We narrowed to 4,893 results for: PIR;

1. SepOTSν

   Plasmid

   #68294

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EF-Sep21, 5xtRNA-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. SepOTSβ

   Plasmid

   #68283

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 1xtRNA-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pTHSSd_40

   Plasmid

   #59963

   Purpose

   High core fragment expression without SynZIP

   Depositor

   Christopher Voigt

   Article

   Segall-Shapiro et al Mol Syst Biol. 2014 Jul 30;10(7):742.

   Insert

   core fragment lacking SynZIP
   

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   PJ23105

   Available Since

   Oct. 15, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCIwt-PRM-Wasabi

   Plasmid

   #195553

   Purpose

   Always-on expression of mWasabi reporter with transcription activation by wildtype CI

   Depositor

   Mikhail Shapiro

   Article

   Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

   Inserts

   mWasabi
   CIwt
   

   Tags

   6x His and AANDENYADAS degradation tag

   Expression

   Bacterial

   Available Since

   March 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. SepOTSι

   Plasmid

   #68290

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EFSep21, 2xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SepOTSη

   Plasmid

   #68288

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS9/EF-Sep21, 2xtRNA-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. SepOTSζ

   Plasmid

   #68287

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 2xtRNA-Sep
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. SepOTSδ

   Plasmid

   #68285

   Purpose

   Phosphoserine orthogonal translation system

   Depositor

   Jesse Rinehart

   Article

   Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

   Insert

   SepRS/EF-Sep, 1xtRNA-Sep-A37
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Sept. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRS156

   Plasmid

   #182707

   Purpose

   CRISPRa plasmid for Candida albicans (dCas9-VPR and sgRNA cloning site)

   Depositor

   Rebecca Shapiro

   Article

   Gervais et al G3 (Bethesda). 2022 Dec 1:jkac301. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Nov. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. SepOTSλ

    Plasmid

    #68292

    Purpose

    Phosphoserine orthogonal translation system (preferred variant)

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SepRS9/EFSep21, 4xtRNA-Sep-A37
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. CAP-AAV.FUS.3

    Plasmid

    #222333

    Purpose

    AAV.FUS.3 Rep-Cap plasmid for production of AAV.FUS.3, a capsid with tropism for CNS neurons upon focused ultrasound mediated delivery.

    Depositor

    Jerzy Szablowski, Mikhail Shapiro

    Article

    Li et al Nat Commun. 2024 Jun 10;15(1):4924. doi:

    Insert

    AAV9 modified with 7-amino acid insertion between AA position 588 and 589 of VP1
    

    Use

    AAV

    Promoter

    p41

    Available Since

    Aug. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pTcI39-switch

    Plasmid

    #195557

    Purpose

    Temperature state switch expressing low-temperature mWasabi and high-temperature mRFP1

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mRFP1
    mWasabi
    TcI39
    

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a_T7-ARG2

    Plasmid

    #106474

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG2) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMetTU1-A_Sv5K_araC-PBAD_MCD1_MegaB-S9G-R31L-R85L_MegaRNFGLSKJTU_Bba-B0015

    Plasmid

    #202025

    Purpose

    Expresses Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Bacillus megaterium ARG cluster (GvpB S9G-R31L-R85L mutant)
    

    Expression

    Bacterial

    Mutation

    GvpB S9G-R31L-R85L

    Available Since

    July 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. SupD v2

    Plasmid

    #198598

    Purpose

    Plasmid encodes two copies of the suppressor tRNA SupD that decodes amber (UAG) codons as serine.

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    SupD
    

    Expression

    Bacterial

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTD103-ARG1

    Plasmid

    #106475

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    pLux

    Available Since

    Aug. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a_T5-ARG1

    Plasmid

    #106476

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-I48V_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202023

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-I48V mutant)
    

    Expression

    Bacterial

    Mutation

    GvpA T6A-I48V

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. E17TAG GFP zeo resistance

    Plasmid

    #69118

    Purpose

    For E17TAG GFP reporter expression in non-zeocin resistant strains

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    E17TAG GFP
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    E17 to TAG

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Beta lactamase S68TAG

    Plasmid

    #68305

    Purpose

    Beta lactamase S68TAG gene

    Depositor

    Jesse Rinehart

    Article

    Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

    Insert

    S68TAG beta lactamase
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Sept. 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET28_LmFPPS

    Plasmid

    #186322

    Purpose

    pET-28a vector (Novagen) (encoding an N-terminal His tag), to transform Escherichia coli BL21 (DE3) cells

    Depositor

    Sandra Gabelli

    Article

    Aripirala et al Acta Crystallogr D Biol Crystallogr. 201

    Insert

    FPPS
    

    Tags

    His Tag

    Expression

    Bacterial

    Available Since

    Oct. 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTcI39-PRM-wasabi

    Plasmid

    #195555

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI39
    

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pMetTU1-A_Sv5K_araC-PBAD_MCD1_AnaA-T6A-L40A_AnaCNJKFGW_Bba-B0015

    Plasmid

    #202024

    Purpose

    Expresses Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant) in E. coli

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al bioRxiv 2024.03.30.587094

    Insert

    Anabaena flos-aquae ARG cluster (GvpA T6A-L40A mutant)
    

    Expression

    Bacterial

    Mutation

    GvpA T6A-L40A

    Available Since

    Aug. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pAP01

    Plasmid

    #186260

    Purpose

    sfGFP under light light responsive PEL222 promoter

    Depositor

    Yongku Cho

    Article

    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35

    Insert

    sfGFP
    

    Expression

    Bacterial

    Promoter

    PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTA…

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pJK-caCas9-NatMX-Neut5L

    Plasmid

    #89576

    Purpose

    caCas9 integrating vector into the Neut5L locus

    Depositor

    George Church

    Article

    Shapiro et al Nat Microbiol. 2018 Jan;3(1):73-82. doi:

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Yeast

    Available Since

    Oct. 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pEF1alpha-rtTA-Antares

    Plasmid

    #197587

    Purpose

    Expresses TET-On reverse transactivator labeled with Antares

    Depositor

    Mikhail Shapiro

    Article

    Hurt et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    rtTA-P2A-Antares
    

    Tags

    Antares

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3.1_GEM7-Halo-FRB

    Plasmid

    #197058

    Purpose

    Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

    Depositor

    Julia Mahamid

    Article

    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

    Insert

    Encapsulin
    

    Tags

    FRB and HaloTag

    Expression

    Mammalian

    Mutation

    Codon-optimised for human cell expression

    Available Since

    Sept. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. SQT1665

    Plasmid

    #78744

    Purpose

    Human expression plasmid for LbCpf1-NLS-3xHA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

    Insert

    mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
    

    Use

    CRISPR

    Tags

    NLS-3xHA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 16, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGBW-m4046785

    Plasmid

    #145684

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

30. pHYP-NPC-10H

    Plasmid

    #162789

    Purpose

    SARS-CoV-2 Nucleocapsid Protein expression in E.coli

    Depositor

    Ivan Vorobiev

    Article

    Kolesov et al PeerJ. 2022 Jan 7;10:e12751. doi: 10.771

    Insert

    Nucleocapsid phosphoprotein [Severe acute respiratory syndrome coronavirus 2] (N Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    10xHis

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBEST_OR2-OR1-Pr-UTR1_ClpP-T500

    Plasmid

    #153302

    Purpose

    Expresses ClpP protease in the cell-free TX-TL system

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    clpP (clpP Escherichia coli)
    

    Expression

    Bacterial

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pMA-Thermal_CTLA-4

    Plasmid

    #199751

    Purpose

    Thermal expression of CTLA-4

    Depositor

    Mikhail Shapiro

    Article

    Abedi et al Nat Commun. 2022 Mar 24;13(1):1585. doi:

    Insert

    CTLA-4 (Ctla4 Mouse)
    

    Expression

    Bacterial

    Available Since

    May 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGBW-m4046810

    Plasmid

    #149251

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available Since

    May 11, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

34. pET28a-T5-ASG_ClpXP

    Plasmid

    #153299

    Purpose

    Expresses acoustic sensor genes or ClpXP-sensitive gas vesicles in E. coli Nissle 1917

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    ASG
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T5

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTetR-BCD2-Ana GvpC

    Plasmid

    #153298

    Purpose

    Expresses wild-type gas vesicle protein C (GvpC) from Anabaena flos-aquae

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Chem Biol. 2020 Sep;16(9):988-996. d

    Insert

    Gas vesicle protein C
    

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGG-PIP31

    Plasmid

    #196769

    Purpose

    Contains RBOHD promoter

    Depositor

    Marc Somssich

    Article

    Calabria et al J Exp Bot. 2025 Jan 17:erae516. doi: 10.

    Insert

    RESPIRATORY BURST OXIDASE HOMOLOGUE D (RBOHD Mustard Weed)
    

    Use

    Greengate (gg) entry vector

    Available Since

    Dec. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pTcI38-PRM-wasabi

    Plasmid

    #195554

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI38
    

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pST39-pNL29

    Plasmid

    #91696

    Purpose

    Expresses Mega GVs in E.Coli

    Depositor

    Mikhail Shapiro

    Article

    Lakshmanan et al Nat Protoc. 2017 Oct;12(10):2050-2080. d

    Insert

    Mega GV gene cluster (from pNL29) in pST39 backbone
    

    Tags

    No tags

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Oct. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. WT GBA pcDNA3.1

    Plasmid

    #188580

    Purpose

    Ectopic expression of wild-type GBA

    Depositor

    Anthony Schapira

    Article

    Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

    Insert

    GBA (GBA1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

    Plasmid

    #221075

    Purpose

    SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

    Depositor

    Mikhail Shapiro

    Article

    Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

    Insert

    ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge