We narrowed to 15,575 results for: nol
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MS2-epegRNA-Dnmt1
Plasmid#211372PurposeDnmt1 targeting epegRNA with MS2 insertion in gRNA scaffold for v3 PE-eVLP productionDepositorInsertMS2-epegRNA
ExpressionMammalianAvailable SinceJan. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
FokI-dCas9
Plasmid#52970PurposeExpresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cellsDepositorInsertFok1 fused to dCas9
UseCRISPRTagsFok1 nuclease (homodimeric wildtype) and NLSExpressionMammalianMutationD10A and H840A in Cas9PromoterCMVAvailable SinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDAC439
Plasmid#195237PurposeMammalian expression of Csm complex from single promoter; RFP backbone (Used for RNA knockdown)DepositorInsertFLAG-NLS-Csm5-2A-FLAG-NLS-Csm4-2A-FLAG-NLS-Csm3-2A-FLAG-NLS-Csm2; RFP-2A-FLAG-NLS-Cas6-2A-FLAG-NLS-Csm1; crRNA
ExpressionMammalianAvailable SinceJan. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-PspCas13b-gRNA-Actb1216
Plasmid#155368PurposePspCas13b guide RNA positioned 8bp 5' of Actb 1216 for targeted m6A RNA methylationDepositorInsertPspCas13b gRNA targeting Actb 1216
UseCRISPRExpressionMammalianAvailable SinceJuly 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLX-TRV2
Plasmid#180516PurposeAgrobacterium tumefaciens-based expression of TRV2 including a PEBV heterologous promoter and a polylinkerDepositorInsertTobacco rattle virus RNA2 with a PEBV heterologous promoter and a polylinker
ExpressionPlantPromoter35S CaMVAvailable SinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX_311-Cas9
Plasmid#96924Purposefor Cas9 knockout, lentiviral expression of Cas9. Alternate plasmid name: pXR111DepositorInsertCas9
UseLentiviralTags3xFlag and V5ExpressionMammalianPromoterEF1aAvailable SinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyExpressionBacterialPromoterPveg promoter with CuO sequence and PxylR promoterAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-epegRNA-SV40-puro
Plasmid#240539PurposeLentiviral backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.DepositorInsertepegRNA
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
enAsABE8e
Plasmid#138506PurposeA-to-G base editorDepositorInsertecTadA(8e)-dAsCas12a (En)
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-Trono-EFS-Blast-P2A-TurboRFP
Plasmid#228893PurposeCloning and expression of gRNAs or pegRNAs with EcoRI/Esp3I insertion.DepositorTypeEmpty backboneUseCRISPR and LentiviralExpressionMammalianAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-M13-TEV-C-P2A-TdTomato
Plasmid#92391Purposeproduces AAV expressing Cal-light M13-TevC component and TdTomatoDepositorInsertM13-TEV-C-P2A-TdTomato
UseAAV and Synthetic BiologyTagsTdTomatoExpressionMammalianPromoterhuman synapsinAvailable SinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
TtTMPV-Neo
Plasmid#27993PurposeRetroviral transfer plasmid for Tet-regulated shRNA expression with a TREtight promoter. Contains TurboRFP and Venus fluorescent reporters.DepositorInsertTtTMPV-Neo (shRen)
UseRNAi and Retroviral; Fluorescent reportersExpressionMammalianAvailable SinceApril 15, 2011AvailabilityAcademic Institutions and Nonprofits only -
TurboID-V5_pRS415
Plasmid#107167Purposeexpresses V5-tagged TurboID in the yeast cytosolDepositorInsertTurboID (BirA mutant)
ExpressionYeastMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
C1(1-29)-TurboID-V5_pLX304
Plasmid#107175Purposeexpresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vectorDepositorInsertTurboID (BirA mutant)
UseLentiviralMutationQ65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…Available SinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT277
Plasmid#122608Purposeexpress evoCDA1-BE4max in mammalian cellsDepositorInsertevoCDA1-BE4max
ExpressionMammalianMutationF23S A123V I195FAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a-GlyRS-His
Plasmid#124110PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceApril 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pComb3XLambda
Plasmid#63892PurposeBackbone pComb3X containing PCR template for human antibody lambda light chain constant regionDepositorInserthuman antibody lambda light chain (IGLL5 Human)
UsePhage displayExpressionBacterialPromoterlacZAvailable SinceJuly 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap entry vector
Plasmid#188249PurposeCloning vector for generating template plasmids to transcribe mRNA, with mNeonGreen dropout following BsaI digestionDepositorInsertmNeonGreen
ExpressionMammalianPromoterT7Available SinceSept. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHSB04X
Plasmid#117258PurposeGenome editing for Lactobacillus brevis ATCC367DepositorInsertCas9, promotor PslpA-guide-RNA, homologous arms of Lb_1019
UseOtherAvailable SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pQE30-His-PheRS
