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pRSAN-QUE37C

Plasmid

#129312

Purpose

ATP measurement

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-37C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.JARID2

Plasmid

#125166

Purpose

Expresses human JARID2 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

JARID2 (JARID2 Human)

Use

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRSET-B YFP

Plasmid

#108856

Purpose

Encodes for the fluorescent protein enhanced YFP (eYFP)

Depositor

Kalina Hristova

Article

Sarabipour et al Anal Biochem. 2014 Mar 15;449:155-7. doi

Insert

eYFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII

Plasmid

#45064

Purpose

Expression of CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

RSV

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-gst-xr

Plasmid

#214761

Purpose

Xylose reductase from Hypocrea jecorina fused with GST-tagged and codon optimized for expression in E. coli

Depositor

Pimchai Chaiyen

Article

Jaroensuk et al J Biol Chem. 2023 Dec 28:105598. doi: 10

Insert

glutathione S-transferases fused with xylose reductase

Use

Tags

Glutathione S-transferases

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRSV-p65T305A

Plasmid

#106454

Purpose

NF-kB p65, kinase site 305 mutated

Depositor

Georg Weber

Article

He et al Eur J Biochem. 2003 May . 270(10):2174-8

Insert

NF-kB p65, kinase site 305 mutated (RELA Human)

Use

Tags

Expression

Mammalian

Mutation

kinase site inactivated

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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vgCam-pRSETb

Plasmid

#193362

Purpose

A FRET-based calcium probe using Sumire

Depositor

Takeharu Nagai

Article

Sugiura et al Commun Biol. 2022 Nov 3;5(1):1172. doi:

Insert

vgCam

Use

E.coli expression

Tags

His X6 tag and Strep tag

Expression

Mutation

Promoter

T7

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pFB1.HMBP.PrS.SUZ12

Plasmid

#125162

Purpose

Expresses human SUZ12 in insect cells, , under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Zhang et al Nat Struct Mol Biol. 2019 Mar;26(3):237-

Insert

Polycomb protein SUZ12 (SUZ12 Human)

Use

Baculovirus expression vector

Tags

His-MBP

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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pRSF-T20S

Plasmid

#110805

Purpose

expresses Thermoplasma acidophilium 20S proteasome in E. Coli

Depositor

Yifan Cheng

Article

Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

Inserts

PsmB
PsmA

Use

Tags

tev-His

Expression

Bacterial

Mutation

N2D and Q2E

Promoter

T7 and T7 promoter

Available since

July 6, 2018

Availability

Academic Institutions and Nonprofits only

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pRS316-4M5.3

Plasmid

#45831

Depositor

Dane Wittrup

Insert

4M5.3

Use

Tags

FLAG

Expression

Yeast

Mutation

Promoter

GAL1

Available since

June 26, 2013

Availability

Academic Institutions and Nonprofits only

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pRSETb-ffDronpa

Plasmid

#78182

Purpose

Expresses ffDronpa E. coli strains

Depositor

Peter Dedecker

Article

Moeyaert et al ACS Nano. 2014 Feb 25;8(2):1664-73. doi:

Insert

ffDronpa

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRSF-1b-S14

Plasmid

#128596

Purpose

Expresses tagless 30S ribosomal subunit protein S14 in E.coli

Depositor

James Williamson

Article

Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

Insert

30S ribosomal subunit protein S14 (rpsN E.coli)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 25, 2022

Availability

Academic Institutions and Nonprofits only

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pRSAN-QUE20C

Plasmid

#129316

Purpose

QUEEN ATP sensor for 20degC

Depositor

Yasushi Okada

Article

Yaginuma et al bioRxiv 2021.10.08.463131

Insert

QUEEN-20C

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-TMPRSS2

Plasmid

#158441

Purpose

Gateway Cloning compatible entry vector for the human TMPRSS2 gene.

Depositor

Alejandro Chavez, Sho Iketani

Insert

TMPRSS2 (TMPRSS2 Human)

Use

Gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pRS314-URA3

Plasmid

#11004

Depositor

Francisco Malagon

Insert

URA3 (URA3 Budding Yeast)

Use

Tags

Expression

Bacterial and Yeast

Mutation

Wild type

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only

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pRSETb-rsGreen0.7

Plasmid

#78176

Purpose

Expresses rsGreen0.7 in E. coli strains.

Depositor

Peter Dedecker

Article

Duwe et al ACS Nano. 2015 Oct 27;9(10):9528-41. doi

Insert

rsGreen0.7

Use

Tags

6xHis-tag; Xpress tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_DARPinOFF7

Plasmid

#182396

Purpose

Bacterial expression of DARPinOFF7

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

DARPinOFF7

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pRSETB-de4GFP

Plasmid

#85472

Purpose

Inducible expression of dual emitting pH sensitive GFP in bacterial cells

Depositor

S. James Remington

Article

Hanson et al Biochemistry. 2002 Dec 31;41(52):15477-8

Insert

de4GFP

Use

Tags

6xHis

Expression

Bacterial

Mutation

S65T/C48S/H148C/T203C

Promoter

T7

Available since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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Lapronic/pRSETB

Plasmid

#140755

Purpose

FRET-Based Genetically Encoded Nanosensor for Lactate/Pyruvate Ratio

Depositor

Luis Felipe Barros

Article

Galaz et al Anal Chem. 2020 Jun 29. doi: 10.1021/acs

Insert

Lapronic (Lactate/PYRuvate Ratio Optical Nano-Indicator from CECs)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pRset-OppA

Plasmid

#207869

Purpose

Bacterial expression of poly-his-tagged L. lactic OppA

Depositor

Mathew Tantama

Article

Norcross et al ACS Omega. 2019 Feb 28;4(2):2812-2822. d

Insert

OppA

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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