We narrowed to 17,071 results for: Por

1. pAL189

   Plasmid

   #22278

   Purpose

   Expresses Intersectin DH-PH domains fused to mYFP-PIF6, for optogenetic recruitment

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   IntersectinDHPH-20aaLinker-mYFP-PIF6APB (PIL2 Mustard Weed)
   

   Use

   Synthetic Biology

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Oct. 21, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUC19-Split-Broccoli-Toehold-Switch

   Plasmid

   #87316

   Purpose

   Expresses the Split-Broccoli system and a Trigger/Toehold switch for mCherry.

   Depositor

   Donald Burke

   Article

   Alam et al ACS Synth Biol. 2017 May 26. doi: 10.102

   Insert

   Split-Broccoli system and RNA Trigger (TS2_AT01) Toehold (TS2_KS01) switch for mCherry
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   P70a

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pSV(X) C/EBPalpha

   Plasmid

   #13392

   Depositor

   Richard Schwartz

   Article

   Hu et al J Immunol. 1998 Mar 1. 160(5):2334-42.

   Insert

   C/EBPalpha (Cebpa Rat)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pXCX.CMV.CREB.WPRE

   Plasmid

   #38050

   Depositor

   James Uney

   Article

   Glover et al Neuroreport. 2004 May 19;15(7):1171-5.

   Insert

   CREB (Creb1 Rat)
   

   Use

   Adenoviral

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 15, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. myc-Orai1 E106A pHAGE

   Plasmid

   #22752

   Depositor

   Anjana Rao

   Article

   Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

   Insert

   Orai1 E106A (ORAI1 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   E106A

   Available Since

   Jan. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

6. pTW102

   Plasmid

   #115945

   Purpose

   ORF insert for replicon MoClo

   Depositor

   Ron Weiss

   Article

   Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

   Insert

   Lvl0 DDd-L7Ae
   

   Use

   Synthetic Biology

   Available Since

   May 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pPotef-LN22*-3xGNLS-cbx

   Plasmid

   #86780

   Purpose

   Expresses lambda N protein fused to triple Gfp coupled to an NLS sequence under the control of the constitutively active Potef promotor.

   Depositor

   Michael Feldbrügge

   Article

   Pohlmann et al Elife. 2015 May 18;4. doi: 10.7554/eLife

   Insert

   LN*
   

   Use

   Ustilago maydis expression

   Tags

   Gfp

   Expression

   Bacterial

   Promoter

   Potef

   Available Since

   Sept. 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCVL.SFFV.Kozak.HA.NLS.Y2Ani.IRES.BFP

   Plasmid

   #45578

   Purpose

   Expresses Y2 variant of I-Ani I nuclease in mammalian cells with BFP tracker

   Depositor

   Andrew Scharenberg

   Article

   Kuhar et al Nucleic Acids Res. 2013 Oct 10.

   Insert

   coY2 Ani
   

   Use

   Lentiviral

   Tags

   HA, IRES BFP, and NLS

   Expression

   Mammalian

   Mutation

   co indicates codon optimization for mammalian exp…

   Promoter

   SFFV

   Available Since

   Nov. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. PB-Pitx2

   Plasmid

   #60668

   Purpose

   PiggyBac expression vector encoding murine Pitx2 downstream of a doxycycline inducible promoter

   Depositor

   James Thomson

   Article

   Vereide et al Stem Cell Reports. 2014 Dec 9;3(6):1043-

   Insert

   Pitx2 (Pitx2 Mouse)
   

   Use

   Piggybac

   Expression

   Mammalian

   Available Since

   Sept. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHA-ΔN332-Kin1C

    Plasmid

    #31608

    Depositor

    Anthony Brown

    Article

    Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

    Insert

    ΔN332 kinesin-1C (Kif5c Mouse)
    

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

11. A3D-Cas9n-UGI

    Plasmid

    #119137

    Purpose

    Base editor made from APOBEC3D

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3D-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pLenti-hU6-mU6-Cre2GO

    Plasmid

    #136907

    Purpose

    Lentivirus for base editing activatable Cre recombinase expression in mammalian cells. All in one vector with sgCre2GO.

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    BlasCyGO
    

    Use

    Lentiviral

    Mutation

    ATG>ACG

    Promoter

    SFFV

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pStr-KDEL_EPV-SBP-DsRed2-ER-5

    Plasmid

    #170676

    Purpose

    Expresses ER-targeted streptavidin-KDEL (hook) and SBP-DsRed2 targeted to the ER lumen with an N-terminal EPV tripeptide (cargo) for RUSH.

