We narrowed to 12,555 results for: LIC

1. pRSF_ApyADO

   Plasmid

   #226248

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD and the cytochrome P450 enzyme ApyO

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   ApyO
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pRSF_ApyAO

   Plasmid

   #226242

   Purpose

   Production of ApyA peptide modified by the cytochrome P450 enzyme ApyO

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyO
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRSF_ApyAD

   Plasmid

   #226241

   Purpose

   Production of ApyA peptide modified by the B12-rSAM enzyme ApyD

   Depositor

   Wilfred van der Donk

   Article

   Nguyen et al ACS Cent Sci. 2024 Apr 11;10(5):1022-103

   Inserts

   His6_ApyA
   ApyD
   

   Use

   Tags

   Hexa-Histidine

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. p3E-WPRE-bGH-pA (JDW 1221)

   Plasmid

   #224527

   Purpose

   A Gateway compatible 3' entry clone containing a WPRE upstream of Bovine growth hormone polyA to stabilize mRN

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   WPRE-bGH
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CB2R-GFP

   Plasmid

   #208919

   Purpose

   encodes the human cannabinoid receptor 2, tagged with GFP

   Depositor

   Christian Gruber

   Article

   Tomašević et al J Biol Chem. 2024 Jun;300(6):107330. doi

   Insert

   CNR2 (CNR2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   MCV

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pME_Golgi-BFP_P2A_H2A_iRFP (JDW 1007)

   Plasmid

   #224490

   Purpose

   Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-iRFP (BFP golgi reporter and infrared RFP nuclear reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Golgi-mTagBFP-P2A-H2A-iRFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pME_Golgi-BFP_P2A_H2A_tdiRFP (JDW 1079)

   Plasmid

   #224491

   Purpose

   Gateway compatible middle entry clone containing GalT-mTagBFP-HA-P2A-H2A-tdiRFP (BFP golgi reporter and tandem infrared RFP nuclear reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Golgi-mTagBFP-P2A-H2A-tdiRFP
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pME-rox-nls-mCherry-V5-2xStop-rox (JDW 1167)

   Plasmid

   #224524

   Purpose

   A Gateway compatible middle entry clone containing a Rox flanked 3xNLS-mCherry-V5-2xStop cassette

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   rox-3xNLS-mCherry-V5-2xSTOP-rox
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p5E lexOP-c-fos min pro (JDW 1254)

   Plasmid

   #224477

   Purpose

   Gateway compatible 5' entry clone containing a Lex Operon upstream of a c-fos minimal promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LexOp
   

   Use

   Gateway cloning

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. p5E-dr_Unc (JDW 1365)

    Plasmid

    #224482

    Purpose

    Gateway compatible 5' entry clone containing the Zebrafish unc-503 promoter with a minimal c-fos promoter and b-globin intron

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Unc-503 promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pME-H2B-V5-n2StayGoldC4 (JDW 1354)

    Plasmid

    #224486

    Purpose

    Gateway compatible middle entry clone contianing Histone H2B fusion to V5-tagged (n2)StayGold(C4) (Nuclear StayGold fluorescent reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    H2B-V5-n2StayGoldC4
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pME-myr-mTagBFP2-Flag (JDW 1183)

    Plasmid

    #224492

    Purpose

    Gateway compatible middle entry clone containing a Myristoylated mTagBP2 with a c-terminal Flag tag (cell membrane BFP marker)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    myr-mTagBFP2-Flag
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pME-Lifeact-mCherry-3xHA (JDW 1321)

    Plasmid

    #224493

    Purpose

    Gateway compatible middle entry clone containing Lifeact-mCherry (RFP F-actin reporter)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Lifeact-mCherry-3xHA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pME TAEL2.0-5xC120-minpro (JDW 1109)

    Plasmid

    #224507

    Purpose

    Gateway compatible middle entry clone containing TAEL2.0 transcription factor and 5xC120 response element (All in one binary TAEL and effector domain clone)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    TAEL2.0-5xC120
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pME-TAEL (JDW 1324)

    Plasmid

    #224508

    Purpose

    Gateway compatible middle entry clone containing TAEL2.0 transcription factor for making binary TAEL system vectors (generating a tissue specific driver)

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    TAEL
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pME-Myc-BioID2-MCS2 (JDW 1209)

    Plasmid

    #224511

    Purpose

    A Gateway compatible middle clone with a Myc-tagged BIOID2 construct followed by a flexible linker and an MCS

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Myc-BioID2
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pME nls EGFP-Cre (JDW 1329)

    Plasmid

    #224515

    Purpose

    A Gateway compatible middle entry clone containing a NLS-EGFP-Cre fusion

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    nls-EGFP-Cre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pME iCre-I (JDW 1203)

    Plasmid

    #224520

    Purpose

    A Gateway compatible middle entry clone containing a loxP flanked, self inactivating Cre that contains an intron to prevent recombination in bacteria

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    iCre-I
    

    Use

    Cre/Lox

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p3E-IRES-H2A-mCherry-SV40-pA (JDW 1256)

    Plasmid

    #224532

    Purpose

    A Gateway compatible 3' entry clone containing an IRES H2A mCherry followed by a polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    IRES-H2A-mCherry-SV40pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p3E EFS-rtTA/rtTA3 (JDW 1214)

    Plasmid

    #224533

    Purpose

    A Gateway compatible 3' entry clone containing the Human EFS promoter driving rtTA-3rd generation transactivator

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    rtTA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    EFS

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p3E-Actin-Vhh-mNeonGreen-HA (JDW 1225)

    Plasmid

    #224534

    Purpose

    A Gateway compatible 3' entry clone containing an Actin nanobody fused to mNeonGreen with a c-terminal HA tag

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    Actin-Vhh-mNeonGreen-HA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p5E 5X C120 c-fos min pro (JDW 1241)

    Plasmid

    #224544

    Purpose

    A Gateway compatible 5' entry clone containing 5 TAEL transcription factor binding sites and a minimal c-Fos promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    5x-C120-c-Fos promoter
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. p3E-WPRE-SV40-pA (JDW 922)

    Plasmid

    #224545

    Purpose

    A Gateway compatible 3' entry clone containing a woodchuck herpes simplex virus regulatory element (WPRE) to stabilize mRNA upstream of an SV40 polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    WPRE-SV40-pA
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEMHE:hTRAAK(Q258K)

    Plasmid

    #224763

    Purpose

    In vitro transcription for Oocyte expression of hTRAAK(Q258K)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 4 (KCNK4 Human)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Q258K

    Promoter

    T7

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pGEMHE:mTREK-1

    Plasmid

    #224743

    Purpose

    In vitro transcription for Oocyte expression of mTREK-1

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily K member 2 (Kcnk2 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pGEMHE:hTRAAK

    Plasmid

    #224761

    Purpose

    In vitro transcription for Oocyte expression of hTRAAK

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 4 (KCNK4 Human)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEMHE:hTRAAK(S118C)

    Plasmid

    #224762

    Purpose

    In vitro transcription for Oocyte expression of hTRAAK(S118C)

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 4 (KCNK4 Human)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    S118C

    Promoter

    T7

    Available since

    Sept. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEMHE:mTRAAK

    Plasmid

    #224765

    Purpose

    In vitro transcription for Oocyte expression of mTRAAK

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 4 (Kcnk4 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pPicZ:mTREK-1_cryst_I110D

    Plasmid

    #209640

    Purpose

    P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

    Depositor

    Dan Minor

    Article

    Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

    Insert

    Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
    

    Use

    Tags

    SNS- 3C_cleavage_site-TAAA-GFP

    Expression

    Yeast

    Mutation

    aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

    Promoter

    Available since

    Sept. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pBK1302-AAV-EFSNC-dCjCas9-KRAB

    Plasmid

    #223147

    Purpose

    Expression of KRAB with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pBK1312-AAV-EFSNC-dSaCas9-KRAB

    Plasmid

    #223157

    Purpose

    Expression of KRAB with dSaCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pBK2043-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9

    Plasmid

    #223163

    Purpose

    2nd gen. AAV backbone for dSaCas9 (endonuclease dead Cas9 from Staphylococcus aureus) and Sa-gRNA Scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    Available since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pT3TS-spCas9 ABE-Umax-ex1

    Plasmid

    #222141

    Purpose

    Adenine base editor with modified TadA variant for extended window for A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    Aug. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcs2+spCas9 ABE-Umax-nanos

    Plasmid

    #222142

    Purpose

    Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 with nanos UTR
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    SP6

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMXS-IRES-Blast coUCK2 D62A

    Plasmid

    #211530

    Purpose

    Expresses codon optimized catalytic mutant UCK2

    Depositor

    Richard Possemato

    Article

    Nam et al Nat Metab. 2024 Dec;6(12):2338-2353. doi

    Insert

    UCK2 D62A (UCK2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    D62A (catalytic mutant)

    Promoter

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pT3TS-spCas9 ABE-Umax-ex2-rest1

    Plasmid

    #222143

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pT3TS-spCas9 ABE-Umax-ex1-rest1

    Plasmid

    #222140

    Purpose

    Adenine base editor with modified TadA variant for extended window and restricted A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    T3

    Available since

    July 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pNeonGreen-C1-Nir1-631-894

    Plasmid

    #220089

    Purpose

    Truncated Nir1-LNS2 domain lacking N-terminal amphipathic helix; loses ability to bind PA

    Depositor

    Gerry Hammond

    Article

    Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

    Insert

    PITPNM3 (PITPNM3 Human)
    

    Use

    Tags

    NeonGreen-GGSGG

    Expression

    Mammalian

    Mutation

    amino acids 631-894

    Promoter

    CMV

    Available since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pMRX-IP-hWIPI2B-3×FLAG

    Plasmid

    #215510

    Purpose

    Expresses FLAG tagged human WIPI2B.

    Depositor

    Noboru Mizushima

    Article

    Shimizu et al Hum Mol Genet. 2023 Aug 7;32(16):2623-26

    Insert

    WD-repeat protein interacting with phosphoinositides 2B (WIPI2 Human)
    

    Use

    Retroviral

    Tags

    3×FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. NSP4-EGFP

    Plasmid

    #210378

    Purpose

    Expresses SARS-CoV-2 NSP4 fused with EGFP in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    NSP4 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

41. NSP2-Flag

    Plasmid

    #210344

    Purpose

    Expresses SARS-CoV-2 NSP2 in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SARS-CoV-2 NSP2 (ORF1ab Human, SARS-CoV-2 isolate Wuhan-Hu-1)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

42. pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

    Plasmid

    #213729

    Purpose

    Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

    Depositor

    Chaolin Zhang

    Article

    Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

    Insert

    bII spectrin (repeats 13-15) (Spnb3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pAAVS1-Neo-TNNT2-Blast

    Plasmid

    #214014

    Purpose

    Expression of cardiac troponin T

    Depositor

    Paul Burridge

    Article

    Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

    Insert

    TNNT2 (TNNT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. pAAVS1-Neo-TNNT2-Hygro

    Plasmid

    #214015

    Purpose

    Expression of cardiac troponin T

    Depositor

    Paul Burridge

    Article

    Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

    Insert

    TNNT2 (TNNT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pAAVS1-Blast-TNNT2-Zeo

    Plasmid

    #214018

    Purpose

    Expression of cardiac troponin T

    Depositor

    Paul Burridge

    Article

    Fetterman et al Cell Rep. 2024 May 28;43(5):114160. doi:

    Insert

    TNNT2 (TNNT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. SUMO-Twinkle 43-684

    Plasmid

    #205056

    Purpose

    TWNK protein expression

    Depositor

    Smita Patel

    Article

    Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

    Insert

    TWNK (TWNK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks amino acid from 1-42

    Promoter

    Available since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

47. NSP3C-mCherry

    Plasmid

    #210377

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with mCherry tag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    NSP3C (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. SEC61B-2Strep

    Plasmid

    #210375

    Purpose

    Expresses SEC61B fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    SEC61B (SEC61B Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. TMEM106B-2Strep

    Plasmid

    #210360

    Purpose

    Expresses TMEM106B fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    TMEM106B (TMEM106B Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. SUMO-Twinkle 360-684

    Plasmid

    #205055

    Purpose

    TWNK protein expression

    Depositor

    Smita Patel

    Article

    Johnson et al J Biol Chem. 2023 Jan;299(1):102797. doi

    Insert

    TWNK (TWNK Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Lacks N terminal amino acid from 1-359

    Promoter

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge