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We narrowed to 10,227 results for: T7

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pCellFree_G03 IL2

Plasmid

#67053

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

IL2 (IL2 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
BSA2_His

Plasmid

#202767

Purpose

bovine serum albumin design BSA2, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA2

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
BSA3_His

Plasmid

#202768

Purpose

bovine serum albumin design BSA3, in pET29b with C-terminal 6His-tag

Depositor

Sarel Fleishman

Article

Khersonsky et al J Mol Biol. 2023 Sep 1;435(17):168191. d

Insert

BSA1

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pPagP

Plasmid

#112125

Purpose

Bacterial expression of outer membrane protein PagP, wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

PagP (pagP E. coli)

Use

Tags

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-Venus-Emi1 (914)

Plasmid

#39854

Depositor

Jonathon Pines

Article

Di Fiore et al J Cell Biol. 2007 May 7;177(3):425-37.

Insert

Emi1 (FBXO5 Human)

Use

In vitro transcription

Tags

Venus

Expression

Mutation

Promoter

T7

Available since

Oct. 31, 2012

Availability

Academic Institutions and Nonprofits only

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pNIC28-Bsa4-ICL1

Plasmid

#105822

Purpose

Expression of Mycobacterium tuberculosis icl1 recombinant protein

Depositor

Ivanhoe Leung

Article

Bhusal et al Medchemcomm. 2017 Oct 17;8(11):2155-2163

Insert

icl1

Use

Tags

His tag with TEV cleavage

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pET-tTALE-EmGFP-15b

Plasmid

#138280

Purpose

Express tTALE-EmGFP

Depositor

Kyubong Jo

Article

Shin et al Sci Rep. 2019 Nov 20;9(1):17197. doi: 10

Insert

7-mer tTALE-EmGFP

Use

TALEN

Tags

6x His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

April 1, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p4973 pcDNA4-E6AP III C21Y

Plasmid

#32890

Depositor

Peter Howley

Article

Cooper et al J Biol Chem. 2004 Sep 24;279(39):41208-1

Insert

E6AP (UBE3A Human)

Use

Tags

6 His Express and T7-tag

Expression

Mammalian

Mutation

C21Y

Promoter

CMV

Available since

May 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL2402

Plasmid

#36805

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

ATM-TALEN_Left (Atm Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pET11-His6-Pif6-sfGFP-ssrA

Plasmid

#140891

Purpose

Expression of PIF-sfGFP-ssrA in E. coli

Depositor

Neal Devaraj

Article

Bhattacharya et al Proc Natl Acad Sci U S A. 2020 Aug 4;117

Insert

PIF-sfGFP-ssrA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTAMAHISTEV_BauA

Plasmid

#128952

Purpose

expresses BauA in E.coli

Depositor

James Naismith

Article

Moynie et al Elife. 2018 Dec 18;7. pii: 42270. doi: 1

Insert

ferric acinetobactin receptor

Use

Tags

TEV protease cleavable 7xHis and TamA

Expression

Bacterial

Mutation

Signal peptide sequence has been removed (1-66)

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pET-2HMG-mCherry

Plasmid

#112739

Purpose

Express 2HMG-mCherry in E. coli

Depositor

Kyubong Jo

Article

Lee et al RSC Adv, 2016, 6, 46291-46298

Insert

2HMG-mCherry

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXC13(human)-pET

Plasmid

#8679

Depositor

Corey Largman

Article

Komuves et al Dev Dyn 2003 Jun;227(2):192-202.

Insert

HOXC13 (HOXC13 Human)

Use

Tags

His and T7

Expression

Bacterial

Mutation

makes protein by Coomassie blue staining

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCellFree_G06_ZIKVC

Plasmid

#145349

Purpose

Cell-free expression vector for Zika virus Capsid protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV Capsid

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBH31(gfcD)

Plasmid

#176199

Purpose

Encodes pelC signal sequence followed by His6-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

pelC leader-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pBH31(gfcD-10xHis)

Plasmid

#176200

Purpose

Encodes pelC signal sequence followed by His10-GfcD. Should secrete to periplasm

Depositor

Mark Saper

Article

Larson et al PLoS One. 2021 Nov 15;16(11):e0259900. d

Insert

gfcD

Use

Tags

pelC leader-10xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 12, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pET29b_Qcat18

Plasmid

#45146

Depositor

Christopher Petzold

Article

Batth et al Metab Eng. 2014 Sep 6. pii: S1096-7176(1

Insert

E-coli-pathways-QconCAT18

Use

Tags

6XHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 5, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pKM263-EcPPXc

Plasmid

#38328

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

E. coli polyphosphatase (ppx Escherichia coli)

Use

Tags

6xHIS, GST, and TEV

Expression

Bacterial

Mutation

C-terminus of E. coli polyphosphatase (described …

Promoter

T7

Available since

Aug. 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 ANXA1

Plasmid

#67071

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ANXA1 (ANXA1 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
1BR9-mCherrySTOP

Plasmid

#193866

Purpose

E. coli expression vector for N-ter GFP11 tagged mCherry.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

mCherry

Use

Tags

6xHis and GFP11

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2274

Plasmid

#36711

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

MDM2-TALEN_Left (Mdm2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pET-28a-His6-SUMO-VAMP2

Plasmid

#135551

Purpose

Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

Depositor

Jingshi Shen

Article

Yu et al Methods Mol Biol. 2019;1860:237-249. doi

Insert

VAMP2

Use

Tags

6xHis tag, thrombin recognition and cleavage site…

Expression

Bacterial

Mutation

No ATG start codon

Promoter

T7 promoter

Available since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
mCherry-E2

Plasmid

#175242

Purpose

Bacterial expression and purification, E2 enzyme (UBC9) for SUMOylation

Depositor

Michael Rosen

Article

Peeples et al Nat Chem Biol. 2021 Jun;17(6):693-702. d

Insert

UBE2I (UBE2I Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
CENP-B mCherry PP1 gamma

Plasmid

#45221

Depositor

Michael Lampson, Iain Cheeseman

Article

Liu et al J Cell Biol. 2010 Mar 22;188(6):809-20.