    Depositor

    Ke Xu

    Article

    Yan et al Dev Cell. 2022 Feb 28;57(4):512-525.e8.

    Inserts

    EPV-SBP-DsRed2-ER-5
    streptavidin-KDEL
    

    Expression

    Mammalian

    Promoter

    IRES and T7

    Available Since

    Jan. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPPC017.109

    Plasmid

    #171145

    Purpose

    Expression of J1-BBa_J23117-mRFP and J109 scRNA on pBBR1-KmR plasmid

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al Metab Eng. 2021 Apr 27. pii: S1096-7176(

    Inserts

    J109 scRNA
    J1-BBa_J23117-mRFP
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    BBa_J23119 and J1-BBa_J23117

    Available Since

    Oct. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pTrc99-Rat FABP2

    Plasmid

    #13578

    Depositor

    Martin Scanlon

    Article

    Velkov et al J Biol Chem. 2005 May 6. 280(18):17769-7

    Insert

    intestinal fatty acid binding protein (Fabp2 Rat)
    

    Expression

    Bacterial

    Available Since

    March 15, 2007

    Availability

    Academic Institutions and Nonprofits only

16. pB194

    Plasmid

    #61154

    Purpose

    Expression of KIN28 under GAL1 promoter in yeast

    Depositor

    Charles Brenner

    Article

    Bieganowski et al J Biol Chem. 2002 Mar 29;277(13):10852-6

    Insert

    TFIIH complex serine/threonine-protein kinase subunit KIN28 (KIN28 Budding Yeast)
    

    Expression

    Yeast

    Promoter

    Gal1

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pLenti-FNLSHiFi-P2A-Puro

    Plasmid

    #136902

    Purpose

    Lentivirus for constitutive expression of FNLSHiFi in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    FNLSHiFi
    

    Use

    Lentiviral

    Tags

    FLAG-NLS and NLS

    Mutation

    R691A

    Promoter

    EF1s

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBXPHM3-PCFT_NB

    Plasmid

    #165415

    Purpose

    Expression in E.coli of N terminally MBP tagged anti-GgPCFT nano body

    Depositor

    Simon Newstead

    Article

    Parker et al Nature. 2021 May 26. pii: 10.1038/s41586

    Insert

    GgPCFT_NB (SLC46A1 Chicken, Llama)
    

    Tags

    His10-MBP-3C protease site

    Expression

    Bacterial

    Promoter

    arabinose

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAL725

    Plasmid

    #119862

    Purpose

    precusor D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C

    Depositor

    Linda Randall

    Article

    Bariya et al J Bacteriol. 2018 Oct 1. pii: JB.00493-1

    Insert

    D-galactose/methyl-galactoside ABC transporter periplasmic binding protein, mglB, L244C, D287C (mglB Escherichia coli str. K-12)
    

    Tags

    none

    Expression

    Bacterial

    Mutation

    Leucine 244 to Cysteine, Aspartate 287 to Cysteine

    Promoter

    T5

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLD-hygro-EnVM

    Plasmid

    #24590

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VM

    Expression

    Mammalian

    Available Since

    July 7, 2010

    Availability

    Academic Institutions and Nonprofits only

21. PA-DreN

    Plasmid

    #169707

    Purpose

    The optimized DreN246-nMag

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    Dre246-nMag
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PA-DreC

    Plasmid

    #169705

    Purpose

    The optimized pMag-Dre247

    Depositor

    Dali Li

    Article

    Li et al Proc Natl Acad Sci U S A. 2020 Dec 29;11

    Insert

    pMag-Dre247
    

    Expression

    Mammalian

    Mutation

    none

    Promoter

    CAG

    Available Since

    May 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSELt_S7.A

    Plasmid

    #125835

    Purpose

    Negative selection plasmid for evolution of an amber stop codon suppression tRNA

    Depositor

    Andrew Ellington

    Article

    Maranhao et al ACS Synth Biol. 2017 Jan 20;6(1):108-119

    Insert

    ACP-2xAmb-pheS
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pr.trpL*

    Available Since

    May 15, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

24. pET20b-GpNLuc-6xHis

    Plasmid

    #135932

    Purpose

    Expresses the GpNLuc LumiFluor in bacterial cells.

    Depositor

    Antonio Amelio

    Article

    Parag-Sharma et al ACS Synth Biol. 2019 Dec 13. doi: 10.102

    Insert

    GpNLuc LumiFluor
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTK_Citrine_Nanotag_5_neg_Control

    Plasmid

    #130558

    Purpose

    Enhancer cloning nanotag reporter vector

    Depositor

    Tatjana Sauka-Spengler

    Article

    Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

    Insert

    negative oligo sequence
    

    Use

    Enhancer cloning nanotag reporter vector

    Promoter

    thymidine kinase minimal promoter

    Available Since

    Sept. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDH_ss-SBP-EGFP

    Plasmid

    #172358

    Purpose

    Reporter to monitoring a streptavidin-binding peptide trafficking

    Depositor

    Guido Kroemer

    Article

    Zhao et al Sci Rep. 2018 Oct 8;8(1):14966. doi: 10.

    Insert

    ER importing signal sequence
    

    Use

    Lentiviral

    Tags

    SBP-EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pFH2.97_PB-EPN1-MCP-T2A-eGFP-PGK-Blast

    Plasmid

    #205542

    Purpose

    Express EPN1-MCP-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN1-MCP
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid

    #69547

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pFH2.102_PB-EPN11-MCP_I-T2A-eGFP-PGK-Blast

    Plasmid

    #205547

    Purpose

    Express EPN11-MCP-I-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP-I
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFH2.103_PB-EPN11-MCP_p6-T2A-eGFP-PGK-Blast

    Plasmid

    #205548

    Purpose

    Express EPN11-MCP-p6-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11-MCP-p6
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pLD-puro-PGKnVA

    Plasmid

    #24589

    Depositor

    Jason Moffat

    Article

    Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

    Insert

    Gateway(TM) cassette
    

    Use

    Lentiviral

    Tags

    VA

    Expression

    Mammalian

    Available Since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

32. A3F-Cas9n-UGI

    Plasmid

    #119138

    Purpose

    Base editor made from APOBEC3F

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3F-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBSU6-shTim23/CMV-eGFP

    Plasmid

    #89795

    Purpose

    Expresses shRNA against TIM23 with a separate promoter for expression of GFP.

    Depositor

    Robert Friedlander

    Article

    Yano et al Nat Neurosci. 2014 Jun;17(6):822-31. doi

    Insert

    shRNA Tim23 (Timm23 Mouse)
    

    Tags

    eGFP under separate promoter

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. A3C-Cas9n-UGI

    Plasmid

    #119136

    Purpose

    Base editor made from APOBEC3C

    Depositor

    Reuben Harris

    Article

    Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

    Insert

    APOBEC3C-Cas9 nickase-UGI
    

    Use

    CRISPR

    Mutation

    None

    Promoter

    CMV

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pFH2.96_PB-EPN1-T2A-eGFP-PGK-Blast

    Plasmid

    #205541

    Purpose

    Express EPN1-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN1
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pFH2.100_PB-EPN11-T2A-eGFP-PGK-Blast

    Plasmid

    #205545

    Purpose

    Express EPN11-T2A-eGFP and blasticidin resistance marker

    Depositor

    Michael Elowitz

    Article

    Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

    Insert

    EPN11
    

    Use

    Piggybac

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pfTRAP

    Plasmid

    #50048

    Purpose

    Expresses plasmodium falciparum TRAP adhesion domains (26-299) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    plamodium falciparum TRAP (TRAP Synthetic)
    

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    C55G, N132S

    Promoter

    CMV

    Available Since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

38. pfTRAP(VWA)

    Plasmid

    #50049

    Purpose

    Expresses plasmodium falciparum TRAP VWA domain (41-240) in mammalian cells

    Depositor

    Timothy Springer

    Article

    Song et al Proc Natl Acad Sci U S A. 2012 Dec 26;10

    Insert

    pfTRAP(vwa) (TRAP Synthetic)
    

    Tags

    6Xhis tag

    Expression

    Mammalian

    Mutation

    C55G, N132S

    Promoter

    CMV

    Available Since

    Jan. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pOIMars1CyN

    Plasmid

    #75221

    Purpose

    Mammalian cell surface expression of fluorogen activating protein Mars1Cy

    Depositor

    Alan Waggoner

    Article

    Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

    Insert

    ss-HA-Mars1Cy-CD80(237-306)
    

    Tags

    HA epitope

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTK-EdnrbE2-Citrine

    Plasmid

    #127770

    Purpose

    Enhancer reporter construct containing the chicken EdnrB enhancer, E2 driving Citrine activity

    Depositor

    Tatjana Sauka-Spengler

    Article

    Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

    Insert

    ednrbE2
    

    Use

    Enhancer reporter construct

    Available Since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge