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We narrowed to 6,268 results for: kit

Showing: 5401 - 5440 of 6268 results
  1. 3xFLAG-dCas9/MSCV-EGFP

    Plasmid
    #82613
    Purpose
    Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.
    Depositor
    Insert
    3xFLAG-dCas9
    Use
    Retroviral
    Tags
    3xFLAG tag and NLS (nuclear localization signal)
    Expression
    Mutation
    human codon-optimized, D10A + H840A
    Promoter
    LTR
    Available since
    Sept. 16, 2016
    Availability
    Academic Institutions and Nonprofits only
  2. pCAG MCP-VP64

    Plasmid
    #92357
    Purpose
    CAG-driven ubiquitous expression of MCP-VP64. MCP is bacteriophage MS2 coat protein, VP64 is transcriptional activation domain. Part of SAM-mediated gene activation system in chicken embryos.
    Insert
    MCP-VP64
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
  3. pMOD_B2312

    Plasmid
    #91070
    Purpose
    Module B, Promoter: PvUbi1, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    PvUbi1
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  4. pDIRECT_26H

    Plasmid
    #91150
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:bar
    Depositor
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  5. pDONR P2R-P3-mKate2

    Plasmid
    #48345
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 3’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Insert
    mKate2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a stop codon at the 3' end of the c…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  6. pDONR P4-P1R-EYFP

    Plasmid
    #48350
    Purpose
    Contributes the coding sequence for EYFP as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Insert
    EYFP
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Contains a Kozak sequence. Does not contain a sto…
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  7. pMOD_C2616

    Plasmid
    #91090
    Purpose
    Module C, Promoter: At7SL, Gene: BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Insert
    BsaI ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    At7SL
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  8. pMOD_B2515b

    Plasmid
    #91073
    Purpose
    Module B, Promoter: AtU6, Gene: Esp3I ccdb cassette for single gRNA spacer cloning, structurally optimized gRNA scaffold, Terminator: Pol III
    Depositor
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    AtU6
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  9. pMOD_B2301

    Plasmid
    #91067
    Purpose
    Module B, Promoter: 35S, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    35S
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  10. pMOD_C2517

    Plasmid
    #91086
    Purpose
    Module C, Promoter: TaU3, Gene: Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning), Terminator: Pol III
    Depositor
    Insert
    Esp3I ccdb cassette for single gRNA spacer cloning (+ BaeI site for GT donor cloning)
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    TaU3
    Available since
    July 14, 2017
    Availability
    Academic Institutions and Nonprofits only
  11. pAH-CTX1-rhadCas9-native

    Plasmid
    #129392
    Purpose
    Derived from pAH-CTX1-rha. The native Streptococcus pyogenes dCas9 gene cloned downstream of PrhaBAD. Non-codon-optimized dCas9 version of pAH-CTX1-rhadCas9.
    Depositor
    Insert
    S. pyogenes dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Aspartate 10 to Alanine, Histidine 840 to Alanine
    Promoter
    PrhaBAD
    Available since
    Dec. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
  12. pAH18

    Plasmid
    #154397
    Purpose
    Derived from pGPI-SceI; Trimethoprim resistant; carries I-SceI gene; contains 475 bp from both upstream and downstream flanking regions between the FRT sites on pAHCTX1-rhadCas9
    Depositor
    Insert
    Fused upstream and downstream homologous regions around FRT sites when pAH-CTX1-rhadCas9 integrated into K56-2 genome
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    NA
    Available since
    Aug. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
  13. pMOD_B2309

    Plasmid
    #91069
    Purpose
    Module B, Promoter: YAO, Gene: SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers , Terminator: 35S
    Depositor
    Insert
    SapI ccdb cassette for cloning multiple gRNA spacers with tRNA spacers
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    YAO
    Available since
    July 13, 2017
    Availability
    Academic Institutions and Nonprofits only
  14. pJG367

    Plasmid
    #91185
    Purpose
    BeYDV replicon T-DNA for gene targeting in tobacco leaves, H840A double nickase (nAtCas9_H840A+gNt_R2+gNt_F2+donor)
    Depositor
    Insert
    nAtCas9_H840A+gNt_R2+gNt_F2+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  15. pJG382

    Plasmid
    #91189
    Purpose
    BeYDV replicon T-DNA for gene targeting in tobacco leaves, D10A double nickase (nAtCas9_D10A+gNt_R2+gNt_F2+donor)
    Depositor
    Insert
    nAtCas9_D10A+gNt_R2+gNt_F2+donor
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
  16. JPF0419d

    Plasmid
    #124020
    Purpose
    Encodes pCAG driving expression of multicistronic Lyn-tagged iRFP713, cytoplasmic mAzamiGreen, mCerulean-tethered p38 KTR, and Histone 2B fused to mScarlet in a PiggyBac destination vector
    Depositor
    Insert
    PB_pCAG-Lyn-iRFP713-P2A-NES-mAzamiGreen-P2A-p38KTR-mCerulean-P2A-H2B::mScarlet
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 13, 2019
    Availability
    Academic Institutions and Nonprofits only
  17. pDONR P4-P1R-mKate2

    Plasmid
    #48344
    Purpose
    Contributes the coding sequence for the mKate2 fluorescent protein as the 5’-module during MultiSite Gateway-cloning of chimeric cDNAs encoding three-part fusion proteins.
    Insert
    mKate2
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    It contains a Kozak sequence but no stop codon.
    Promoter
    none
    Available since
    April 9, 2014
    Availability
    Academic Institutions and Nonprofits only
  18. pDIRECT_21B

    Plasmid
    #91129
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA, Plant Selection: 2x35S:hpt II
    Depositor
    Insert
    Engineering Reagent: 35S:AtCas9_dead + AtU6:gRNA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
  19. pDIRECT_26G

    Plasmid
    #91149
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:bar
    Depositor
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
  20. pDIRECT_25G

    Plasmid
    #91144
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA , Plant Selection: PvUbi2:hpt II
    Depositor
    Insert
    Engineering Reagent: ZmUbi:TaCas9_dead + TaU6:gRNA
    Use
    CRISPR
    Tags
    Expression
    Plant
    Mutation
    D10A, H840A
    Promoter
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
Showing: 5401 - 5440 of 6268 results