179,521 results
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Plasmid#106259PurposeExpresses MPH co-activator from the CAG promoter and one U6-driven dgRNA in AAV backboneDepositorAvailable sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by
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pGL3 3 kb prom. CD274
Plasmid#107004PurposeModified Luciferase expression vectorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCD274 (CD274 Human)
UseLuciferaseExpressionMammalianAvailable sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV.CMV.ffLuciferase.SV40
Plasmid#105532PurposeAAV expression of firefly luciferase from CMV promoterDepositorHas serviceAAV1 and AAV8InsertLuciferase
UseAAV and LuciferaseExpressionMammalianPromoterCMVAvailable sinceFeb. 2, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
T7-CMVtrans-FFLuc-polyA
Plasmid#101156PurposeTemplate for T7 RNAP production of RNA transcript for expression of firefly luciferase in a eukaryotic mammalian cell free lysate.DepositorInsertFirefly luciferase
UseLuciferasePromoterT7 promoterAvailable sinceJan. 22, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PB-SAM
Plasmid#102559PurposePiggybac transposon vector encoding dCAS9-VP64 and MS2-P65-HSF1 activator helper complex.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsMS2-P65-HSF1_T2A_Hygro
dCAS9(D10A, N863A)-VP64_T2A_Blast
UseCRISPR; Piggybac transposonExpressionMammalianMutationD10A and N863A in Cas9 and N55K in MS2PromoterCAG and EF1AAvailable sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CD
Plasmid#101921PurposeThe dCas9-TET1CD fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948).DepositorInsertdCas9-TET1CD
UseLentiviralExpressionMammalianAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pINDUCER-21-SPI1
Plasmid#97039PurposeExpresses SPI1 in mammalian cellsDepositorAvailable sinceSept. 5, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLX304 Luciferase-V5 blast
Plasmid#98580PurposeLentiviral vector for constitutive expression of Luciferase with C-terminal V5 tagDepositorInsertLuciferase
UseLentiviralTagsV5ExpressionMammalianAvailable sinceAug. 15, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-FLAG-SF2
Plasmid#99021PurposeMammalian expression of SF2DepositorAvailable sinceJuly 28, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET42b-BE3
Plasmid#87437PurposeExpresses BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET42b-HF-BE3
Plasmid#87438PurposeExpresses HF-BE3-NLS with an N-terminal His Tag (His6) for bacterial expressionDepositorAvailable sinceJune 13, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-Rab27B
Plasmid#89447PurposeExpression of GFP-tagged human Rab27B in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthuman Rab27B (RAB27B Human)
TagseGFPExpressionMammalianMutationwild typePromoterCMVAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HIV-1 Gag-mCherry
Plasmid#85390PurposeVisualization marker for viral core to measure cellular uptakeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHIV-1 gag
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceJan. 23, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmScarlet-i_Giantin_C1
Plasmid#85050PurposeIn vivo visualization of the Golgi apparatus (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGiantin (GOLGB1 Human)
ExpressionMammalianMutationaa 3131-3259PromoterCMVAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hGli1 flag3x
Plasmid#84922Purposemammalian expression of Gli1DepositorAvailable sinceNov. 14, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK5-HA-YFP-rictor
Plasmid#73387PurposeExpresses HA and YFP tagged RictorDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRPTOR Independent Companion Of MTOR, Complex 2 (RICTOR Human)
TagsHA and YFPExpressionMammalianPromoterCMVAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-N-CretrcintG
Plasmid#69570PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for rAAV production and expression in mammalian cellsDepositorHas serviceAAV1Insertkozak consensus - N-CretrcintG
UseAAV and Synthetic BiologyExpressionMammalianPromoterEF1aAvailable sinceOct. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-Ii_VSVG-SBP-mCherry
Plasmid#65301Purposesynchronize trafficking of VSVG from the ER (RUSH system)DepositorInsertVSV-G
TagsStreptavidin Binding Protein (SBP) and mCherryExpressionMammalianPromoterCMVAvailable sinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEYFP-Sec24C
Plasmid#66608PurposeYFP fusion of Sec24CDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec24C (SEC24C Human)
ExpressionMammalianAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA-dCas9-FLp300
Plasmid#61356Purposeencodes S. pyogenes dCas9 with c-terminal fusion of FL human p300 (aa 2-2414) driven by CMV promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertS.pyogenes dCas9 with c-terminal full length human p300 (aa 2-2414) (EP300 Human, Synthetic, S. pyogenes)
UseCRISPR and Synthetic BiologyTagsFlag, HA, and SV40 NLSExpressionMammalianMutationD10A; H840A in S.pyogenes Cas9PromoterCMVAvailable sinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSBtet-Neo
Plasmid#60509PurposeSB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of rtTA and neomycin resistance geneDepositorTypeEmpty backboneUseTransposonExpressionMammalianPromoterTCE & RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-RNF4/pRK5
Plasmid#59743PurposeExpresses RNF4 in mammalian cellsDepositorAvailable sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nanog-2A-mCherry
Plasmid#59995PurposeInserts mCherry immediately downstream of the Nanog coding regionDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNanog (Nanog Mouse)
UseMouse TargetingTags2A-mCherryAvailable sinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-HA-hIFITM1
Plasmid#58399PurposeExpressed human IFITM1 with an N-terminal HA tag in mammalian cellsDepositorAvailable sinceSept. 8, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Calnexin-N-14
Plasmid#55005PurposeLocalization: Endoplasmic Reticulum, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCalnexin (CANX Human)
TagsmCherryExpressionMammalianMutationI474N in CalnexinPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5 FRT/TO SF-HERC2 (ShB-R)
Plasmid#55613PurposeDox-inducible expression of full length, ShB-resistant, 3xFLAG/STREP tagged wild-type HERC2 in Flp-In TREx cellsDepositorInsert3xFLAG tagged HERC2 (WT) (HERC2 Human)
Tags3xFLAG and STREPExpressionMammalianMutationSilent mutations have been incorporated into the …Available sinceJuly 23, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pICSL11024 (pICH47732::NOSp-NPTII-OCST)
Plasmid#51144PurposeConfers KAN resistance in plantaDepositorInsertNOSp-NPTII-OCST
Available sinceFeb. 26, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-PIG-Cre
Plasmid#50935Purposeretroviral expression of Cre recombinaseDepositorPublicationInsertCre recombinase
UseCre/Lox and RetroviralTagsNLSExpressionMammalianAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCFD1-dU6:1gRNA
Plasmid#49408Purposeexpression of gRNA under control of the Drosophila U6:1 promoterDepositorInsertdU6-1:gRNA
UseCRISPRExpressionInsectPromoterdU6-1Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-Chili
Plasmid#48687Purpose3rd generation Lentiviral vector with bi-cistronic expression of dtomato and the gene of interest.DepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable sinceOct. 28, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV10/3xflag-Clock
Plasmid#47334Purposefull length clock in pCMV10 with 3x flag at the N-terminusDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertclock
Tags3x FlagExpressionMammalianPromoterCMVAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCMV-Luc
Plasmid#45968DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLuciferase
ExpressionMammalianPromoterCMVAvailable sinceAug. 14, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
hGFAP-fLuc
Plasmid#40589DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthGFAP promoter (GFAP Human)
UseLuciferaseTagsFirefly luciferaseMutationContains human GFAP promoter fragment (-2163 to +…PromoterGFAP promoterAvailable sinceSept. 20, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-EF1a-FLEX-TVA-mCherry
Plasmid#38044DepositorInsertAvian sarcoma and leukemia virus receptor 950 (CD320 quail, Synthetic)
UseAAV and Cre/LoxTagsmCherryExpressionMammalianPromoterEF1aAvailable sinceAug. 15, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLenti 4.1 Ex miR200c-141
Plasmid#35534Purpose3rd generation lentiviral vectorDepositorUseLentiviralTagsGFPExpressionMammalianPromoterCMVAvailable sinceApril 30, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-N1-human lyn–GFP
Plasmid#35958PurposeExpresses human lyn protein codon optimized for zebrafish expression.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertLYN (LYN Human)
TagsEGFPExpressionMammalianMutationwtPromotercmvAvailable sinceApril 27, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pECE-M2-BAIAP2 wt
Plasmid#31656DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertBAI1-associated protein 2 (BAIAP2 Human)
TagsM2ExpressionMammalianAvailable sinceApril 11, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
rAAV-syn::FLEX-rev::PSAML141F,Y115F:GlyR-IRES-GFP
Plasmid#32481PurposeCre-dependent expression of PSAM-GlyR (L141F,Y115F) neuronal inhibitor, with IRES-EGFP markerDepositorInsertPSAML141F,Y115F-GlyR
UseAAV and Cre/Lox; Adeno-associated virus, flex swi…TagsIRES-EGFPPromoterSynapsinAvailable sinceJan. 13, 2012AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP- RPL26
Plasmid#31980DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRPL26 (RPL26 Human)
TagsGFPExpressionMammalianAvailable sinceSept. 19, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLv-CMV-MyoD-ER(T)
Plasmid#26809DepositorInsertMyoD-ER(T) (Myod1 Mouse)
UseLentiviralAvailable sinceSept. 12, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by