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Plasmid#141047PurposeMutisite Gateway mediated vector constructionDepositorInsertCsy4-2A-FokI-dCas9-2A-EGFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-TO-EGFP-UPF1-R615A
Plasmid#136001PurposeDOX-inducible R615A UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationR615APromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5 FRT-TO-EGFP-UPF1-DEAA
Plasmid#136002PurposeDOX-inducible DEAA UPF1 inserted with GFP tagged on the N-terminus and used with the Flp-In T-Rex SystemDepositorInsertUPF1
UseTagsEGFPExpressionMammalianMutationDE647AAPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 21-55 eGFP SV40
Plasmid#132653PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 20 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
Hy_pMtnA UTR delta 41-55 eGFP SV40
Plasmid#132652PurposeExpresses an eGFP mRNA from the Drosophila Metallothionein A promoter that has 40 nt of the MtnA 5' UTRDepositorInserteGFP
UseTagsExpressionInsectMutationPromoterMtnAAvailable sinceOct. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET51b(+)_EGFP-LAMA-S98 (for p24)
Plasmid#130717PurposePlasmid encoding the bacterial expression of LAMA-S98 for p24 HIV Capsid proteinDepositorInsertEGFP-LAMA-S98
UseTagsHisx10 and StrepTagExpressionBacterialMutationDeleted first Met, Ala and Last Ser from the Enha…PromoterAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
MRLC-DD-pIRES-EGFP-AP4-mito
Plasmid#101287PurposeTo define the role of Mena/VASP in tension-sensitive actin assemblyDepositorInsertMRLC-DD and AP4-Mito
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceMarch 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
psubCMV-WPRE-IL3SP-mVEGF-D-dNdC
Plasmid#119229PurposePlasmid encodes proteolytically cleaved murine VEGF-D deltaN deltaC form, which has signal peptide from murine IL3 gene to be used for stimulation of lymphangiogenesis in mice in vivo.DepositorInsertIL3SP-Vegfd-dNdC (Vegfd Mouse)
UseAAVTagsExpressionMutationPromoterCMVAvailable sinceMarch 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1079 - pAAV TH gRNA A EF1a EGFP
Plasmid#113159PurposeAn AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporterDepositorInsertgRNA for rat TH
UseAAV and CRISPRTagsExpressionMammalianMutationPromotermU6Available sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-puro-eGFP-HEC1-d80
Plasmid#114025PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorInserteGFP-d80-HEC1 (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 1 to 80 deletedPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d80
Plasmid#114030PurposeInducible expression of eGFP-tagged HEC1-d80 in FLPin Trex cell linesDepositorInserteGFP-d80-HEC1 (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 1 to 80 deletedPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-8A/S15D
Plasmid#114031PurposeInducible expression of eGFP-tagged HEC1-8A/S15D in FLPin Trex cell linesDepositorInserteGFP-HEC1-8A/S15D (NDC80) (NDC80 Human)
UseTagsExpressionMammalianMutationHec1 - 8A/S15DPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-HEC1-d207
Plasmid#114051PurposeExpression of exogenous eGFP HEC1 delta 207DepositorInserteGFP-HEC1-d207 (NDC80 Human)
UseTagsExpressionMammalianMutationHEC1 delta 207PromoterAvailable sinceSept. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-hygro-eGFP-STOP-HEC1
Plasmid#114026PurposeInducible expression of eGFP-tagged HEC1 in FLPin Trex cell lines. Due to insertion of a STOP-codon after the eGFP-tag, this plasmid only expressed eGFP.DepositorInserteGFP-STOP-HEC1 (NDC80 Human)
UseTagsExpressionMammalianMutationStart codon of Hec1 is mutated to stop codonPromoterAvailable sinceSept. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 R290A D291A T292A
Plasmid#112103PurposeMammalian expression of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationR290A D291A T292APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 T292A L293A P294A
Plasmid#112104PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationT292A L293A P294APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 L330R Q334R L702R
Plasmid#112105PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationL330R Q334R L702RPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406A M407A F409A
Plasmid#112108PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406A M407A F409APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R F409A
Plasmid#112110PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406R M407R F409APromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1 Dynamin1 D406R M407R T488W
Plasmid#112111PurposeMammalian expression plasmid of GFP-tagged Dynamin protein.DepositorInsertDynamin1 (DNM1 Human)
UseTagsEGFPExpressionMammalianMutationD406R M407R T488WPromoterCMVAvailable sinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP Kind2 S159/181/666E
Plasmid#105314PurposeTo study altered downstream functions of phospholylation-site mimetic kindlin2DepositorInsertKindlin2 (FERMT2 Human)
UseTagsEGFPExpressionMammalianMutationS159/181/666EPromoterAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-Akt2(K14A/R25E)-Halo-PS
Plasmid#86632PurposeExpresses eGFP-Akt2-HaloTag-PS fusion (cytosolic tandem)DepositorInsertAkt2(K14A/R25E)-Halo-PS (AKT2 )
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 8, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1852-5 pHR: U6-SpsgCD95-3 CMV-EGFP
Plasmid#84253PurposeExpresses Sp sgCD95-3 gRNA with an EGFP fluorescent markerDepositorInsertSp sgCD95-3
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSLQ1852-3 pHR: U6-SpsgCD95-2 CMV-EGFP
Plasmid#84252PurposeExpresses Sp sgCD95-2 gRNA with an EGFP fluorescent markerDepositorInsertSp sgCD95-2
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermouse U6Available sinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pES006 (pTet-qacR 2/pQacA-deGFP)
Plasmid#69035Purposereporter with qacR 2 on single plasmidDepositorInsertsUseTagsHis6 tagExpressionBacterialMutationE57Q, E58L, W61Y, E90Q, I99Q, M116Q, L119Y, E120Q…PromoterpQacA and pTetAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pES007 (pTet-qacR 5/pQacA-deGFP)
Plasmid#69036Purposereporter with qacR 5 on single plasmidDepositorInsertsUseTagsHis6 tagExpressionBacterialMutationW61Y, E90Q, F102S, M116Q, Y119L, E120QPromoterpQacA and pTetAvailable sinceSept. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X7
Plasmid#59231PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt5-ex3-5
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceDec. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X8
Plasmid#59232PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt5-ex7
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X5
Plasmid#59229PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt5 ex1
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X4
Plasmid#59228PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt4-ex7-9 and 3' UTR
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X6
Plasmid#59230PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertkrt5 ex2
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-Enh eGFP Reporter-muKC2-X3
Plasmid#59227PurposeLentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the exonic region of the keratin type II cluster.DepositorInsertKrt4-ex7-9
UseLentiviralTagsExpressionMutationPromoterMu Hsp68 minimal promoterAvailable sinceOct. 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
Px330-EGFP-LRRK2-CRISPR/Cas9
Plasmid#180437PurposePlasmid to express Cas9 from S.pyogenesand CRISPR gRNA to introduce LRRK2-G2019S mutation in human cellsDepositorInsertgRNA targeting LRRK2-G2019S (LRRK2 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pLV-CMV-LoxP-DsRed-LoxP-eGFP
Plasmid#65726PurposeFunctions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.DepositorInsertLoxP-DsRed-LoxP-eGFP
UseCre/Lox and LentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLV UBC hTMEM115-3C-3xHA-P2A-eGFP
Plasmid#214996PurposeStably express the GolgiTag (GolgiIP handle) in mammalian cells (human ORF). eGFP for cell sorting desired expression levels.DepositorInsertTMEM115 (TMEM115 Human)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterUBCAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)
Plasmid#185911PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJRH-1328 LV EF1a-CD20 IRES-EGFP
Plasmid#201918Purpose2nd generation lentiviral transfer plasmid for engineering cells to express human CD20DepositorInsertCD20
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpG-P2A-EGFP (KAC1160)
Plasmid#185916PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpG-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-StrepTagII-ProtC-FLAG
Plasmid#162090PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to StrepTagII-ProtC-FLAG
UseLentiviralTagsStrepTagII-ProtC-FLAGExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEpicVector3G_Puro-T2A-EGFP-ProtC-FLAG-AU1
Plasmid#162097PurposeEGFP linked to 3 tags on the C-terminus as barcodes. Puromycin resistant.DepositorInsertPuromycin resistant; EGFP linked to ProtC-FLAG-AU1
UseLentiviralTagsProtC-FLAG-AU1ExpressionMutationWTPromoterEF1AAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only