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Showing: 521 - 560 of 4181 results

  9. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

    Plasmid
    #202569
    Purpose
    Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR
    Depositor
    Liam Holt
    Article
    Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75
    Insert
    LINE-1
    Use
    Tags
    GFP-AI retrotransposition reporter and HaloTag7 o…
    Expression
    Mammalian
    Mutation
    Changed ORF1p Arginine 261 to Alanine
    Promoter
    Available since
    April 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    202569-plasmid-map-sequence-id-429223

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  14. pZA-GFP-R5

    Plasmid
    #106256
    Purpose
    Constitutively expresses GFP fused with silaffin-R5 silica affinity peptide
    Depositor
    Urartu Seker
    Article
    Olmez et al ACS Omega. 2018 Jan 31;3(1):585-594. doi
    Insert
    Green fluorescent protein
    Use
    Tags
    Added silaffin R5 peptide to C terminus of GFP af…
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 9, 2019
    Availability
    Academic Institutions and Nonprofits only
    106256-plasmid-map-sequence-id-232729

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  16. p667-UBC-AAK1-V5-TurboID_IDG-K

    Plasmid
    #135256
    Purpose
    Gateway destination clone of AAK1 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics
    Depositor
    Ben Major
    Insert
    AAK1-V5-TurboID (AAK1 Human)
    Use
    Lentiviral; Gateway destination
    Tags
    V5-TurboID
    Expression
    Mammalian
    Mutation
    Promoter
    Ubiquitin
    Available since
    April 15, 2020
    Availability
    Academic Institutions and Nonprofits only
    135256-plasmid-map-sequence-id-267910

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  17. pDGE658

    Plasmid
    #153256
    Purpose
    M3 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.
    Depositor
    Johannes Stuttmann
    Article
    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1
    Type
    Empty backbone
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Aug. 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    153256-plasmid-map-sequence-id-305915

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  26. pSEVA47_dCas9-GFP_AAN_4

    Plasmid
    #231415
    Purpose
    This AND-AND-NOT-gate plasmid expresses dCas9 from a constitutive promoter. Its sgRNAs X & Y are repressible by sgRNAs A & B, respectively. Its GFP gene is repressible by any of sgRNAs X, Y, and C.
    Depositor
    Manish Kushwaha
    Article
    Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.
    Inserts
    dCas9
    GFP
    sgRNA-X
    sgRNA-Y
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    231415-plasmid-map-sequence-id-461754

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  35. FnRPR9-10

    Plasmid
    #182568
    Purpose
    Human fibronectin fragment, type-III repeats 9-10, R1374A, P1376A, R1379A mutant
    Depositor
    Timothy Springer
    Article
    Su et al J Biol Chem. 2022 Sep;298(9):102323. doi
    Insert
    Human fibronectin fragment, type-III repeats 9-10
    Use
    Tags
    His and TEV cleavage site after His tag
    Expression
    Bacterial
    Mutation
    R1374A, P1376A, R1379A
    Promoter
    Available since
    Oct. 11, 2022
    Availability
    Academic Institutions and Nonprofits only
    182568-plasmid-map-sequence-id-357182

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Showing: 521 - 560 of 4181 results