We narrowed to 7,782 results for: val
-
Plasmid#83557PurposeUsed to evaluate the expression output of GAL10 promoter with yEGFP used as the reporter gene.DepositorInsertGAL10 promoter-yEGFP
ExpressionYeastPromoterGAL10Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-1
Plasmid#63826PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-2
Plasmid#63827PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-3
Plasmid#63828PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-4
Plasmid#63829PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-5
Plasmid#63830PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-6
Plasmid#63831PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-7
Plasmid#63832PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-8
Plasmid#63833PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-9
Plasmid#63834PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTd-4-15-13-11
Plasmid#63836PurposeThis plasmid is designed to validate the biophysical model of translational couplingDepositorInsertCFP, mRFP1 and GFPmut3b
ExpressionBacterialMutationThe intergenic distance between each gene is -4 n…PromoterJ23100Available SinceFeb. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK130
Plasmid#71129PurposeExpression of Acetobacter aceti 1023 N5-carboxyaminoimidazole ribonucleotide synthetase (PurK) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide synthetase
TagsHis6ExpressionBacterialMutationchanged valine-319 to phenylalaninePromoterT7Available SinceFeb. 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK314
Plasmid#71140PurposeExpression of Acetobacter aceti 1023 PurE (H89V mutant) in E. coliDepositorInsertN5-carboxyaminoimidazole ribonucleotide mutase
ExpressionBacterialMutationchanges histidine-89 to valine; additional N-term…PromoterT7Available SinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Spec(x2)-DocI
Plasmid#58713PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertSpec(x2)-DocI
TagsHIS, HRV 3C, and ybbRExpressionBacterialPromoterT7Available SinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAC-EsaR-D91G/V220A
Plasmid#47648PurposepAC-EsaR with a272g and c659t mutations in esaRDepositorInsertesaR-D91G/V220A
UseSynthetic BiologyExpressionBacterialMutationChanged Aspartic acid 91 to Glycine, and Valine 2…PromoterPlacAvailable SinceSept. 6, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B1-DK
Plasmid#117981PurposeSdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B1
TagsHIS, sortase, and ybbrExpressionBacterialPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B2-DK
Plasmid#117982PurposeSdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
TagsHIS, sortase, and ybbrExpressionBacterialPromoterT7AvailabilityAcademic Institutions and Nonprofits only -
pSF2C-A-RFP
Plasmid#172432PurposeStrepFlex MoClo vector for E.coli cloning and Streptomyces chromosomal integration - equivalent of pTU2-A-RFP from EcoFlex (ACS Synth. Biol. 2016, 5, 10, 1059–1069)DepositorTypeEmpty backboneUseSynthetic BiologyTagsNoneExpressionBacterialAvailable SinceAug. 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAEMXT-eLACCO1.1
Plasmid#167946PurposeFluorescent biosensor for extracellular L-lactateDepositorInsertCD59-eLACCO1.1-GPI
ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGE-attBTT-loxP-DsRed (pTL780)
Plasmid#115160PurposeIntegration vector for phiC31-mediated transgenesis using a genomic standard attP-TT site. Contains an attB-TT site, the visible 3xP3-DsRed marker flanked by a loxP site for Cre-mediated removal.DepositorInsertattB-TT loxP 3xP3-DsRed
UseCre/LoxExpressionInsectAvailable SinceOct. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAEMXT-deLACCO
Plasmid#167947PurposeFluorescent biosensor control for extracellular L-lactateDepositorInsertCD59-deLACCO-GPI
ExpressionMammalianPromoterCMVAvailable SinceApril 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-SnoopCatcher
Plasmid#72322PurposeBacterial expression of SnoopCatcher, an engineered protein forming a spontaneous covalent bond with its cognate peptide partner SnoopTag.DepositorInsertpET28a-SnoopCatcher
TagsHis6 tagExpressionBacterialMutationchanged G842T and D848G of S. pneumoniae adhesin …PromoterT7Available SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET28a SnoopTag-MBP
Plasmid#72323PurposeBacterial expression of Maltose Binding Protein linked to SnoopTag, a peptide tag forming a spontaneous covalent bond with its protein partner Snoopcatcher.DepositorInsertpET28a SnoopTag-MBP
TagsN-terminal His6 tag. MBP is fused at the C-termin…ExpressionBacterialAvailable SinceFeb. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-LbNOX-FLAG
Plasmid#218631PurposeThis plasmid contains LbNOX under doxycycline inducible control.DepositorInsertLbNOX
UseLentiviral and Synthetic BiologyTagsFLAGExpressionMammalianAvailable SinceJuly 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
murine TCR OTI-2A.pMIG II
Plasmid#52111Purposeretroviral expression of both TCRalpha and TCRbeta using a single vectorDepositorUseRetroviralTagsT2A (from Thosea asigna virus)ExpressionMammalianAvailable SinceApril 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-VSVG
Plasmid#11912DepositorInsertVSVG
ExpressionMammalianMutationF64L, S65T, A206K relative the wild type GFP sequ…Available SinceSept. 13, 2006AvailabilityAcademic Institutions and Nonprofits only -
pLVX-TetOne-Puro-mitoLbNOX-FLAG
Plasmid#218667PurposepLVX-TetOne-Puro-mitoLbNOX-FLAGDepositorInsertmitoLbNOX
UseLentiviral and Synthetic BiologyTagsFLAG and MTSExpressionMammalianAvailable SinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBbA5c-MevT(CO)-MBIS(CO, ispA)
Plasmid#35151DepositorInsertAtoB(co)-HMGS(co)-HMGR(co)-MK(co)-PMK(co)-PMD-Idi-IspA
UseSynthetic BiologyExpressionBacterialMutationR364S in Mevalonate KinasePromoterLacUV5,Available SinceJuly 23, 2012AvailabilityAcademic Institutions and Nonprofits only -
pgRNA-non-target
Plasmid#129465PurposeDerived from pSCB2-sgRNA. 20 nucleotide sequence added as sgRNA binding region by inverse PCR. Used as a negative control.DepositorInsertNon-target/random gRNA
UseCRISPRExpressionBacterialPromoterBBa_J23119 (SpeI)Available SinceSept. 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCDH-CMV-mC-G-LC3B-P
Plasmid#124974Purposeevaluate autophagyDepositorInsertmCherry-GFP-LC3B
UseLentiviralPromoterCMVAvailable SinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-T2A-moxMaple3
Plasmid#120875PurposeMammalian expression of mCherry and moxMaple3DepositorInsertsmCherry
moxMaple3
ExpressionMammalianMutationfrom mMaple3, moxMaple3 was engineered by replaci…Available SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI SMN1
Plasmid#72286PurposeSMN1 exon 7 splicing cassetteDepositorAvailable SinceJan. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSSA-DsRed/eGFP (pXK-442)
Plasmid#208827PurposeThe SSA-based surrogate reporter for validating the activity of different gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CMV-DsRed-CMV-eGFP*(SSA).DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDule-tfmF A65V S158A
Plasmid#85484PurposeMachinery plasmid expressing permissive Mj tfmF synthetase and cognate amber suppressing tRNADepositorInserttri-fluoromethyl-phenylalanine Mj synthetase A65V S158A
TagsNoneExpressionBacterialMutationY32V F108W Q109M I159M with 65Val and 158AlaPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBA-mCherry-EGFP-PIM
Plasmid#111758PurposeLow level expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
ExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …Promoterchicken beta-actinAvailable SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
YFP-Cdc42(V12)
Plasmid#11399DepositorInsertcell division cycle 42 (CDC42 Human)
TagsYFPExpressionMammalianMutationObtained with Glycine 12 changed to Valine.Available SinceFeb. 21, 2007AvailabilityAcademic Institutions and Nonprofits only -
pUAS-V5-miniTurboID-NES
Plasmid#116905PurposeExpresses miniTurboID under Gal4/UAS control. miniTurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorInsertminiTurboID
UseDrosophila expressionAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAS-V5-TurboID-NES
Plasmid#116904PurposeExpresses TurboID under Gal4/UAS control. TurboID is tagged at the N-terminus with V5 and a nuclear export signal (NES) at the C-terminus.DepositorInsertTurboID
UseDrosophila expressionAvailable SinceOct. 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGW1-mCherry-EGFP-PIM
Plasmid#111759PurposeHigh expression of mCherry-EGFP-PIM. Can be used for inducible aggregate formation upon AP20187 additionDepositorInsert2x FKBP homo-mCherry-EGFP-2x FKBP homo-2x FKBP
ExpressionMammalianMutationVal24Glu, Tyr80Cys, Ala95Thr mutations in second …PromoterCMVAvailable SinceOct. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
14x PP7 with loxP-kanMX-loxP
Plasmid#189939PurposeIntegration of 14xPP7 loops in Saccharomyces cerevisiae. Selection by KanMX (flanked by LoxP sites for marker removal)DepositorInsert14x PP7 with loxP-kanMX-loxP
TagskanMXExpressionBacterialAvailable SinceFeb. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
GFP-Ub KO.G76V
Plasmid#11932DepositorInsertUbiquitin KO.G76V (RBX1 Human)
TagsGFPExpressionMammalianMutationConjugation deficient mutant. All 7 lysines of ub…Available SinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSSA-eGFP (pXK-221)
Plasmid#208826PurposeThe SSA-based surrogate reporter for validating the activity of gene editing tools as well as for helping to screen gene-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(SSA).DepositorTypeEmpty backboneExpressionMammalianPromoterCMVAvailable SinceDec. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pILGFPI7
Plasmid#83555PurposeUsed to evaluate the expression output of CUP1 promoter with yEGFP used as the reporter gene.DepositorInsertCUP1 promoter-yEGFP
ExpressionYeastPromoterCUP1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPB5A
Plasmid#83556PurposeUsed to evaluate the expression output of GAL1 promoter with yEGFP used as the reporter gene.DepositorInsertGAL1 promoter-yEGFP
ExpressionYeastPromoterGAL1Available SinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP91
Plasmid#83548PurposeUsed to evaluate the expression output of PGK1 promoter with yEGFP used as the reporter gene.DepositorInsertPGK1 promoter-yEGFP
ExpressionYeastPromoterPGK1Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19_dTAG-KANSL3-targeting
Plasmid#170755PurposeDonor plasmid for BSD-P2A-2xHA-dTAG knock-in into the N-terminus of the mouse Kansl3 locusDepositorInsertBSD-P2A-2xHA-dTAG
UseMouse TargetingTagsBSD-P2A-2xHA-dTAGMutationPhenylalanine 36 to valine in FKBP12Available SinceJune 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Zebrabow-GateDest
Plasmid#118221PurposeFor cloning promoter into a Zebrabow vector with Gateway reaction. Only compatible with single site Gateway. Not compatible with Tol2-kit.DepositorInsertBrainbow 1.0 L - ccdB survival cassette
UseZebrafish expressionAvailable SinceNov. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-fPV-GFP
Plasmid#22914DepositorInsertfugu parvalbumin promoter driving GFP
UseAAVExpressionMammalianAvailable SinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH3
Plasmid#83553PurposeUsed to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.DepositorInsertHXT7 promoter-yEGFP
ExpressionYeastPromoterHXT7Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP92
Plasmid#83549PurposeUsed to evaluate the expression output of TDH3 promoter with yEGFP used as the reporter gene.DepositorInsertTDH3 promoter-yEGFP
ExpressionYeastPromoterTDH3Available SinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only