Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

Filter By

We narrowed to 11,711 results for: pcDNA

Showing: 6701 - 6750 of 11711 results

  5. pcDNA3.1-N-Myc_DED/AAA 535-888 USP7

    Plasmid
    #131255
    Purpose
    Mammalian expression of USP7 UBL domains 1-3, with an N-terminal Myc tag and mutations in UBL2 (corresponding to DE758AA_D764A of the full-length protein)
    Depositor
    Roger Woodgate
    Article
    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d
    Insert
    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    Use
    Tags
    Myc
    Expression
    Mammalian
    Mutation
    A mutant form of our pcDNA3.1-N-Myc_535-888 USP7 …
    Promoter
    Available since
    Dec. 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    131255-plasmid-map-sequence-id-267709

    Enlarge

  6. pcDNA-GFP-EBA175-Flag(M1423R/P1424V)

    Plasmid
    #155309
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 M1423R/P1424V variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    M1423R/P1424V; insert codes for EBA175 residues 1…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155309-plasmid-map-sequence-id-310078

    Enlarge

  7. pcDNA-GFP-EBA175-Flag(P1424V/Y1425W)

    Plasmid
    #155310
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1425W variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424V/Y1425W; insert codes for EBA175 residues 1…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155310-plasmid-map-sequence-id-309982

    Enlarge

  8. pcDNA-GFP-EBA175-Flag(P1424V/Y1426H)

    Plasmid
    #155311
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424V/Y1426H variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424V/Y1426H; insert codes for EBA175 residues 1…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155311-plasmid-map-sequence-id-309989

    Enlarge

  9. pcDNA-GFP-EBA175-Flag(P1424Q/Y1426S)

    Plasmid
    #155003
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424Q/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424Q/Y1426S; insert codes for EBA175 residues 1…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155003-plasmid-map-sequence-id-309847

    Enlarge

  10. pcDNA-GFP-EBA175-Flag(P1424R/Y1426S)

    Plasmid
    #155004
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424R/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424R/Y1426S; insert codes for EBA175 residues 1…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155004-plasmid-map-sequence-id-309980

    Enlarge

  11. pcDNA-GFP-EBA175-Flag(P1424D/Y1426S)

    Plasmid
    #155005
    Purpose
    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EBA175 P1424D/Y1426S variant (residues 1284-1462) from pcDNA3.1
    Depositor
    Sinisa Urban
    Article
    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-
    Insert
    PfEBA175
    Use
    Tags
    Flag, GFP, and signal peptide (residues 1 to 32) …
    Expression
    Mammalian
    Mutation
    P1424D/Y1426S; insert codes for EBA175 residues 1…
    Promoter
    CMV
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    155005-plasmid-map-sequence-id-310099

    Enlarge

  12. pcDNA3.1-CMV-LMO2 (GLuc-VChR1-EYFP)

    Plasmid
    #72889
    Purpose
    fusion protein of Gaussia luciferase, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics
    Depositor
    Ute Hochgeschwender
    Article
    Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371
    Inserts
    Gaussia luciferase, Volvox channelrhodopsin 1, EYFP
    Volvox channelrhodopsin 1
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Aug. 27, 2019
    Availability
    Academic Institutions and Nonprofits only
    72889-plasmid-map-sequence-id-252489

    Enlarge

  14. FLAG-MBP1-E322TAG-E309C-W340A-CAAX.pcDNA3-k

    Plasmid
    #127409
    Purpose
    Expresses MBP in mammalian cells. Amber codon at AA# 322 as a noncanonical amino acid incorporation site
    Depositor
    Sharona Gordon
    Article
    Gordon et al Elife. 2018 Jun 20;7. pii: 37248. doi: 1
    Insert
    Maltose Binding Protein
    Use
    Tags
    CAAX and FLAG
    Expression
    Mammalian
    Mutation
    Amino acid mutations: E322 to Amber codon, E309C …
    Promoter
    CMV
    Available since
    July 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    127409-plasmid-map-sequence-id-248721

    Enlarge

  31. pcDNA3/ TO myc-cCE-bio (cCE-bio)

    Plasmid
    #82473
    Purpose
    Expresses cytoplasmic restricted form of N-terminal myc tagged and C-terminal biotin tagged mRNA capping enzyme, inactive form
    Depositor
    Daniel Schoenberg
    Article
    Mukherjee et al PLoS Biol. 2014 Aug 19;12(8):e1001933. d
    Insert
    Myc-NES-mCE (Wt, NLS deletion)-TEV-Bio (Rngtt Synthetic, Mouse)
    Use
    Tags
    TEV-Biotin and myc
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
    82473-plasmid-map-sequence-id-163076

    Enlarge

  33. CFP(159-238)Stop in pcDNA1/amp

    Plasmid
    #60893
    Purpose
    This vector attached CFP(159-238) to the C-terminus of a protein.
    Depositor
    Catherine Berlot
    Insert
    CFP(159-238)Stop
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Asn-164 was changed to His
    Promoter
    CMV
    Available since
    Aug. 4, 2015
    Availability
    Academic Institutions and Nonprofits only
    60893-plasmid-map-sequence-id-118771

    Enlarge

  34. Cer(159-238)-beta-5 in pcDNAI/Amp

    Plasmid
    #55778
    Purpose
    A carboxyl-terminal mCerulean fragment was fused to Gbeta-5. When co-expressed with an amino terminal mCerulean fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    Cer(159-238)-beta-5 (GNB5 Aequorea victoria, Human)
    Use
    Tags
    mCer(159-238) was fused to the amino terminus of …
    Expression
    Mammalian
    Mutation
    GBeta-5 was amplified via PCR, which added an N-t…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55778-plasmid-map-sequence-id-114869

    Enlarge

  35. YFP(159-238)-gamma-5 in pcDNAI/Amp

    Plasmid
    #55191
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-5. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    YFP(159-238)-gamma-5 (GNG5 Bovine, Aequorea victoria)
    Use
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-5 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55191-plasmid-map-sequence-id-115046

    Enlarge

  36. YFP(159-238)-gamma-11 in pcDNAI/Amp

    Plasmid
    #55193
    Purpose
    A carboxyl-terminal YFP fragment was fused to Ggamma-11. When co-expressed with an amino-terminal YFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    YFP(159-238)-gamma-11 (GNG11 Bovine, Aequorea victoria)
    Use
    Tags
    YFP(159-238) was fused to the amino terminus of G…
    Expression
    Mammalian
    Mutation
    Ggamma-11 was amplified by PCR, which added a Bam…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55193-plasmid-map-sequence-id-115059

    Enlarge

  41. pcdna3 T7 Jip1b(471-707)(C478S and C699T)

    Plasmid
    #52132
    Purpose
    a mammalian scaffold protein that mediates the activation of a MAP kinase signaling pathway.
    Depositor
    Roger Davis
    Article
    Whitmarsh et al Science. 1998 Sep 11;281(5383):1671-4.
    Insert
    JNK interacting protein-1b (JIP-1b)(471-707)(M1+6 ) (Mapk8ip1 Mouse)
    Use
    Tags
    T7
    Expression
    Mammalian
    Mutation
    deleted amino acid 1-470 and changed C478S and C6…
    Promoter
    Available since
    June 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  44. pcDNA3.1 Flag YFP hNKCC1 V729C (NT503)

    Plasmid
    #50883
    Purpose
    Expresses human NKCC1 V729C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    V729C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 24, 2014
    Availability
    Academic Institutions and Nonprofits only
    50883-plasmid-map-sequence-id-76356

    Enlarge

  45. pcDNA3.1 Flag YFP hNKCC1 Y739C (NT525)

    Plasmid
    #50893
    Purpose
    Expresses human NKCC1 Y739C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    Y739C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 13, 2014
    Availability
    Academic Institutions and Nonprofits only
    50893-plasmid-map-sequence-id-76366

    Enlarge

  46. pcDNA3.1 Flag YFP hNKCC1 C723S (NT537)

    Plasmid
    #50902
    Purpose
    Expresses human NKCC1 C723S mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    C723S in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50902-plasmid-map-sequence-id-76375

    Enlarge

  47. pcDNA3.1 Flag YFP hNKCC1 I674C (NT475)

    Plasmid
    #50875
    Purpose
    Expresses human NKCC1 I674C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    I674C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50875-plasmid-map-sequence-id-76348

    Enlarge

  48. pcDNA3.1 Flag YFP hNKCC1 V740C (NT526)

    Plasmid
    #50894
    Purpose
    Expresses human NKCC1 V740C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    V740C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50894-plasmid-map-sequence-id-76367

    Enlarge

  49. pcDNA3.1 Flag YFP hNKCC1 L737C (NT523)

    Plasmid
    #50891
    Purpose
    Expresses human NKCC1 L737C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Article
    Monette et al J Biol Chem. 2014 Jan 22.
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    L737C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50891-plasmid-map-sequence-id-76364

    Enlarge

  50. pcDNA3.1 Flag YFP hNKCC1 T738C (NT524)

    Plasmid
    #50892
    Purpose
    Expresses human NKCC1 T738C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.
    Depositor
    Biff Forbush
    Insert
    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    Use
    Tags
    3x Flag and YFP
    Expression
    Mammalian
    Mutation
    T738C in hNKCC1 in NT17
    Promoter
    CMV
    Available since
    Feb. 6, 2014
    Availability
    Academic Institutions and Nonprofits only
    50892-plasmid-map-sequence-id-76365

    Enlarge

Showing: 6701 - 6750 of 11711 results