165,947 results

1. pRSETA-qmTQ2-cAMP

   Plasmid

   #225249

   Purpose

   Bacterial expression of N-terminal His-tagged qmTQ2-cAMP, a cyan fluorescent FLIM biosensor for detecting cAMP

   Depositor

   Yasushi Okada

   Article

   Zhong et al Cell Rep Methods. 2024 Nov 18;4(11):1009

   Insert

   qmTQ2-cAMP
   

   Tags

   6XHis, T7 tag, XpressTM tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pD(Ct)_FLAG3

   Plasmid

   #223896

   Purpose

   4G-cloning donor for C-terminal FLAG3 tag

   Depositor

   Stephan Gruber

   Article

   Taschner et al bioRxiv 2024.06.17.599261

   Insert

   FLAG3
   

   Use

   Golden-gate donor

   Available Since

   Sept. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPPI4-SNAP-CTLA-4-His6

   Plasmid

   #223584

   Purpose

   Protein purification of human CTLA4 with SNAP and 6xHis tags from mammalian cells

   Depositor

   Enfu Hui

   Article

   Zhao et al Immunity. 2019 Dec 17;51(6):1059-1073.e9

   Insert

   CTLA4 (CTLA4 Human)
   

   Tags

   His6 and SNAP

   Expression

   Mammalian

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

   Plasmid

   #218767

   Purpose

   encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

   Depositor

   Abhishek Chatterjee

   Article

   Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

   Insert

   EcTyrRS-pAAFRS-9
   

   Expression

   Bacterial

   Mutation

   Y37G, L71V, D182C, F183Y, L186C, D265R

   Promoter

   tacI

   Available Since

   June 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCDF-B-pC

   Plasmid

   #213495

   Purpose

   Encodes Node B of the three-node Turing circuit

   Depositor

   Mark Isalan

   Inserts

   cinI (cinI )
   lacI (lacI )
   lambda cI (lambdap50 )
   sfGFP
   

   Use

   Synthetic Biology

   Tags

   ASV tag and LVA tag

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCC1R

   Plasmid

   #213497

   Purpose

   Encodes the receptor array and inducible aiiA gene of the three-node Turing circuit

   Depositor

   Mark Isalan

   Inserts

   pcaU
   nahR
   rpaR (rpaR )
   phlF
   cinR
   vanR
   aiiA
   

   Use

   Synthetic Biology

   Available Since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET-C

   Plasmid

   #213496

   Purpose

   Encodes Node C of the three-node Turing circuit

   Depositor

   Mark Isalan

   Inserts

   lambda cI 5G6GP
   mCherry
   

   Use

   Synthetic Biology

   Tags

   ASV tag and LVA tag

   Available Since

   April 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYSD100

   Plasmid

   #212924

   Purpose

   Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3) without any signal peptide assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

   Depositor

   Paul Young

   Article

   O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

   Insert

   SUC2 (SUC2 Budding Yeast)
   

   Expression

   Yeast

   Available Since

   Feb. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p667-UBC-NRF2-E79Q-V5-miniTurbo

   Plasmid

   #207814

   Purpose

   C-terminal tagged NRF2-E79Q for miniTurbo proximity experiments

   Depositor

   Ben Major

   Article

   LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

   Insert

   NRF2-E79Q-V5-miniTurbo (NFE2L2 Human)
   

   Use

   Lentiviral

   Tags

   V5-miniTurbo

   Mutation

   E79Q

   Promoter

   UBC

   Available Since

   Dec. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. T1B/pUBQ10(mTurq->mScarlet)

    Plasmid

    #195946

    Purpose

    Bxb1 target with pUBQ10 switching from mTurq to mScarlet

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    tRBCS-mScarlet-attP_Bxb1-pUBQ10-attB_Bxb1-mTurq-tUBQ10
    

    Expression

    Plant

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. L1B1

    Plasmid

    #195944

    Purpose

    Level 1 Bxb1 cassette with constitutive promoter : pPP2AA3-Bxb1-tUBQ1

    Depositor

    Jennifer Nemhauser

    Article

    Guiziou et al Nat Commun. 2023 Apr 3;14(1):1844. doi:

    Insert

    pPP2AA3-Bxb1-tUBQ1
    

    Expression

    Plant

    Available Since

    March 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. Anti-VAChT [N425/45]

    Plasmid

    #190326

    Purpose

    Mammalian Expression Plasmid of anti-VAChT (Rat). Derived from hybridoma N425/45.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-VAChT (Rattus norvegicus) recombinant Mouse monoclonal antibody (Slc18a3 Mouse)
    

    Expression

    Mammalian

    Promoter

    Dual CMV

    Available Since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pdCas9_CL

    Plasmid

    #166245

    Purpose

    regulated dCas9 generator

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    P112-sgRNA-term-P104-RBS1-dCas9-B15
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    Ec-TTL-P112 and Ec-TTL-P104

    Available Since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHBS1392 [IBB-GFP-mCherry3E]-[BFP-P2A-TDP43 deltaNTD]

    Plasmid

    #118807

    Purpose

    To test the effect of sequence on TDP43 splicing activity

    Depositor

    Rajat Rohatgi

    Article

    Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

    Insert

    TARDBP mutant (TARDBP Human)
    

    Expression

    Mammalian

    Mutation

    Deletion of NTD in full-length TDP43

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. 4QOX

    Plasmid

    #61959

    Purpose

    Baculovirus expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-CDPK4
    

    Use

    Baculovirus expression

    Tags

    MHHHHHHSSGRENLYFQG

    Mutation

    See comments

    Promoter

    Polyhedrin

    Available Since

    May 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

16. RP185 pUAST EGFP:Or47b

    Plasmid

    #59525

    Purpose

    N-terminal fusion of GFP to Drosophila melanogaster Or47b

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or47b (Or47b Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

17. RP132 pUAST EGFP:Or43a

    Plasmid

    #59523

    Purpose

    N-terminal fusion of EGFP to Drosophila melanogaster Or43a

    Depositor

    Leslie Vosshall

    Article

    Benton et al PLoS Biol. 2006 Feb;4(2):e20. Epub 2006

    Insert

    EGFP:Or43a (Or43a Fly)
    

    Tags

    EGFP

    Expression

    Insect

    Available Since

    Sept. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

18. AiP11633 - pAAV-hsA2-AiE0140h-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1633)

    Plasmid

    #163503

    Purpose

    Direct-expressing SYFP2 AAV Virus

    Depositor

    Allen Institute for Brain Science, Boaz Levi

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Promoter

    MinRho

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. hNav1.8_pcDNA3.1

    Plasmid

    #182847

    Purpose

    Expresses hNav1.8 in mammalian cells

    Depositor

    Paul DeCaen

    Article

    McCollum et al Cell Rep. 2022 Aug 23;40(8):111248. doi:

    Insert

    SCN10A (SCN10A Human)
    

    Expression

    Mammalian

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pZE27GFP

    Plasmid

    #75452

    Purpose

    Plasmid for constitutive expression of gfp

    Depositor

    James Collins

    Article

    Cameron et al Nat Biotechnol. 2014 Dec;32(12):1276-81.

    Insert

    gfp
    

    Expression

    Bacterial

    Promoter

    placIq promoter

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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21. AAVS1-TRE3G-EGFP

    Plasmid

    #52343

    Purpose

    AAVS1 donor vector for inducible gene expression

    Depositor

    Su-Chun Zhang

    Article

    Qian et al Stem Cells. 2014 Feb 4. doi: 10.1002/ste

    Insert

    EGFP
    

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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22. pSFFV_sfCherry2(1-10)

    Plasmid

    #82603

    Purpose

    Lentiviral vector for constitutive expression of sfCherry2(1-10)

    Depositor

    Bo Huang

    Article

    Feng et al Nat Commun. 2017 Aug 29;8(1):370. doi: 1

    Insert

    sfCherry2(1-10)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    changed Glu 118 to Gln, changed Thr 128 to Ile

    Promoter

    SFFV

    Available Since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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23. pC1-SypHer3s

    Plasmid

    #108118

    Purpose

    Ratiometric pH probe with enhanced brightness for mammalian cells expression.

    Depositor

    Vsevolod Belousov

    Article

    Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

    Insert

    SypHer3s
    

    Tags

    ratiometric YFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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24. ALDH18A1

    Plasmid

    #155402

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    ALDH18A1 (ALDH18A1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. APEX2-NLS

    Plasmid

    #124617

    Purpose

    proximity biotinylation

    Depositor

    Alice Ting

    Article

    Kaewsapsak et al Elife. 2017 Dec 14;6. doi: 10.7554/eLife

    Insert

    APEX2-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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26. TFORF3165

    Plasmid

    #144641

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TRIM28 (TRIM28 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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27. pIVT-eeBxb1

    Plasmid

    #232748

    Purpose

    IVT template for making modRNA encoding eeBxb1

    Depositor

    Kate Galloway

    Article

    Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Bxb1
    

    Use

    In vitro transcription

    Tags

    HA and SV40 NLS

    Mutation

    V74A, E229K, V375I

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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28. hCas9

    Plasmid

    #41815

    Purpose

    Expresses human codon optimized Cas9 nuclease for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    Cas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    human codon-optimized

    Promoter

    CMV

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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29. Cav1-GFP

    Plasmid

    #14433

    Depositor

    Ari Helenius

    Article

    Tagawa et al J Cell Biol. 2005 Aug 29. 170(5):769-79.

    Insert

    Caveolin-1 (CAV1 Canis familalis)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    March 23, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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30. L13-Arl13bGFP

    Plasmid

    #40879

    Purpose

    3rd generation transfer vector

    Depositor

    Tamara Caspary

    Article

    Larkins et al Mol Biol Cell. 2011 Dec;22(23):4694-703.

    Insert

    Arl13b (Arl13b Mouse)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 29, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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