Plasmid#124116PurposeBacterial expression plasmid for PURE system componentsDepositorAvailable SinceJune 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAB1619 hU6-BpiI-Cas13bt1-DR
Plasmid#176314PurposehU6-BpiI-Cas13bt1-DR (crRNA expression)DepositorInsertCas13bt1 crRNA direct repeat
UseCRISPRExpressionMammalianAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Puro Xlone-eGFP
Plasmid#136936Purposedonor plasmid for inducible transgene expression in human cellsDepositorInsertall-in-one tet-on system
UseSynthetic BiologyExpressionMammalianPromoterTRE3GSAvailable SinceApril 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330 spCas9-mSA
Plasmid#113096PurposepX330 vector carrying spCas9-mSA for gene editing in mammalian cell linesDepositorInsertCAS9-MSA
UseSynthetic BiologyTagsMSA (monomeric streptavidinMutationnoneAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas9 TLS2
Plasmid#196978PurposeExpresses Cas9 fused with TLS2 mobility signal that confers graft mobility. Induced by estradiol.DepositorInsertCas9 with TLS2 tag
ExpressionPlantPromoterpG10-90Available SinceMay 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
LbCas12a-crRNA
Plasmid#213753PurposeFor circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cellsDepositorInsertLbCas12a-crRNA
UseCRISPRExpressionMammalianPromoterU6Available SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBMNb-cALFA
Plasmid#159991PurposeFor the protein expression with ALFA tag at C-terminus in mammalian cellsDepositorTypeEmpty backboneTagsHRV3C protease cleavage site-ALFA tag-twin Strep …ExpressionMammalianMutationCMV promoter, WPREAvailable SinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
BPK3082
Plasmid#78742PurposeHuman expression plasmid for LbCpf1 guide RNA (need to clone in spacer into BsmBI sites)DepositorInsertLbCpf1 crRNA backbone, without spacer sequence
UseCRISPRExpressionMammalianPromoterU6Available SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
SaABE8e
Plasmid#138500PurposeA-to-G base editorDepositorInsertecTadA(8e)-nSaCas9
ExpressionMammalianAvailable SinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pP3-GFP
Plasmid#53436PurposeControls GFP expression from the P3 promoter cloned out of S. aureus RN4220 genomeDepositorInsertP3 promoter
UseSynthetic BiologyTagsnoneExpressionBacterialMutationnonePromoterP3Available SinceJune 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
nLbCas12a
Plasmid#213745PurposeFor circular RNA-mediated prime editor using nLbCas12a-D156R-R1138A in HEK293T cellsDepositorInsertnLbCas12a
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, R1138APromoterCMVAvailable SinceFeb. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
SB_concave
Plasmid#110102PurposepBXPHM3 with scaffold Sybody of the concave libraryDepositorInsertscaffold Sybody of the concave library
TagsHisExpressionBacterialPromoterPBADAvailable SinceMay 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
QPM-sgR (pTaU3)
Plasmid#140448PurposeFor sgRNA expression in monocotyledons protoplastsDepositorInsertTaU3-sgRNA
UseCRISPRExpressionPlantPromoterTaU3Available SinceMay 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL01009::AtU6p
Plasmid#46968PurposeEncodes the Arabidopsis U6 promoter in a level 0 vector (Weber et al. PLoS One 6:e16765, 2011). It is used to place an sgRNA uder the Arabidopsis U6 promoter.DepositorInsertAtU6p
UseCRISPRExpressionPlantAvailable SinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
SpRY-Tad-CBE(ACC3)
Plasmid#235082PurposeExpresses SpRY-Tad-CBE(ACC3) in mammalian cells.DepositorInsertTad-CBE(ACC3)-SpRY Cas9 D10A
UseCRISPRTags2xUGI, bpNLS and bpNLSExpressionMammalianAvailable SinceJuly 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable SinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTK312
Plasmid#59569PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP XbaI attB1 Kozak EGFP ochre attB2 AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP XbaI attB1 Kozak EGFP ochre attB2 AscI
Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMK407 (BSD-P2A-mAID-Nluc)
Plasmid#121197PurposeN-terminal taggingDepositorInsertBSD-P2A-mAID-Nluc
UseCRISPRAvailable SinceFeb. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLenti-U6-epegRNA eBAR1-SV40-puro
Plasmid#240535PurposeLentiviral eBAR1 backbone plasmid to insert epegRNAs (tevopreq1) of interest with puromycin resistance. The cloning site is BsmBI.DepositorInsertepegRNA-eBAR1
UseCRISPR and LentiviralExpressionMammalianAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST-hisMBP-AsCpf1-EC
Plasmid#79007PurposeExpressing his-MBP tagged AsCpf1 (E.coli codon optimized)DepositorInsertAsCpf1
Tags6xHis-MBP-NLS and NLS-HAExpressionBacterialPromotertac promoterAvailable SinceJuly 22, 2016AvailabilityAcademic Institutions and Nonprofits only
