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pmax pona 12xTRICK 24xMS2SL

Plasmid

#64542

Purpose

TRICK translation biosensor with 12x copies of modified PP7 stem loops and 24x MS2 stem loops.

Depositor

Jeffrey Chao

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

12x PP7 stem-loops

Tags

24xMS2 stem loops, HA, NLS, and dNP799

Expression

Mammalian

Promoter

ponA

Available Since

May 7, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA-D3cpv

Plasmid

#36323

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

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pT3-EF1a-Bmi1

Plasmid

#31783

Depositor

Xin Chen

Article

Xu et al Mol Cancer Res. 2009 Dec;7(12):1937-45.

Insert

Bmi1 (BMI1 Human)

Tags

HA

Expression

Mammalian

Promoter

EF1alpha

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pRRL SFFV d20GFP.T2A.mTagBFP Donor

Plasmid

#31485

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2011 Jul 10;8(8):671-6. doi

Insert

SFFV d20BFP T2A BFP

Use

Lentiviral

Mutation

GFP has 20 amino acids deleted from the C terminu…

Available Since

Aug. 18, 2011

Availability

Academic Institutions and Nonprofits only

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pRsetB-pHRed

Plasmid

#31472

Depositor

Gary Yellen

Article

Tantama et al J Am Chem Soc. 2011 Jul 6. 133(26):10034

Insert

pHRed

Tags

7xHis

Expression

Bacterial

Available Since

July 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2 HRS-RFP

Plasmid

#29685

Depositor

Edward De Robertis

Article

Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

Insert

hepatocyte growth factor-regulated tyrosine kinase substrate (HGS Human)

Tags

RFP

Expression

Mammalian

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-JMJD2B

Plasmid

#24181

Purpose

Mammalian expression of human JMJD2B with HA tag

Depositor

Kristian Helin

Article

Cloos et al Nature. 2006 Jul 20. 442(7100):307-11.

Insert

JMJD2B (KDM4B Human)

Tags

HA

Expression

Mammalian

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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EdTP

Plasmid

#24311

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

EF1alpha-dnhTcf4 // SV40-PuroR (TCF7L2 Human)

Use

Lentiviral

Tags

FLAG

Mutation

31 first amino acids of hTcf4 have been deleted.

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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Flag-HA-OTUB1

Plasmid

#22551

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

OTUB1 (OTUB1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 16, 2009

Availability

Academic Institutions and Nonprofits only

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Luc.Cre empty vector

Plasmid

#20905

Depositor

Tyler Jacks

Type

Empty backbone

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Available Since

April 22, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA2-ROC1

Plasmid

#19897

Depositor

Yue Xiong

Article

Ohta et al Mol Cell. 1999 Apr . 3(4):535-41.

Insert

RING finger protein 1 (ROC1) (RBX1 Human)

Tags

HA

Expression

Mammalian

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only

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GFP-TXNIP

Plasmid

#18758

Depositor

Clark Distelhorst

Article

Wang et al Oncogene. 2006 Mar 23. 25(13):1903-13.

Insert

thioredoxin-interacting protein (Txnip Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Aug. 4, 2008

Availability

Academic Institutions and Nonprofits only

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pCBh-NLS_hfCas13d(RfxCas13d_N2V8)_HA_NLS-pA-U6-DR-BpiI-BpiI-pSV40-EGFP-pA-pSV40-mCherry-pA

Plasmid

#190034

Purpose

vector for encoding a human codon-optimized High-fidelity RfxCas13d (hfCas13d) driven by CBh promoter, guide RNAs compatible with RfxCas13d driven by hU6, EGFP and mCherry driven by SV40 promoters

Depositor

Huawei Tong

Article

Tong et al Nat Biotechnol. 2022 Aug 11. pii: 10.103

Insert

humanized hfCas13d

Expression

Mammalian

Mutation

A134V,A140V,A141V,A143V

Promoter

CBh, SV40, hU6

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-CMV-N-EGFP

Plasmid

#122842

Purpose

N-terminal EGFP tag. Gateway destination vector for mammalian expression.

Depositor

Robin Ketteler

Article

Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

Type

Empty backbone

Use

Gateway Cloning

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCS2+mNeonGreen-C Cloning Vector

Plasmid

#128144

Purpose

To express a protein of interest fused to the C-terminus of mNeonGreen

Depositor

Ken-Ichi Takemaru

Type

Empty backbone

Tags

mNeonGreen fusion

Expression

Mammalian

Available Since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti6.2_mTagBFP2

Plasmid

#113725

Purpose

Expresses the fluorescent protein mTagBFP2 in a lentiviral backbone

Depositor

Vanessa LaPointe

Article

Wieland et al Front Bioeng Biotechnol. 2021 Sep 9;9:72

Insert

mTagBFP2

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pX330A-nBRD4/PITCh

Plasmid

#91794

Purpose

PITCh sgRNA, N-terminal BRD4 sgRNA, and Cas9 expressing plasmid for use with the dTAG knock-in system and BRD4

Depositor

James Bradner, Behnam Nabet

Article

Nabet et al Nat Chem Biol. 2018 May;14(5):431-441. d

Insert

SpCas9

Use

CRISPR

Expression

Mammalian

Promoter

chicken β-actin promoter

Available Since

March 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.TBG.PI.eGFP.WPRE.bGH

Plasmid

#105535

Purpose

AAV expression of EGFP from TBG promoter

Depositor

James M. Wilson

Has Service

AAV8

Insert

EGFP

Use

AAV

Expression

Mammalian

Promoter

TBG

Available Since

Feb. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pET259-pUC57 24xMS2V6

Plasmid

#104391

Purpose

MS2V6 cloning

Depositor

Robert Singer, Evelina Tutucci

Article

Tutucci et al Nat Methods. 2017 Nov 13. doi: 10.1038/n

Insert

24xMS2V6

Expression

Bacterial

Mutation

All Loops are U-variants, interspaced by 50 nucle…

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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p11d-tRPA(123)

Plasmid

#102613

Purpose

Expression of human RPA in E. coli

Depositor

Marc Wold

Article

Henricksen et al J Biol Chem. 1994 Apr 15;269(15):11121-3

Inserts

RPA1 (RPA1 Human)
RPA2 (RPA2 Human)
RPA3 (RPA3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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