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pDEST26-USP47

Plasmid

#64326

Purpose

Mammalian expression and purification of USP47.

Depositor

Grigory Dianov

Article

Parsons et al Mol Cell. 2011 Mar 4;41(5):609-15. doi:

Insert

USP47 (USP47 Human)

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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pRevTRE MKL1

Plasmid

#19846

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 (MRTFA Human)

Use

Retroviral

Tags

3xFLAG

Available Since

Dec. 16, 2008

Availability

Academic Institutions and Nonprofits only
pQE60 R124A wgMDH

Plasmid

#204257

Purpose

Bacterial expression of R124A wgMDH with IPTG induction - Active site mutant

Depositor

Malate dehydrogenase CUREs Community (MCC)

Article

Cox et al FEBS J. 2005 Feb;272(3):643-54. doi: 10.

Insert

MDHG_CITLA

Tags

6X His

Expression

Bacterial

Mutation

R124A

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSLQ2814 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SpdCas9

Plasmid

#84247

Purpose

Expresses direct fusion VPR-Sp dCas9

Depositor

Stanley Qi

Article

Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

Inserts

VPR-tagBFP-Sp dCas9
Puro

Use

CRISPR and Synthetic Biology; Piggybac

Tags

2xNLS (SV40), HA tag, VPR, and tagBFP

Expression

Mammalian

Promoter

CAG and PGK

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV-5U-Venus-PSD-95-3U

Plasmid

#102949

Purpose

Expresses Venus-PSD-95 in mammalian cells

Depositor

Gary Bassell

Article

Ifrim et al J Neurosci. 2015 May 6;35(18):7116-30. d

Insert

PSD-95 (Dlg4 Mouse)

Tags

Venus Fluorescent protein

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCDH_Str-KDEL_ManII-SBP-mCherry

Plasmid

#65259

Purpose

synchronize trafficking of ManII from the ER (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

Streptavidin-KDEL and truncated Mannosidase II

Use

Lentiviral

Tags

Streptavidin Binding Protein (SBP) and mCherry

Mutation

contains only amino acids 1 to 116 of ManII

Promoter

CMV

Available Since

Aug. 3, 2015

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1750-2549 aa)

Plasmid

#21747

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1750-2549) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1750-2549

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only
pHsp83-NLS-HA-2xPCP-2xGFP

Plasmid

#71243

Purpose

Used to label reporter mRNAs. Drosophila expression of the PCP protein fused to EGFP.

Depositor

Anne Ephrussi

Article

Halstead et al Science. 2015 Mar 20;347(6228):1367-671.

Insert

2xPCP-2xGFP

Use

P element-mediated germline transformation

Tags

EGFP

Expression

Insect

Promoter

Hsp83

Available Since

Dec. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA6-IgK-hFRa(23-234)C11S-th-Fc

Plasmid

#177841

Purpose

Mammalian Expression of Folate Receptor

Depositor

Karsten Melcher

Article

Chen et al Nature. 2013 Aug 22;500(7463):486-9. doi

Insert

Folate receptor alpha (FOLR1 Human)

Expression

Mammalian

Mutation

all 11 C mutated to S

Available Since

Dec. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

Plasmid

#166611

Purpose

Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

EGFP-2A-Tetanus Toxin

Use

AAV

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-ChR2-TdTomato (Cre-OFF)

Plasmid

#166610

Purpose

Encodes Cre-inactivated ChR2-TdTomato under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

ChR2-TdTomato

Use

AAV

Mutation

H134R

Promoter

tetracycline response element

Available Since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2/Capsid

Plasmid

#120816

Purpose

Ectopic expression of ZIKV protein

Depositor

Jan Rehwinkel

Article

Hertzog et al Eur J Immunol. 2018 Mar 23. doi: 10.1002

Insert

Capsid ZIKV

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pUCHR-osTIR1-IRES-GFP

Plasmid

#110661

Purpose

Lentiviral vector for stable expression of osTIR1 and selection with GFP

Depositor

Dmitriy Mazurov

Article

Zotova et al Sci Rep. 2019 Feb 28;9(1):3132. doi: 10.

Insert

osTIR1

Use

Lentiviral

Promoter

CMV

Available Since

March 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX303-GFP

Plasmid

#162032

Purpose

Tagged vector control

Depositor

David Bryant

Article

Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Available Since

Dec. 4, 2020

Availability

Academic Institutions and Nonprofits only

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myc-mTOR (1271-2008 aa)

Plasmid

#21746

Depositor

David Sabatini

Article

Kim et al Mol Cell 2003 Apr;11(4):895-904.

Insert

mTOR (1271-2008) (Mtor Rat)

Tags

myc

Expression

Mammalian

Mutation

amino acids 1271-2008

Available Since

Aug. 18, 2009

Availability

Academic Institutions and Nonprofits only
pClneoMyc human NDR1

Plasmid

#37023

Depositor

Yutaka Hata

Article

Hirabayashi et al Oncogene. 2008 Jul 17;27(31):4281-92. Ep

Insert

NDR1 (STK38 Human)

Tags

myc

Expression

Mammalian

Promoter

CMV

Available Since

May 23, 2012

Availability

Academic Institutions and Nonprofits only

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pMRXIB-WIPI2b-mRuby3

Plasmid

#221749

Purpose

Stable expression of WIPI2b-mRuby3 in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

WIPI2b (WIPI2 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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GST-Hsp90 C(626-732)

Plasmid

#22483

Depositor

William Sessa

Article

Fontana et al Circ Res. 2002 May 3. 90(8):866-73.

Insert

Hsp90alpha C domain (HSP90AA2P Human)

Tags

GST

Expression

Bacterial

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-HA-hTet1CD-WPRE-PolyA

Plasmid

#39454

Depositor

Hongjun Song

Article

Guo et al Cell. 2011 Apr 29;145(3):423-34. Epub 20

Insert

HA-hTet1CD (TET1 Human)

Use

AAV

Tags

HA

Expression

Mammalian

Mutation

aa1418–2136

Promoter

EF1a

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
ATF4 6: mCD36.GFP

Plasmid

#21853

Depositor

David Ron

Article

Lu et al J Cell Biol. 2004 Oct 11. 167(1):27-33.

Insert

mCD36 (Cd36 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Only the first 13 AA of CD36 are fused to GFP

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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