Insert

CENP-B mCherry PP1 gamma

Use

Tags

Expression

Mammalian

Mutation

Promoter

T7

Available since

May 31, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pJK357

Plasmid

#71568

Purpose

Produces Acetobacter aceti 1023 succinyl-CoA:acetate CoA-transferase (AarC)

Depositor

T. J. Kappock

Article

Mullins et al J Bacteriol. 2008 Jul;190(14):4933-40. d

Insert

succinyl-CoA:acetate CoA-transferase

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 19, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT138b-proA

Plasmid

#138514

Purpose

P3g

Depositor

David Liu

Article

Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

Insert

T7-RNAP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

March 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pETM41-EcLonA

Plasmid

#38330

Depositor

Florian Freimoser

Article

Werner et al Fungal Genet Biol. 2007 Sep;44(9):845-52

Insert

Lon protease fragment (lon Escherichia coli)

Use

Tags

His, MBP, and TEV

Expression

Bacterial

Mutation

Polyphosphate binding fragment of Lon protease. …

Promoter

T7

Available since

Aug. 10, 2012

Availability

Academic Institutions and Nonprofits only
pET28a-Ec.coaBC (pCLK1210)

Plasmid

#50448

Purpose

Heterologous expression of E. coli CoA biosynthetic enzyme, PPCS_PPCDC - phosphopantothenoylcysteine synthetase_phosphopantothenoylcysteine decarboxylase

Depositor

Tadhg Begley, Erick Strauss

Article

Strauss et al J Biol Chem. 2001 Apr 27;276(17):13513-6

Insert

PPCS PPCDC

Use

Tags

6XHIS tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 6, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G06_ZIKV NS1

Plasmid

#145352

Purpose

Cell-free expression vector for Zika virus NS1 protein with C-term mCherry

Depositor

Rob Parton

Article

Varasteh Moradi et al J Proteome Res. 2020 Apr 3;19(4):1522-15

Insert

ZIKV NS1

Use

Tags

mCherry and 8xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellFree_G03 ISG15

Plasmid

#67054

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

ISG15 (ISG15 Human)

Use

Cell-free expression

Tags

EGFP and His

Expression

Mutation

Promoter

T7

Available since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pET14_GFP5_Encap

Plasmid

#86408

Purpose

Expresses sfGFP tagged with a 5 amino acid CLP and encapsulin as an operon

Depositor

David Savage

Article

Cassidy-Amstutz et al Biochemistry. 2016 Jun 21;55(24):3461-8.

Inserts

sfGFP with a 5aa CLP tag
Encapsulin

Use

Tags

6x Histidine

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21a - Mtb-TF

Plasmid

#111513

Purpose

Expresses M. tuberculosis Trigger Factor with a C-terminal His tag

Depositor

Judy Lieberman

Article

Dotiwala et al Cell. 2017 Nov 16;171(5):1125-1137.e11.

Insert

Trigger Factor

Use

Tags

His tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 25, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pNRB143

Plasmid

#36452

Depositor

Douglas Bishop

Article

Bishop et al Genes Cells. 1999 Aug;4(8):425-44.

Insert

RAD54 (RAD54 Budding Yeast)

Use

Tags

Expression

Yeast

Mutation

Promoter

T7

Available since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only
pET151-Vps4(101-437)

Plasmid

#87735

Purpose

Bacterial expression of N-terminally truncated S. cerevisiae Vps4

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV

Expression

Bacterial

Mutation

deleted amino acids 1-100

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
MSX1-pET

Plasmid

#21024

Depositor

Corey Largman

Article

Stelnicki et al Differentiation. 1997 Oct . 62(1):33-41.

Insert

MSX1 (Msx1 Mouse)

Use

Tags

His and T7 tag

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
TAL2344

Plasmid

#36764

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTEN-TALEN_Left (Pten Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2345

Plasmid

#36765

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

PTEN-TALEN_Right (Pten Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pET-30a(+)-Syk-tSH2

Plasmid

#111269

Purpose

express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)

Depositor

Carol Post

Article

Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5

Insert

murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
NdeI – SpeI(NheI) – BamHI/BglII - ANXA2 (23-339)

Plasmid

#136546

Purpose

Expresses ANXA2 chimeras in bacterial cells

Depositor

Laszlo Nyitray

Article

Ecsedi et al Structure. 2020 Aug 4;28(8):943-953.e4.

Type

Empty backbone

Use

Tags

ANXA2 (23-339) and His-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2362

Plasmid

#36775

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

EZH2-TALEN_Left (Ezh2 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

Showing: 5881 - 5920 of 10227 results

Results per page:

Sort results by: