Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

In Category

Filter By

We narrowed to 9,660 results for: Gnas

Showing: 7451 - 7500 of 9660 results

  6. pENeGFP PCNAL2 pc653

    Plasmid
    #167564
    Purpose
    The GFP-PCNAL2 fusion protein contains a SV40 nuclear localization signal at the NH2 terminus followed by an enhanced mutant GFP gene that is fused to the human PCNA by a linker.
    Depositor
    Cristina Cardoso
    Article
    Leonhardt et al J Cell Biol. 2000 Apr 17;149(2):271-80.
    Insert
    Proliferating Cell Nuclear Antigen (PCNA Human)
    Use
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    March 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    167564-plasmid-map-sequence-id-388295

    Enlarge

  15. nGR2175-gs10-NLS-VPR

    Plasmid
    #188264
    Purpose
    Plasmid ensures constitutive expression of the N-terminal fragment of split GR2 (aa 1-175), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus
    Depositor
    Roman Jerala
    Article
    Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038
    Insert
    nGR2175
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Oct. 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    188264-plasmid-map-sequence-id-374534

    Enlarge

  33. Myog'-2A-mCherryNLS-PGK-Purodtk

    Plasmid
    #69547
    Purpose
    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.
    Depositor
    Charles Gersbach
    Inserts
    2A-mCherry-NLS
    Myogenin 5' homology arm (Myog Mouse)
    Myogenin 3' homology arm (Myog Mouse)
    Puro-delta-tk
    Use
    Mouse Targeting
    Tags
    2x nuclear localization signal and PVT1-2A "…
    Expression
    Mutation
    Silent V205V mutation in myogenin gene to prevent…
    Promoter
    Available since
    April 12, 2019
    Availability
    Academic Institutions and Nonprofits only
    69547-plasmid-map-sequence-id-122991

    Enlarge

  41. EPHA7 (3NRU)

    Plasmid
    #28126
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    EPHA7:SDC173-D03:C209131 (EPHA7 Human)
    Use
    Tags
    His and Honeybee melittin Signal sequence (MKFLVN…
    Expression
    Bacterial
    Mutation
    contains amino acids residues 32-204.
    Promoter
    Available since
    Feb. 10, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  42. pCoofy62

    Plasmid
    #122014
    Purpose
    Mammalian vector for parallel SLIC cloning containing the VEGF signal sequence for secretion, N-terminal His6-Smt3Star (SumoStar*) and different C-tag options
    Depositor
    Sabine Suppmann
    Article
    Scholz et al BMC Biotechnol. 2013 Feb 14;13(1):12.
    Type
    Empty backbone
    Use
    Tags
    His6-Smt3Star and Multi3: depending on the LP2 pr…
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    May 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    122014-plasmid-map-sequence-id-243584

    Enlarge

  43. pEGFP-(RTRG)6

    Plasmid
    #69849
    Purpose
    The RTRG repeat act as a nuclear/cytoplasmic partition signal in the expressed fusion protein providing increased nuclear to cytoplasmic partition ratio when increasing the number of repeats
    Depositor
    Daniel Christophe
    Article
    Christophe-Hobertus et al Protein Pept Lett. 2008;15(4):397-401.
    Insert
    6 RTRG repeats
    Use
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Oct. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    69849-plasmid-map-sequence-id-125695

    Enlarge

  44. pET28a-λN-βLac

    Plasmid
    #162039
    Purpose
    Expresion of the fusion protein of the λN domain of the λ phage and ampR gene (encoding the enzyme β-lactamase without the native signal sequence)
    Depositor
    Karen Polizzi
    Article
    Moya Ramirez et al Biosens Bioelectron. 2019 Jul 15;137:199
    Insert
    λN domain of the λ phage and a β-lactamase fusion protein
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    162039-plasmid-map-sequence-id-325526

    Enlarge

  48. nucHCB2

    Plasmid
    #98581
    Purpose
    Nuclear Huntingtin Chromobody 2: "iVHH4" intrabody recognizing amino acids 49–148 of huntingtin, fused to eYFP and nuclear localization signal.
    Depositor
    Ray Truant
    Article
    Maiuri et al Hum Mol Genet. 2016 Dec 25. pii: ddw395.
    Insert
    HCB2
    Use
    Tags
    NLS and YFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    98581-plasmid-map-sequence-id-189303

    Enlarge

Showing: 7451 - 7500 of 9660 results