We narrowed to 4,603 results for: Abo
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Plasmid#92309PurposeKnock eGFP into human VE-cad locusDepositorInsertVEcad Homologous Recombination Arms (CDH5 Human)
UseCRISPRExpressionMammalianMutationabout 30 a.a. truncated at end of proteinAvailable SinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pU6-ACTA2_gRNA1-SpCas9-T2A-GFP
Plasmid#126706Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human ACTA2 locus at the end of the endogenous coding sequenceDepositorInsertACTA2_gRNA1-SpCas9-T2A-GF (ACTA2 Human)
UseCRISPRAvailable SinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pX330-sgR26
Plasmid#127376PurposeUsed for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.DepositorInsertROSA26 (Gt(ROSA)26Sor Mouse)
UseCRISPR and Mouse TargetingAvailable SinceFeb. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pINDUCER dCas9-TET1CDMut
Plasmid#101920PurposeThe dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.DepositorInsertdCas9-TET1CD Mut
UseLentiviralExpressionMammalianMutationMutated TET1 catalytic domainAvailable SinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF1a-RoninDHSA-FLAG-IRES-Neo
Plasmid#28021DepositorInsertRonin (THAP11 Human)
TagsFLAGExpressionMammalianMutationDHSA mutation (Y246A) of the previously defined H…Available SinceMay 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2
Plasmid#86237PurposeLentivirus for expression of human Plexin-B2DepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralExpressionMammalianMutationfull length PLXNB2, CRISPR resistantPromoterhuman PGK promoterAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFB-U6-sgNeuN-gRNA-hSyn-mCherry-WPRE-SV40pA
Plasmid#128346PurposepAAV encoding control gRNA for CRISPR gRNAs listed aboveDepositorAvailable SinceJuly 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
Str-STIM1-NN_puromycin
Plasmid#65310PurposeLuminal ER hook only (RUSH system)DepositorInsertSTIM1 mutated for binding motif to microtubule (STIM1 Human)
TagsstreptavidinExpressionMammalianMutation2 amino acids of STIM1 (IP from SxIP motif) were …PromoterCMVAvailable SinceJune 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCI-MS2V5-eRF3a F76a
Plasmid#65811Purposeexpression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1DepositorInserteRF3a (GSPT1 Human)
TagsMS2 stem loops and V5ExpressionMammalianMutationF76aPromoterCMVAvailable SinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-H2BmGreenLantern
Plasmid#177332PurposeTo express a bright monomeric green FP to label eucaryotic cell nuclei. To be used in clearing and other fluorescent microscope methods.DepositorInsertH2B (Hist1h2bq Rat)
UseAAVTagsmGreenLanternExpressionMammalianMutationThis fusion protein was optimized to the Human co…PromoterCAGAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro1 E208K/E328K
Plasmid#47894PurposeExpresses myc tagged Miro1 E208K/E328K mutantDepositorInsertMiro1 E208K/E328K (RHOT1 Human)
TagsmycExpressionMammalianMutationE208K/E328K, abolishes calcium bindingPromoterCMVAvailable SinceSept. 18, 2013AvailabilityAcademic Institutions and Nonprofits only -
GRM6-DuET
Plasmid#213304PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable SinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pRK5-myc-Miro2 E208K/E328K
Plasmid#47900PurposeExpresses myc tagged Miro2 V208K/E328K mutantDepositorInsertMiro2 E208K/E328K (RHOT2 Human)
TagsmycExpressionMammalianMutationE208K/E328K, abolishes calcium bindingPromoterCMVAvailable SinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCCLc-MND-B2705-KK10-SABR
Plasmid#119053Purpose3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBIDepositorInsertB2705-KK10-SABR
UseLentiviralExpressionMammalianPromoterMNDAvailable SinceJan. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV2-CAGIG-H2B-V5-mScarlet
Plasmid#191093PurposeTo express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methodsDepositorInsertH2B and mScarlet
UseAAVTagsH2B-V5-mScarletExpressionMammalianMutationThe fusion protein was optimized to the Human cod…PromoterCMV and CAGAvailable SinceDec. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGenLenti V5-BNIP3
Plasmid#223732PurposeStable expression of protein in mammalian cell cultureDepositorAvailable SinceMarch 25, 2025AvailabilityAcademic Institutions and Nonprofits only -
SOX17-NLS-tdTomato-EPG
Plasmid#210466PurposeDonor plasmid for knock-in NLS-tdTomato into the human SOX17 locusDepositorInsertSOX17 homologous recombination arms with a NLS-tdTomato and EF1alpha-drived puromycin-EGFP selection cassette (SOX17 Human)
UseCRISPRAvailable SinceJan. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUT_mNF-Cas13d-Triplex
Plasmid#227709PurposeLPUtopia matching RMCE donor plasmid with mCherry-P2A-RfxCas13d Negative-Feedback circuit. Include a 3' UTR Triplex motif as a template backbone for MONARCH 2.0 system.DepositorInsertmCherry-P2A-RfxCas13d-Triplex
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterCMV-d2iAvailable SinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pU6-SCGB3A2_gRNA1-SpCas9-T2A-GFP
Plasmid#126698Purposeto express Cas9 from S. pyogenes with 2A-EGFP and sgRNA targeting human SCGB3A2 locus at the end of the endogenous coding sequenceDepositorInsertsgRNA targeting human SCGB3A2 locus
ExpressionMammalianPromoterHuman U6Available SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAF197
Plasmid#135930PurposeAll-in-one Sleeping Beauty vector for Expression for Tasmanian devil CTLA4-Fc-mCherry fusion protein. Multiple cloning sites to swap genes-of-interest (i.e. CTLA4).DepositorInsertCTLA4 fused to IgG Fc and to mCherry
UseTransposonTagsmCherryExpressionMammalianPromoterEF1aAvailable SinceJan. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2-mGAP
Plasmid#86241PurposeLentivirus for expression of human Plexin-B2 with mutant GAP domainDepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralExpressionMammalianMutationPLXNB2 with point mutations of GAP domainPromoterhPGKAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TGt
Plasmid#44508DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hGRM1-RFP
Plasmid#22920DepositorInserthuman receptor metabotropic 1 promoter driving DsRedExpress (GRM1 Human)
UseAAVExpressionMammalianAvailable SinceMay 25, 2010AvailabilityAcademic Institutions and Nonprofits only -
pDN-T1TCbt
Plasmid#44514DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
HP1-ECFP-SNAP
Plasmid#187966Purposeexpresses HP1 with C-terminal tags ECFP and SNAPDepositorAvailable SinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDN-T1GZmbh
Plasmid#44522DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationThree tandem tet operators (3XtetO2) downstream o…PromoterPGal1-T123 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1TCbt
Plasmid#44515DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 LIC 6A NLRP1-FLAG E1322R
Plasmid#166809PurposeMammalian cell expression vector for FLAG-tagged NLRP1. The E1322R mutant abolishes interface III UPA-UPA contacts, DPP9 binding, and inflammasome activityDepositorAvailable SinceMarch 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDN-G1GZmbh
Plasmid#44516DepositorInsertsUseSynthetic Biology; Expression regulator/reporterExpressionYeastMutationTwo tandem tet operators (2XtetO2) downstream of …PromoterPGal1-D12 promoterAvailable SinceApril 23, 2013AvailabilityAcademic Institutions and Nonprofits only -
pLV-PLXNB2-decto
Plasmid#86238PurposeLentivirus for expression of human Plexin-B2 with deletion of ectodomainDepositorInsertPlexin-B2 (PLXNB2 Human)
UseLentiviralExpressionMammalianMutationPLXNB2 with deletion of extracellular domainPromoterhPGKAvailable SinceJan. 13, 2021AvailabilityAcademic Institutions and Nonprofits only