon and deleted amin…
    Promoter
    Available Since
    March 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    122480-plasmid-map-sequence-id-237764

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  29. HA-IFITM3-F8-TAG-C105A

    Plasmid
    #122478
    Purpose
    expresses human IFITM3-C105A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Promoter
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122478-plasmid-map-sequence-id-237731

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  30. HA-IFITM3-V93-TAG

    Plasmid
    #122479
    Purpose
    expresses human IFITM3 with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Valine 93 to amber codon
    Promoter
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122479-plasmid-map-sequence-id-237724

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  31. HA-IFITM3-F8-TAG-C71A

    Plasmid
    #122476
    Purpose
    expresses human IFITM3-C71A with unnatural amino acid incorporated site specifically in response to the amber codon TAG in mammalian cells when co-transfected with tRNA synthetase/tRNA pair.
    Depositor
    Howard Hang
    Article
    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038
    Insert
    IFITM3 (IFITM3 Human)
    Use
    Tags
    HA
    Expression
    Mammalian
    Mutation
    Changed Phenylalanine 8 to amber codon, cysteine …
    Promoter
    Available Since
    March 8, 2019
    Availability
    Academic Institutions and Nonprofits only
    122476-plasmid-map-sequence-id-237722

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  35. VIPR2-DuET

    Plasmid
    #213387
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    VIPR2 (VIPR2 Human)
    Use
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213387-plasmid-map-sequence-id-430740

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  45. pBig1a zz TEV YBBR POT1 MBP TEV TPP1 MBP TEV TIN2 ZZ TEV TRF2 (4comp2)

    Plasmid
    #185448
    Purpose
    Coexpresses human POT1 with a zz affinity tag, YBBR site, and TEV site, human TRF2 with a zz tag and TEV site, and human TIN2 and TPP1 each with an MBP affinity tag and TEV site in insect cells
    Depositor
    Ahmet Yildiz
    Article
    Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9.
    Inserts
    TRF2 (TERF2 Human)
    POT1 (POT1 Human)
    TPP1 (TPP1 Human)
    TIN2 (TINF2 Human)
    Use
    Tags
    MBP, YBBR, and ZZ
    Expression
    Insect
    Mutation
    See Depositor Comments Below
    Promoter
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    185448-plasmid-map-sequence-id-364976

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  46. pX330-U6-(negative selection module)-Chimeric_BB-CBh-hSpCas9

    Plasmid
    #223321
    Purpose
    It contains a ccdB-CMr negative selection module flanked by BpiI (BbsI) sites that allows excluding clones without correct insertion of sgRNA. Human codon optimized Cas9 expressing plasmid.
    Depositor
    Jonathan Arias
    Article
    Petruskeviciute et al Stem Cell Res. 2024 Dec 12;82:103628. do
    Inserts
    SpCas9 codon optimized for human (cas9 Human, Synthetic)
    chloramphenicol resistance protein
    CcdB
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available Since
    Dec. 13, 2024
    Availability
    Academic Institutions and Nonprofits only
    223321-plasmid-map-sequence-id-447743

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  47. pCS2-NES-Myc(340-439)-GFP-IRES-mCherry (NES-DBD)

    Plasmid
    #231237
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NES for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NES fused to the DNA-binding-domain of MYC (resid…
    Promoter
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231237-plasmid-map-sequence-id-459172

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  48. pCS2-MYC(Δ1-143; Δ340-439)-GFP-IRES-mCherry (ΔTADΔDBD)

    Plasmid
    #231235
    Purpose
    MYC stability reporter construct with deletion of the N-terminal region (residues 1-143) and DNA-binding-domain (residues 340-439) for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    TAD deleted (delta1-143), DBD deleted (delta340-4…
    Promoter
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231235-plasmid-map-sequence-id-459171

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  49. pCS2-NLS-Myc(340-439)-GFP-IRES-mCherry (NLS-DBD)

    Plasmid
    #231236
    Purpose
    MYC stability reporter construct of the DNA-binding-domain (residues 340-439) fused to an N-terminal NLS for transient expression in mammalian cells
    Depositor
    Michael Rape
    Article
    Mark et al Cell. 2023 Aug 3;186(16):3460-3475.e23.
    Insert
    MYC (MYC Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    NLS fused to the DNA-binding-domain of MYC (resid…
    Promoter
    Available Since
    Jan. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    231236-plasmid-map-sequence-id-459169

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Showing: 6701 - 6750 of 12055 results