We narrowed to 7,692 results for: Lif;
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Plasmid#112810PurposePCR template vector for amplifying gRNAcore-pU6.2 promoter fragmentDepositorInsertU6:3-gRNAcore
ExpressionBacterialAvailable SinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX1ZFCNGA5
Plasmid#69859PurposeGST tagged protein for antibody production screeningDepositorAvailable SinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestMYC_RBPMS2
Plasmid#65668PurposeMYC expression vector with RBPMS2DepositorInsertRBPMS2 (RBPMS2 Human)
TagsMYCExpressionMammalianMutationintroduced HindIII site to exchange FLAG-HA tag w…PromoterCMV, 2x TetAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_R38E
Plasmid#65665PurposeFLAG-HA expression vector with RBPMS carrying mutation R38EDepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged arginine 38 to glutamic acidPromoterCMV, 2x TetAvailable SinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_R38A_E39A
Plasmid#65664PurposeFLAG-HA expression vector with RBPMS carrying mutation R38A, E39ADepositorInsertRBPMS (RBPMS Human)
TagsFLAG HAExpressionMammalianMutationchanged arginine 38 to alanine, and glutamic acid…PromoterCMV, 2x TetAvailable SinceJune 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 A720C C723S C724V (NT534)
Plasmid#50899PurposeExpresses human NKCC1 A720C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationA720C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 L718C C723S C724V (NT532)
Plasmid#50897PurposeExpresses human NKCC1 L718C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationL718C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 L722C C723S C724V (NT536)
Plasmid#50901PurposeExpresses human NKCC1 L722C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationL722C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 S716C C723S C724V (NT530)
Plasmid#50895PurposeExpresses human NKCC1 S716C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationS716C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 I721C C723S C724V (NT535)
Plasmid#50900PurposeExpresses human NKCC1 I721C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationI721C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V W732C (NT546)
Plasmid#50908PurposeExpresses human NKCC1 C723S C724V W732C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationC723S C724V W732C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V I725C (NT538)
Plasmid#50903PurposeExpresses human NKCC1 C723S C724V I725C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationC722S C723V I724C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 C723S C724V V726C (NT539)
Plasmid#50904PurposeExpresses human NKCC1 C723S C724V V726C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationC723S C724V V726C in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 G719C C723S C724V (NT533)
Plasmid#50898PurposeExpresses human NKCC1 G719C C723S C724V mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and YFPExpressionMammalianMutationG719C C723S C724V in hNKCC1 in NT17PromoterCMVAvailable SinceFeb. 6, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 Flag YFP hNKCC1 N-terminal deletion (NT53)
Plasmid#49062PurposeExpresses human NKCC1 truncation mutant (aa13-222) with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.DepositorInserthuman NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
Tags3x Flag and mVenusExpressionMammalianMutationN-terminal deletion of amino acids 13-222 in hNKC…PromoterCMVAvailable SinceNov. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARδ
Plasmid#119916PurposeMammalian expression of the tAKARδ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARδ
ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
pRK5_tAKARε
Plasmid#119917PurposeMammalian expression of the tAKARε sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)DepositorInserttAKARε
ExpressionMammalianAvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xHA-hTERT
Plasmid#51637PurposeMammalian expression of 3xHA tagged human TERT, N-terminal.DepositorAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAGM9037
Plasmid#153225PurposeTemplate for PCR amplification of the constant part of guide RNAsDepositorInsertguide RNA constant part, carotenoid marker
UseSynthetic BiologyAvailable SinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSUMO His6 SUMO MYC3
Plasmid#177848PurposeBacterial Expression of MYC3DepositorAvailable SinceJan. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGG-cTP-TurboID-GFP
Plasmid#209403PurposeTransiently expressing and visualizing the subcellular localization of TurboID fused with a chloroplast transit peptide (cTP) in plantaDepositorInsertPM-3XHA-TurbOID
TagsGFPExpressionPlantPromoterCauliflower mosaic virus (CaMV) 35S promoterAvailable SinceJan. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pL
Plasmid#196286Purpose35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)DepositorInsertFull length TSWV L antigenome encoding codon-optimized L (RdRp)
UseCRISPRExpressionPlantPromoterduplicated cauliflower mosaic virus 35S promoterAvailable SinceApril 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-Pontin
Plasmid#51635PurposeMammalian expression of 3xFLAG tagged pontin, N-terminal.DepositorAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pSC-humanADAR1
Plasmid#198235PurposeExpresses human ADAR1 in yeastDepositorAvailable SinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES
Plasmid#69909PurposeExpresses a GFP circular RNA containing the sense EMCV IRES in mammalian cellsDepositorAvailable SinceOct. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDEST40-MFRN2
Plasmid#50546PurposeExpression of MFRN2 gene in mammalian cellsDepositorAvailable SinceJan. 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEM047 [T7-assisted CROPseq sgRNA expression vector]
Plasmid#224899PurposeLentiviral CROP-seq vector with lineage barcode cassette and T7 promoter for ID amplification from cDNADepositorInsertseGFP
LacZ fragment (removed by digestion with BstXI/BlpI for sgRNA cloning)
UseCRISPR and LentiviralExpressionMammalianPromoterEF-1aAvailable SinceAug. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA1&2
Plasmid#86087PurposeSimultaneous expression of cDNAs of complete genomic RNA1 and RNA 2 of PNRSV (Pch12 isolate) in transfected cellsDepositorInsertRNA1, RNA2
ExpressionPlantPromoter35SAvailable SinceFeb. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA3
Plasmid#86088PurposeExpression of cDNA of full-length genomic RNA3 of PNRSV (Pch12 isolate) in transfected cellsDepositorInsertRNA3
ExpressionPlantPromoter35SAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBHM2329
Plasmid#171686PurposepRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCRDepositorInsertPCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastPromoterC. neoformans U6Available SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRSetB-KanR-LiLac
Plasmid#184569PurposeFluorescent reporter for lactate (with lifetime and intensity response); for bacterial or in vitro expressionDepositorInsertLiLac fluorescent sensor of lactate
TagsHis7ExpressionBacterialPromoterT7Available SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xFLAG-TCAB1
Plasmid#51630PurposeMammalian expression of 3xFLAG tagged TCAB1, N-terminal.DepositorAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) HIPK3 Sense
Plasmid#60634PurposeExpresses the human HIPK3 exon 2 circular RNADepositorAvailable SinceJan. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRII_TOPO_hNEAT1
Plasmid#61518Purposehuman NEAT1_v1 short isoform 3.7kb insert in general cloning vectorDepositorAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBK1340-LV-DUAL-dGFP-NanoLuc-Puro
Plasmid#223160PurposeExpression of destabilized GFP (dGFP) and NanoLuc for shortened in vivo half-lifeDepositorInsertdGFP-P2A-NanoLuc
UseLentiviralExpressionMammalianPromoterCMVAvailable SinceAug. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
miRFP670-TRF1
Plasmid#227388PurposeLabel telomere, no adhesionDepositorInsertTelomeric miRFP (TERF1 Human)
UseLentiviralAvailable SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBS FGF3 isoform 1
Plasmid#19230DepositorInsertFibroblast growth factor receptor 3 isoform 1 (FGFR3 Chicken)
ExpressionBacterialAvailable SinceSept. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
pGTMV
Plasmid#118755PurposeBinary plasmid with a tobacco mosaic virus infectious clone; Agrobacterium-mediated transformation required for expression in plants.DepositorInsertTobacco mosaic virus
ExpressionPlantPromoterCauliflower mosaic virus 35SAvailable SinceNov. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDEST47-SAR1-GFP
Plasmid#67409PurposeMammalian expression of hSar1 with C-term GFP tagDepositorAvailable SinceOct. 5, 2015AvailabilityAcademic Institutions and Nonprofits only -
AAV-FLIM-AKAR
Plasmid#63058PurposeExpresses the Fluorescence Lifetime Imaging Microscopy (FLIM)-compatible PKA activity sensor FLIM-AKAR in an AAV backboneDepositorInsertFLIM-AKAR
UseAAVExpressionMammalianPromoterEF-1αAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNCS mStrawberry
Plasmid#91768PurposeBacterial expression of a fluorescent protein, monomeric Strawberry (mStrawberry)DepositorInsertmonomeric Strawberry
TagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialPromoterT7Available SinceJune 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pVAX1/mTyr-Cre
Plasmid#99249PurposeExpresses Cre recombinase under tyrosinase promoterDepositorInsertsCre recombinase
mTyr enh/prom
TagsMycExpressionMammalianAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-3xHA-Reptin
Plasmid#51636PurposeMamamlian expression of 3xHA tagged reptin, N-terminal.DepositorAvailable SinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) Laccase2 MCS Exon Vector
Plasmid#69893PurposeExpression plasmid for expressing circular RNAs of a desired sequence in mammalian cellsDepositorAvailable SinceMarch 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
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Cytosolic Syk Biosensor
Plasmid#125729PurposeFRET biosensor for studying SYK activities in cellsDepositorAvailable SinceNov. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB/pHSP-tTA-TRE-tTA-pEF1α-PuroR
Plasmid#206213PurposePiggyBac-based transposon vector plasmid which encodes an expression unit of tetracycline-responsive transactivator under control of HSP promoter with transcriptional amplificationDepositorInserttetracycline-responsive transactivator
ExpressionMammalianAvailable SinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGal4x5-E4T-G210
Plasmid#171077PurposeThe resulting plasmid was used to generate a Gal4x5-E4-Gless 210bp construct for ampilifying the immobilized template for assays. Originally came from Michael Carey, was submitted with his permissionDepositorInsertGal4-E4-Gless-cassette
ExpressionBacterialPromoterT7 promoterAvailable SinceJuly 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPEPY-PF6-lacI
Plasmid#85589PurposeWork as template for amplification of Streptococcus pneumoniae adapted lacI gene, which is flanked by up- and downstream sequence for integration in prsA locus in S. pneumoniaeDepositorInsertlacI
ExpressionBacterialPromoterPF6Available SinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only