We narrowed to 23,142 results for: CRISPR

1. pU6-(BbsI)sgRNA_CAG-Cas9-venus-bpA

   Plasmid

   #86986

   Purpose

   For cloning and expression of sgRNA together with expression of a Cas9-Venus fusion protein

   Depositor

   Ralf Kuehn

   Article

   Yumlu et al Methods Mol Biol. 2019;1961:137-151. doi

   Insert

   Cas9-venus
   

   Use

   Tags

   Cas9-Venus fusion protein

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available Since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

2. pCAS9cd

   Plasmid

   #141252

   Purpose

   Constitutive expression of Cas9 for addressing two targets; Contains two guide RNA expression cassette with stuffer and KpnI-Pme1 restriction sites.

   Depositor

   Adam Bertl

   Article

   Degreif et al J Biol Methods. 2018 Sep 12;5(3):e98. do

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   TEF1 (Cas9), pSNR52 (gRNA)

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCRISPEY-GAL-Kan

   Plasmid

   #232099

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with KanMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCRISPEY-GAL-Hyg

   Plasmid

   #232098

   Purpose

   Yeast CEN plasmid for galactose-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with HygMX selection

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. NAX_CAGGS_CAS9_m4_KRAB-MeCP2_mcherry

   Plasmid

   #167902

   Purpose

   PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Insert

   dCas9-KRAB-MeCP2
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   D10A, D839A, H840A, N863A

   Promoter

   Available Since

   Sept. 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCRISPEY-Z3-Nat

   Plasmid

   #232100

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available Since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCas9-EcRT-Z3-Hyg

   Plasmid

   #232095

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. LbdCas12a VPR

   Plasmid

   #188513

   Purpose

   Expresses FLAG tagged LbdCas12a VPR

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   dCas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   EF1a

   Available Since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYPQAT03

   Plasmid

   #223375

   Purpose

   T-DNA vector for SpCas9 mediated mutagenesis for dicot plants; NGG PAM; Cas9 was driven by 2x35s and the sgRNA was driven by AtU3 promoter; Kanamycin for plants selection.

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

   Insert

   2x35s-SpCas9-AtU3-gRNA scaffold
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 10, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pM122: pAAV-EFS-CasRx

    Plasmid

    #166870

    Purpose

    AAV vector for expressing CasRx

    Depositor

    Guei-Sheung Liu

    Article

    Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

    Insert

    RfxCas13d
    

    Use

    AAV and CRISPR

    Tags

    HA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHAGE-TO-nls-st1dCas9-3nls-3XGFP-nls

    Plasmid

    #64112

    Purpose

    Expression of St1 dCas9-3XGFP in mammalian cells

    Depositor

    Thoru Pederson

    Article

    Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

    Insert

    St1 dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3XsfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-TO

    Available Since

    April 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMH-Cas9-gate

    Plasmid

    #113742

    Purpose

    Gateway destination vector encoding Maize ubiquitin promoter driving Cas9 and 35S promoter driving hygromycin for selection in plants.

    Depositor

    Magdalena Bezanilla

    Article

    Mallett et al Plant Direct. 2019 Sep 12;3(9):e00168. d

    Insert

    Cas9
    

    Use

    CRISPR; Gateway destination vector

    Tags

    Expression

    Mutation

    Promoter

    Z. Mays ubiquitin promoter, 35S promoter

    Available Since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFYF1328 EGFP Site#3

    Plasmid

    #47513

    Purpose

    human gRNA expression vector targeting EGFP

    Depositor

    Keith Joung

    Article

    Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

    Insert

    gRNA-EGFP site 3
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDM026

    Plasmid

    #216808

    Purpose

    Vector for Aspergillus CRISPR-Cas9 genetic engineering with Golden Gate cloning drop-out cassette for protospacer insertion and pyrG selectable marker.

    Depositor

    Ken Sawin

    Article

    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do

    Type

    Empty backbone

    Use

    CRISPR; Fungal expression

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSET-dCas9-actII-4-NT-S1

    Plasmid

    #110185

    Purpose

    Repression of gene expression in Streptomyces by CRISPRi

    Depositor

    Yinhua Lu

    Article

    Zhao et al Biotechnol J. 2018 Jun 3. doi: 10.1002/b

    Inserts

    dCas9
    sgRNA
    

    Use

    CRISPR; I

    Tags

    Expression

    Bacterial

    Mutation

    D10A, H840A

    Promoter

    J23119 and ermE*p

    Available Since

    July 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKM126

    Plasmid

    #105134

    Purpose

    Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

    Depositor

    Joseph Sorg

    Article

    McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

    Inserts

    Upstream & Downstream pyrE deletion region
    tetR
    Cas9
    gRNA
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXEE401BG

    Plasmid

    #91717

    Purpose

    CRISPR/Cas9-mediated genome editing in Arabidopsis, Bar and glyphosate-resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. SETMAR-Cas9

    Plasmid

    #186701

    Purpose

    Plasmid encoding SETMAR-Cas9 fusion under CMV promoter

    Depositor

    Jennifer Doudna

    Article

    Chen et al Proc Natl Acad Sci U S A. 2022 Dec 6;119

    Insert

    SETMAR-Cas9
    

    Use

    CRISPR

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    July 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcrVIB1

    Plasmid

    #184566

    Purpose

    Inducible expression of anti-CRISPR protein AcrVIB1 in bacteria.

    Depositor

    Chase Beisel

    Article

    Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

    Insert

    anti-CRISPR protein inhibiting Cas13b nucleases
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available Since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pYPQ154

    Plasmid

    #69305

    Purpose

    Gateway entry vector with AteCas9 (Arabidopsis codon optimized)

    Depositor

    Yiping Qi

    Article

    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

    Insert

    AteCas9 (Arabidopsis codon optimized)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    NLS fusion

    Promoter

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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