We narrowed to 6,963 results for: RAP

1. DCT.VV.Orf188

   Plasmid

   #12876

   Purpose

   This plasmid was designed for screening purposes and may contain discrepancies relative to the current canonical GenBank version of the gene.

   Depositor

   Jack Bennink, Jon Yewdell

   Article

   Tscharke et al J Exp Med. 2005 January 3; 201(1): 95–10

   Insert

   VV.Orf188
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-Cerulean-Epac2(R53A)-Venus

   Plasmid

   #138197

   Purpose

   FRET-based Epac2 activation sensor with R53A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R53A)-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-Cerulean-Epac2(R447)-cpV[E172]

   Plasmid

   #138198

   Purpose

   FRET-based Epac2 activation sensor with R447A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R447A)-cpVenus[E172] (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and cpV[E172]

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-Cerulean-Epac2(R466A)-Venus

   Plasmid

   #138199

   Purpose

   FRET-based Epac2 activation sensor with R466A mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(R466A)-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains arginine-to-alanine substitution at resi…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3.1-Cerulean-Epac2(L426W)-Venus

   Plasmid

   #138200

   Purpose

   FRET-based Epac2 activation sensor with L426W mutation in Epac2 coding sequence.

   Depositor

   Jin Zhang

   Article

   Herbst et al Chem Biol. 2011 Feb 25;18(2):243-51. doi

   Insert

   Cerulean-Epac2(L426W)-Venus (Rapgef4 Synthetic, Mouse)
   

   Use

   Tags

   Cerulean and Venus

   Expression

   Mammalian

   Mutation

   Contains leucine-to-tryptophan substitution at re…

   Promoter

   CMV

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV/D374Y-hPCSK9

   Plasmid

   #58379

   Purpose

   Expresses GoF mutant human PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Insert

   proprotein convertase subtilisin/kexin type 9 (PCSK9 Human)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D374Y

   Promoter

   HCRApoE/hAAT

   Available since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Lyn-ICUE3

   Plasmid

   #61623

   Purpose

   Plasma membrane-targeted FRET biosensor for monitoring cAMP

   Depositor

   Jin Zhang

   Article

   DiPilato et al Mol Biosyst. 2009 Aug;5(8):832-7. doi: 1

   Insert

   Lyn-ICUE3 (RAPGEF3 Human)
   

   Use

   Tags

   ECFP, N-terminal targeting sequence from Lyn kina…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAAV/D377Y-mPCSK9

   Plasmid

   #58376

   Purpose

   Expresses GoF mutant murine PCSK9 to be used for rAAV8-mediated gene transfer, hypercholesterolemia and atherosclerosis

   Depositor

   Jacob Bentzon

   Article

   Bjorklund et al Circ Res. 2014 May 23;114(11):1684-9. do

   Insert

   proprotein convertase subtilisin/kexin type 9 (Pcsk9 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   D377Y

   Promoter

   HCRApoE/hAAT

   Available since

   Aug. 26, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GFP-AHPH-DM

   Plasmid

   #71368

   Purpose

   Location sensor for RhoA-GTP

   Depositor

   Alpha Yap

   Article

   Priya et al Nat Cell Biol. 2015 Oct;17(10):1282-93.

   Insert

   C terminus of Anillin (ANLN Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   A740D and E758K

   Promoter

   CMV

   Available since

   Nov. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-eNme2-C-BE4max

    Plasmid

    #183679

    Purpose

    Expresses eNme2-C-BE4 in mammalian cells

    Depositor

    David Liu

    Article

    Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

    Insert

    rAPOBEC1-neNme2-C-2xUGI
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

    Promoter

    Available since

    Oct. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTK Cas9-2A-Citrine

    Plasmid

    #92393

    Purpose

    Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Development. 2018 Feb 23;145(4). pii: de

    Insert

    Cas9 2A Citrine
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    thymidine kinase promoter

    Available since

    Aug. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMX-SHP1C/S-ires-mCD8

    Plasmid

    #183719

    Purpose

    Expresses the myc-tagged SHP-1 C453S mutant (SHP-1C/S) (substrate-trapping mutant) and mouse CD8.

    Depositor

    Takeshi Tsubata

    Article

    Alborzian Deh Sheikh et al J Immunol. 2021 Jun 1;206(11):2544-2551.

    Insert

    SHP-1
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mutation

    C453S

    Promoter

    Available since

    June 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28C-mCherry-MED1LCD

    Plasmid

    #204404

    Purpose

    E. coli expression of MED1 low-complexity domain (947-1581) with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    MED1(947-1581) (MED1 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1 HA-FAM208A

    Plasmid

    #115845

    Purpose

    Encodes codon optimized HA tagged FAM208A

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    HA tagged FAM208A (C3orf63 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pAPM-D4 miR30-FAM208A ts2

    Plasmid

    #115872

    Purpose

    FAM208a knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    FAM208a shRNA (C3orf63 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAPM-D4 miR30-FAM208A ts3

    Plasmid

    #115873

    Purpose

    FAM208a knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    FAM208a shRNA (C3orf63 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUAST-attB-3xHA-V5-RpL10Ab

    Plasmid

    #125223

    Purpose

    3xHA-V5 tagged RpL10Ab for expression from UAS promoter to TRAP translatome from specific cell types defined by GAL4 expression

    Depositor

    Herman Dierick

    Article

    Thomas et al PLoS One. 2012;7(7):e40276. doi: 10.1371

    Insert

    RpL10Ab (RpL10Ab Fly)
    

    Use

    Tags

    3x HA, V5

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAVS1-PdTK-CAG-mCh-[uBglII]

    Plasmid

    #107280

    Purpose

    puro-deltaTK gene-trap selection cassette (AAVS1 donor vector backbone) with constitutive mCherry

    Depositor

    Knut Woltjen

    Article

    Kim et al Nat Commun. 2018 Mar 5;9(1):939. doi: 10

    Insert

    AAVS1 puro-deltaTK; CAG-mCherry (PPP1R12C )
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAPM-D4 miR30-FAM208A ts1

    Plasmid

    #115871

    Purpose

    FAM208a knockdown

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

    Insert

    FAM208a shRNA (C3orf63 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA5-eYFP-FKBP-MAD1-WT

    Plasmid

    #114037

    Purpose

    Chemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)

    Depositor

    Geert Kops

    Article

    Kuijt et al Chromosoma. 2014 Oct;123(5):471-80. doi:

    Insert

    Human MAD1 (MAD1L1 Human)
    

    Use

    Tags

    eYFP-FKBP12

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CMV

    Available since

    Oct. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pAd5-Blue

    Plasmid

    #174431

    Purpose

    pAd5-Blue provides a platform for the rapid construction of recombinant and replication defective Adenovirus. This vector contains unique restriction enzyme sites to allow cloning of a gene.

    Depositor

    Teresa De Los Santos

    Article

    Fernandez-Sainz et al Virology. 2017 Feb;502:123-132. doi: 10.

    Insert

    β-galactosidase α gene fragment
    

    Use

    Adenoviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. EGFP-C1-FKBP-CYB5tail

    Plasmid

    #202686

    Purpose

    rapamycin-induced recruitment of proteins to ER

    Depositor

    Gerry Hammond

    Article

    Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

    Insert

    EGFP:FKBP:[GGSA]4GG:CYB5A(100-134) (CYB5A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX459-HypaCas9-ANLN_sgRNA

    Plasmid

    #183874

    Purpose

    pX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    ANLN sgRNA spacer (ANLN Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-ACBEmax

    Plasmid

    #139106

    Purpose

    Mammalian ACBEmax expression vector (pSI-632)

    Depositor

    Nozomu Yachie

    Article

    Sakata et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

    Insert

    Bi_partite_NLS-TadA*-XTEN_linker-TadA-XTEN_linker-SpCas9(D10A)-SH3_linker-rAPOBEC1-2xUGI-SV40NLS
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJW1846

    Plasmid

    #154326

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::GFP^SEC^AID*::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    F+E mutations in sgRNA

    Promoter

    Available since

    Aug. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. FKBP-Stx3-mCitrine

    Plasmid

    #92428

    Purpose

    Expression of syntaxin-3 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLV-mCherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176138

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCYC1pr-GNSI (LEU)

    Plasmid

    #111450

    Purpose

    GNSI is a synthetic, genetically-encoded reporter that allows rapid plate-based assessment of AP-3 functional deficiency, using either colorimetric or growth phenotype readouts.

    Depositor

    Alexey Merz

    Article

    Plemel et al Traffic. 2020 Nov 23. doi: 10.1111/tra.1

    Inserts

    CYC1 Promoter (CYC1 Budding Yeast)
    Nyv1 Cytosolic Domain (aa 6-230)
    Snc1 Transmembrane Domain (TMD)
    SUC2 CDS
    SUC2 terminator
    

    Use

    Tags

    mGFP5

    Expression

    Yeast

    Mutation

    M109I (Naturally occurring SNP) and The first 21 …

    Promoter

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. FKBP-Stx4-mCitrine

    Plasmid

    #92427

    Purpose

    Expression of syntaxin-4 N-terminally conjugated to FKBP and C-terminally conjugated to mCitrine.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx4 (Stx4 Rat)
    

    Use

    Tags

    FKBP (FK506 binding protein 12); allows for heter…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3-FKBP-ICUE3-NLS

    Plasmid

    #138255

    Purpose

    FRET-based cAMP indicator fused to FKBP (rapamycin-binding domain); nuclear targeted. Used in conjuction with pcDNA3.1(+)-AKAP95-FRB to monitor cAMP dynamics within AKAP95 nanodomain.

    Depositor

    Jin Zhang

    Article

    Clister et al Cell Chem Biol. 2019 Jun 20;26(6):885-89

    Insert

    FKBP-ICUE3-NLS
    

    Use

    Tags

    FKBP, Nuclear localization signal (NLS), cpVenus[…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 31, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. sgRNA1_IL1RN

    Plasmid

    #64140

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. sgRNA3_IL1RN

    Plasmid

    #64151

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA3_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pLenti-X1-Neo-GFP-ATL2-deltaTM

    Plasmid

    #109021

    Purpose

    stable lentiviral expression of cDNA

    Depositor

    Jacob Corn

    Article

    Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

    Insert

    ATL2-deltaTM (ATL2 Human)
    

    Use

    Lentiviral

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    truncated form without C-terminal ER transmembran…

    Promoter

    EIF-1a promoter

    Available since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. SRAcode_in_pUC57_v3

    Plasmid

    #49822

    Purpose

    for transcribing SRA nucl 419-1356 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Jan. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. sgRNA2_IL1RN

    Plasmid

    #64142

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA2_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. sgRNA4_IL1RN

    Plasmid

    #64152

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA4_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pADH1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. SRAcode_in_pUC57_v3(767-1356)

    Plasmid

    #49826

    Purpose

    for transcribing SRA nucl 767-1356 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pEntr1a-mcherry-FKBP-TIAM1(DHPH)

    Plasmid

    #176135

    Purpose

    Heterodimerization with FRB (rapamycin), Rac1 activation

    Depositor

    Dorus Gadella

    Article

    Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

    Insert

    TIAM1 (TIAM1 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    See Depositor Comments Below

    Promoter

    Available since

    Oct. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCS2 Notch1 LNG-6MT

    Plasmid

    #41738

    Depositor

    Jeffrey Nye, Raphael Kopan

    Article

    Kopan et al Proc Natl Acad Sci U S A. 1996 Feb 20;93

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available since

    Jan. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

41. pJW1886

    Plasmid

    #154333

    Purpose

    Multi-cassette for SapTrap

    Depositor

    Jordan Ward

    Article

    Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

    Insert

    30aa linker::mScarlet-I^SEC (Lox511I)^TEV::degron::3xFLAG
    

    Use

    CRISPR and Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    Promoter

    Available since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pcDNA3.1(-)-HF2-BE2

    Plasmid

    #120396

    Purpose

    Expresses HF2-BE2 in mammalian cells

    Depositor

    Junjiu Huang

    Article

    Liang et al Protein Cell. 2017 Aug;8(8):601-611. doi

    Insert

    HF2-BE2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mammalian codon-optimized Cas9-HF

    Promoter

    CMV

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pEF-HA-TSAd-d239-74

    Plasmid

    #84560

    Purpose

    mammalian expression of TSAd-d239-74

    Depositor

    Anne Spurkland

    Article

    Berge et al PLoS One. 2010 Mar 18;5(3):e9761. doi: 1

    Insert

    TSAd (SH2D2A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    deletion of aa 239-274

    Promoter

    EF1a

    Available since

    Nov. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

44. pCS2 Notch1 LNG (CC→SS)-6MT

    Plasmid

    #41739

    Depositor

    Raphael Kopan

    Article

    Mumm et al Mol Cell. 2000 Feb;5(2):197-206.

    Insert

    Notch-1 Lin-Notch-glp (LNG) repeat-containing construct, CC->SS (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    Contains murine Notch-1 leader peptide (aa 1-23) …

    Promoter

    Available since

    Dec. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

45. SRAcode_in_pUC57_v3(1000-1356)

    Plasmid

    #49827

    Purpose

    for transcribing SRA nucl 1000-1356 of NM_001035235 with T7

    Depositor

    Thomas Cech

    Article

    McKay et al J Mol Biol. 2014 Apr 17;426(8):1766-85.

    Insert

    steroid receptor RNA activator RNA coding (SRA1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    KpnI site at 5' start of RNA coding seq

    Promoter

    T7

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pHR TRE_IL1Ra-PGK_BFP

    Plasmid

    #223549

    Purpose

    Inducible IL-1Ra; constitutive BFP

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Inserts

    IL-1Ra (IL1RN Human)
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. MZB150

    Plasmid

    #217840

    Purpose

    Bacterial expression of 10xHis-SUMO (Smt3) fusion to Sec7 domain (52-252) of Cytohesin-2 (CYTH2/ARNO) for rapid nucleotide exchange and activation of Arf GTPases

    Depositor

    Richard Baker

    Article

    Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

    Insert

    ARNO (CYTH2 Human)
    

    Use

    Tags

    10x His and SUMO (SMT3)

    Expression

    Bacterial

    Mutation

    Deleted residues 1-51 and 253-400

    Promoter

    Available since

    May 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. L2096-GPA-BRET-Biosensor-Retrovirus-TD146

    Plasmid

    #222878

    Purpose

    Retroviral vector encoding biosensor chains to detect rapamycin (FRB/FKBP domains) and respond with BRET (split NanoLuciferase reconstitution [11S/114 fragment]). WT Glycophorin A (GPA) scaffold.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-NanoLuc114-CyOFP1-T2A-FKBP-GPA-NanoLuc11S
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    MSCV LTR

    Available since

    Sept. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. L2117-FKBP-GPA-114-RH-CyOFP1

    Plasmid

    #222884

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FKBP-GPA-20GS-NanoLuc114-RH-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. L2116-FRB-GPA-114-RH-CyOFP1

    Plasmid

    #222883

    Purpose

    Biosensor chain detecting rapamycin and responding with BRET. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114, RH linker, CyOFP1 protein.

    Depositor

    Joshua Leonard

    Article

    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

    Insert

    FRB-GPA-20GS-NanoLuc114-RH-CyOFP1
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge