We narrowed to 13,007 results for: cdna

1. XE255 pCDNA3.1(zeo)-hDsh3-truncPDZ

   Plasmid

   #16754

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-hDsh3-trunc before PDZ (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncated before PDZ

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

   Plasmid

   #123342

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-EKAR-EV
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA1 3XFlag ATXN1[15Q]-delAXH

   Plasmid

   #33241

   Depositor

   Huda Zoghbi

   Article

   Lam et al Cell. 2006 Dec 29;127(7):1335-47.

   Insert

   ATXN1[2Q]-delAXH (ATXN1 Human)
   

   Use

   Tags

   3xflag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1 myc-mβTrCP F-box (1-247)

   Plasmid

   #62976

   Purpose

   Expresses myc-tagged mouse βTrCP residues 1-247

   Depositor

   Michael Ruppert

   Article

   Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

   Insert

   BTRC (Btrc Mouse)
   

   Use

   Tags

   3x myc

   Expression

   Mammalian

   Mutation

   codes for residues 1-247

   Promoter

   Available since

   May 28, 2015

   Availability

   Academic Institutions and Nonprofits only

5. 3158 pSFFV-neo Bad cDNA

   Plasmid

   #8753

   Depositor

   Stanley Korsmeyer

   Article

   Yang et al Cell 1995 Jan 27;80(2):285-91.

   Insert

   BAD (Bad Mouse)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 6, 2005

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA5-FRT-TO-Nsp7-Nsp8-HF_GC3opt

   Plasmid

   #157693

   Purpose

   mammalian expression of C-terminally His8-Flag tagged Nsp7-Nsp8 fusion protein under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Nsp7-Nsp8-HF_GC3opt (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   His8-Flag

   Expression

   Mammalian

   Mutation

   codon optimized; gene expression was optimized by…

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. XE253 pCDNA3.1(zeo)-hDsh3-82-716

   Plasmid

   #16756

   Depositor

   Randall Moon

   Article

   Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

   Insert

   FLAG-82-716-Dsh3 (DVL3 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   aa 82-716

   Promoter

   Available since

   March 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-FLAG-SBP-STAG2 (S97X)

   Plasmid

   #73964

   Purpose

   STAG2 cDNA (CCDS43990) S97X

   Depositor

   Todd Waldman

   Article

   Kim et al PLoS Genet. 2016 Feb 12;12(2):e1005865.

   Insert

   STAG2 (STAG2 Human)
   

   Use

   Tags

   FLAG-SBP

   Expression

   Mammalian

   Mutation

   S97X

   Promoter

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pCDNA3 Flag MFF iso5 S172A

   Plasmid

   #74382

   Purpose

   Flag tagged human S172A MFF isoform 5 mutant

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   Flag-MFF (MFF Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Ser 172 Ala (numbering based on MFF isoform 1)

   Promoter

   Available since

   April 13, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCDNA3-IgL-C99-T4L-rTA

    Plasmid

    #177840

    Purpose

    Tango assay

    Depositor

    Karsten Melcher

    Article

    Yan et al Acta Pharmacol Sin. 2017 Oct;38(10):1412

    Insert

    C terminal 99 amino acid fragment of human Alzheimer Amyloid beta precursor Protein (APP Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. (81) pcDNA3.1-BC2-Nup62-Flag-EPEA

    Plasmid

    #168094

    Purpose

    Nucleoporin Nup62 with N-terminal BC2 nanobody tag and C-terminal Flag and EPEA nanobody tags

    Depositor

    Christina Woo

    Article

    Ge et al Nat Chem Biol. 2021 Mar 8. pii: 10.1038/

    Insert

    Nup62-Ubc-Flag-EPEA
    

    Use

    Tags

    BC2-Tag, EPEA-tag, and Flag-tag

    Expression

    Mammalian

    Mutation

    P229A, S283T on Nup62 native sequence

    Promoter

    T7/CMV

    Available since

    May 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1-MCS-BirA(R118G)-HA-MACROD1

    Plasmid

    #172581

    Purpose

    For transient expression of C-terminal BirA-tagged MACROD1

    Depositor

    Karla Feijs

    Article

    Zaja et al Sci Rep. 2020 May 19;10(1):8286. doi: 10

    Insert

    MACROD1 (MACROD1 Human)
    

    Use

    Tags

    BirA(R118G)-HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCDNA3 Flag MFF iso5 S155,172A

    Plasmid

    #74383

    Purpose

    Flag tagged human S155,172A MFF isoform 5 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    See 155 Ala, Ser 172 Ala (numbering based on MFF …

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

14. pcDNA3.1-CAG-NanoLuc-VChR1-EYFP

    Plasmid

    #114112

    Purpose

    fusion protein of NanoLuc, Volvox channelrhodopsin-1, and enhanced yellow fluorescent protein for bioluminescent optogenetics

    Depositor

    Ute Hochgeschwender

    Article

    Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

    Insert

    NanoLuc; Volvox channelrhodopsin 1; EYFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Aug. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1(+)/AUG-stop-nLuc-3XFLAG

    Plasmid

    #127308

    Purpose

    Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    nanoLuciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA4-Tet-on-vsv-Incenp-EGFP

    Plasmid

    #108483

    Purpose

    inducible expression of vsv-INCENP wt-EGFP

    Depositor

    Susanne Lens

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. FLAG-OTUB1 T134R,C91S-pcDNA3.1

    Plasmid

    #118212

    Purpose

    Expresses FLAG-OTUB1 T134R,C91S in mammalian cells

    Depositor

    Cynthia Wolberger

    Article

    Pasupala et al J Biol Chem. 2018 Nov 23;293(47):18285-1

    Insert

    OTUB1 (OTUB1 Human)
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    changed cysteine 91 to serine and threonine 134 t…

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. YFP(1-158)-gamma-2 in pcDNAI/Amp

    Plasmid

    #55762

    Purpose

    An amino-terminal YFP fragment was fused to Ggamma2. When co-expressed with a carboxyl terminal YFP or CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.

    Depositor

    Catherine Berlot

    Article

    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep

    Insert

    YFP(1-158)-gamma-2 (GNG2 Human, Aequorea victoria)
    

    Use

    Tags

    YFP(1-158) was fused to the amino terminus of Gga…

    Expression

    Mammalian

    Mutation

    YFP (1-158) includes a substitution of Met for Gl…

    Promoter

    CMV

    Available since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-FLAG-Fos DD T325A T331A

    Plasmid

    #8973

    Depositor

    John Blenis

    Article

    Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

    Insert

    c-Fos (Fos Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S362D, S374D, T325A, T331A

    Promoter

    Available since

    Sept. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

20. pcDNA3-MAVS-M1A-invitro(KaganE12)

    Plasmid

    #52016

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation M1A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M1A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 1 to Ala

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pcDNA3-MAVS-G67M-invitro(KaganD100)

    Plasmid

    #52014

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation G67M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Gly 67 to Met

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

22. pcDNA3-MAVS-L62M-invitro(KaganD98)

    Plasmid

    #52013

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation L62M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Leu 62 to Met

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

23. pcDNA3-MAVS-E80M-invitro(KaganD99)

    Plasmid

    #52015

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation E80M

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed Glu 80 to Met

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA3-MAVS-M142A-invitro(E13)

    Plasmid

    #52017

    Purpose

    Used for in vitro expression of the human MAVS CDS containing a point mutation M142A

    Depositor

    Jonathan Kagan

    Article

    Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

    Insert

    MAVS-M142A
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Met 142 to Ala

    Promoter

    Available since

    March 24, 2014

    Availability

    Academic Institutions and Nonprofits only

25. 830 pcDNA3 GFP PTEN 1-336

    Plasmid

    #10927

    Depositor

    William Sellers

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Amino acids 1-336

    Promoter

    Available since

    Jan. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

26. pcDNA 3.2/V5-DEST CDC42EP1 S192A

    Plasmid

    #69820

    Purpose

    Expresses CDC42EP1-V5 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    CDC42 effector protein (CDC42EP1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    S192A

    Promoter

    CMV

    Available since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Nano-lantern(Ca2+)_63/pcDNA3

    Plasmid

    #51986

    Purpose

    mammalian expression of a Nano-lantern-based luminescent Ca2+ indicator with Kd=63nM

    Depositor

    Takeharu Nagai

    Article

    Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

    Insert

    Nano-lantern_CaM_E104Q-4GS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S257G in Rluc8

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

28. pcDNA4/HisMaxB-YAP2-1st WW mutant

    Plasmid

    #18998

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Tryptophan 199 changed to Alanine, and Proline 2…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

29. integrin alpha9 / alpha5 pcDNA1 neo

    Plasmid

    #13588

    Depositor

    Dean Sheppard

    Article

    Young et al Mol Biol Cell. 2001 Oct . 12(10):3214-25

    Insert

    integrin alpha 9 / alpha 5 (ITGA9 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Integrin alpha 9 chimera containing the cytoplasm…

    Promoter

    Available since

    Jan. 8, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5-FRT-TO-vsv-Incenp wt-GFP

    Plasmid

    #108502

    Purpose

    inducible expression of vsv-INCENP wt-EGFP

    Depositor

    Susanne Lens

    Article

    van der Horst et al Cell Rep. 2015 Jul 21;12(3):380-7. doi:

    Insert

    INCENP (INCENP Human)
    

    Use

    Tags

    EGFP and VSV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. Cry2 mCherry RGS4(del 1-33) in pcDNA3.1

    Plasmid

    #64207

    Purpose

    mCherry fused between Cry2 and RGS4(del 1-33) to study cell migration

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Mol Biol Cell. 2014 Aug 1;25(15):2305-14

    Inserts

    Cry2 (CRY2 Mustard Weed)
    RGS4(del 1-33) (RGS4 Human)
    

    Use

    Tags

    mcherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

32. XE170 mouse Wnt-1-myc-pcDNA3

    Plasmid

    #16854

    Depositor

    Randall Moon

    Insert

    Wnt1 (Wnt1 Mouse)
    

    Use

    Xenopus expression

    Tags

    myc (internal)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 5, 2012

    Availability

    Academic Institutions and Nonprofits only

33. Kv7.4 (1-645, Δ368-523)/pcDNA3.1

    Plasmid

    #111456

    Purpose

    Electrophysiology in HEK cell

    Depositor

    Dan Minor

    Article

    Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

    Insert

    potassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Kv7.4 (1-645, Δ368-523), C643A

    Promoter

    Available since

    May 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pcDNA3 Flag MFF iso1 S172A

    Plasmid

    #74391

    Purpose

    Flag tagged human S172A MFF isoform 1 mutant

    Depositor

    Reuben Shaw

    Article

    Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

    Insert

    Flag-MFF (MFF Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    delta exon 1 (1-26) and Ser 172 Ala.

    Promoter

    Available since

    April 13, 2016

    Availability

    Academic Institutions and Nonprofits only

35. pcDNA4/HisMaxB-YAP2-2nd WW mutant

    Plasmid

    #18999

    Depositor

    Marius Sudol

    Article

    Oka et al J Biol Chem. 2008 Jul 17. ():.

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Tryptophan 258 changed to Alanine, and Proline 2…

    Promoter

    Available since

    Sept. 8, 2008

    Availability

    Academic Institutions and Nonprofits only

36. pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

    Plasmid

    #123341

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA4/TO/myc-His-A-YAP2-deltaC

    Plasmid

    #21125

    Depositor

    Marius Sudol

    Article

    Tsutomu Oka & Marius et al

    Insert

    Yes-kinase associated protein (YAP1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    5 Carboxy-terminal amino acids -FLTWL are deleted

    Promoter

    Available since

    July 2, 2009

    Availability

    Academic Institutions and Nonprofits only

38. pcDNA3-mCherry-PKAsub-RLuc8N-NES

    Plasmid

    #138211

    Purpose

    Encodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.

    Depositor

    Jin Zhang

    Article

    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-

    Insert

    mCherry-PKAsub-RLuc8N-NES
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pcDNA 3.1 CMV NAPA-N BGH

    Plasmid

    #92278

    Purpose

    FRET probe; extracellular synapse targeted mCherry

    Depositor

    Baljit Khakh

    Article

    Octeau et al Neuron. 2018 Apr 4;98(1):49-66.e9. doi:

    Insert

    Neuron-astrocyte proximity assay - Neuronal
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. XE256 pCDNA3.1(zeo)-hDsh3-deltaCTER

    Plasmid

    #16753

    Depositor

    Randall Moon

    Article

    Angers et al Nat Cell Biol. 2006 Apr . 8(4):348-57.

    Insert

    FLAG-hDsh3-deltaCTER (DVL3 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    delta CTER

    Promoter

    Available since

    March 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge