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We narrowed to 18,247 results for: multi

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Showing: 261 - 280 of 18247 results

  1. pUC119-gRNA

    Plasmid
    #52255
    Purpose
    Can use a PCR template to assemble new desired guide RNA. Contains an Arabidopsis U6 promoter to drive guide RNA (targeting AtPDS3 gene target site 1) expression with a TTTTTT as terminator.
    Depositor
    Jen Sheen
    Publication
    Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d
    Insert
    guide RNA targeting AtPDS3 (PDS3 Mustard Weed)
    Use
    CRISPR; Plant expression
    Promoter
    Arabidopsis U6 polymerase III promoter
    Available since
    March 20, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    52255-plasmid-map-sequence-id-80847

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  4. pCBC-DT2T3

    Plasmid
    #50591
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Insert
    T2, gRNA scaffold, U6-29t plus, U6-1p, T3
    Use
    CRISPR; Pcr template
    Expression
    Plant
    Available since
    Dec. 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    50591-plasmid-map-sequence-id-244476

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  6. pMA3790

    Plasmid
    #70110
    Purpose
    mtDNA elimination/production of rho-0 cells
    Depositor
    Mikhail Alexeyev
    Publication
    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    EGFP
    hUNG1 Y147A
    Neo
    Tags
    None, human OTC mitochondrial targeting sequence,…
    Expression
    Mammalian
    Mutation
    Y147A, aa's 1-77 removed
    Promoter
    CMV, EF1a, and SV40 and native
    Available since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    70110-plasmid-map-sequence-id-399654

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  7. CLYBL-TO-hNGN2-BSD-mApple

    Plasmid
    #124229
    Purpose
    Targets the human CLYBL safe harbor locus for integration of dox-inducible NGN2 for differentiation of iPSCs into cortical neurons. Contains a floxed BSD-NLS-mApple selection cassette.
    Depositor
    Michael Ward
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    NGN2 (NEUROG2 Human)
    Expression
    Mammalian
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    124229-plasmid-map-sequence-id-476962

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  8. pLVX-UbC-rtTA-Ngn2:2A:Ascl1

    Plasmid
    #127289
    Purpose
    all-in-one inducible LV expression construct for Ngn2:2A:Ascl1 (aka UNA; for direct neuronal conversion)
    Depositor
    Fred Gage
    Publication
    Herdy et al Elife. 2019 May 17;8. pii: 41356. doi: 1
    Inserts
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Ngn2:2A:Ascl1 (ASCL1, NEUROG2 Human)
    Use
    Lentiviral
    Tags
    Ngn2 and Ascl1 linked via 2A
    Expression
    Mammalian
    Available since
    Dec. 4, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    127289-plasmid-map-sequence-id-308425

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  10. ColorfulCell

    Plasmid
    #62449
    Purpose
    MXS_chaining vector with 6 cassettes
    Depositor
    Pierre Neveu
    Publication
    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    CMV::GTS-Citrine-bGHpA
    CMV::Lyn-Ceruleanx3-bGHpA
    CMV::NLS-TagBFPx3-bGHpA
    CMV::MTS-AzamiGreen-bGHpA
    CMV::iRFP670-SKL-bGHpA
    CMV::ETS-mCherry-KDEL-bGHpA
    Use
    Synthetic Biology
    Available since
    May 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62449-plasmid-map-sequence-id-209509

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  11. pRT029

    Plasmid
    #127969
    Purpose
    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127969-plasmid-map-sequence-id-398131

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  14. pLSC-5

    Plasmid
    #62889
    Purpose
    Lenti vector for expression of inducible split Cas9
    Depositor
    Feng Zhang
    Publication
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Inserts
    SpCas9(574-1368)
    SpCas9(2-573)
    Use
    CRISPR and Lentiviral
    Tags
    FFBP12, FRB, NES PTK2, NLS SV40, and P2A
    Expression
    Mammalian
    Mutation
    *see comment below
    Promoter
    EFS
    Available since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62889-plasmid-map-sequence-id-105912

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  15. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Publication
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    51760-plasmid-map-sequence-id-392672

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  18. HypoxCR

    Plasmid
    #59946
    Purpose
    A lentiviral dual fluorescent protein reporter, HypoxCR, detects hypoxic [hypoxia-inducible factor (HIF) active] and/or cycling cells.
    Depositor
    Anne Le
    Publication
    Le et al Proc Natl Acad Sci U S A. 2014 Aug 11. p
    Inserts
    GFP-Pest
    mCherry-Geminin
    Use
    Lentiviral
    Tags
    FLAG and PEST
    Expression
    Mammalian
    Promoter
    CMV and min CMV
    Available since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    59946-plasmid-map-sequence-id-228094

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  19. pLenti-CaMKIIa-hChR2(H134R)-mCherry-WPRE

    Plasmid
    #20943
    Purpose
    3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by CaMKIIa promoter for optogenetic activation
    Depositor
    Karl Deisseroth
    Publication
    Zhang et al Nature. 2007 Apr 5. 446(7136):633-9.
    Insert
    channelrhodopsin-2
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    H134R
    Promoter
    CaMKIIa
    Available since
    July 9, 2009
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    20943-plasmid-map-sequence-id-171248

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Showing: 261 - 280 of 18247 results