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PB-TAC-OKMS

Plasmid

#80480

Purpose

piggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry)

Depositor

Knut Woltjen

Article

Kim et al Stem Cell Reports. 2015 Apr 14;4(4):727-

Insert

OKMS (Pou5f1, Klf4, Myc, Sox2 Mouse)

Use

Piggybac transposon

Expression

Mammalian

Mutation

Klf4 [10-483]

Promoter

tetO

Available Since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pORANGE Gria3-GFP KI

Plasmid

#131491

Purpose

Endogenous tagging of GluA3: C-terminal (amino acid position: STOP codon)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Gria3, Gria3 Mouse, Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Plagl2

Plasmid

#228061

Purpose

transiently express mouse Plagl2

Depositor

Kenneth Chen

Article

Fraire et al bioRxiv [Preprint]. 2024 Apr 30:2024.04.

Insert

Plagl2 (Plagl2 Mouse)

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pDB-His-MBP-mGSDMD

Plasmid

#123365

Purpose

Expression of full-length mouse GSDMD in E. coli

Depositor

Hao Wu

Article

Orning et al Science. 2018 Nov 30;362(6418):1064-1069

Insert

GSDMD (Gsdmd Mouse)

Tags

His6-MBP

Expression

Bacterial

Promoter

T7

Available Since

May 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-tetO-Ets2

Plasmid

#70272

Purpose

Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Ets2

Depositor

Hubert Schorle

Article

Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

Insert

Ets2 (Ets2 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

tetO

Available Since

Nov. 16, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+MT-mSin3A

Plasmid

#30452

Depositor

Bob Eisenman

Article

Wotton et al Cell Growth Differ. 2001 Sep . 12(9):457

Insert

Sin3A (Sin3a Mouse)

Tags

6XMyc

Expression

Mammalian

Available Since

June 6, 2011

Availability

Academic Institutions and Nonprofits only
TET-pLKO.1 PURO shSMAD4 #1

Plasmid

#83089

Purpose

Lentiviral shRNA vector for inducible knockdown of human SMAD4 (cross reacts with mouse Smad4)

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shSMAD4 (SMAD4, Smad4 Mouse, Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pIRESh-GFPII-S-Dlk1

Plasmid

#108931

Purpose

GFP-tagged vector expressing secreted isoform of Dlk1

Depositor

Anne Ferguson-Smith

Article

Ferron et al Nature. 2011 Jul 20;475(7356):381-5. doi

Insert

Dlk1 (Dlk1 Mouse)

Tags

GFPII

Expression

Mammalian

Mutation

contains sequences from full length Dlk1A up to t…

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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TMEM16A-I546K-peGFP-N1

Plasmid

#228582

Purpose

Expresses mouse TMEM16A-I546K-eGFP in mammalian cells

Depositor

Huanghe Yang

Article

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

TMEM16A/anoctamin 1 (Ano1), transcript variant 2 (Ano1 Mouse)

Expression

Mammalian

Mutation

I546K

Available Since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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RhoA

Plasmid

#55699

Purpose

expression of mouse RhoA

Depositor

Julian Downward

Article

Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

Insert

RhoA (Rhoa Mouse)

Tags

GST

Expression

Bacterial

Promoter

lac

Available Since

Sept. 30, 2014

Availability

Academic Institutions and Nonprofits only

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pCW57.1-Neurod1-3xT7

Plasmid

#200890

Purpose

Inducible lentiviral expression of Neurod1

Depositor

Kai Ge

Article

Xie et al Nat Genet. 2023 Apr 3. doi: 10.1038/s415

Insert

Neurod1 (Neurod1 Mouse)

Use

Lentiviral; Doxycycline inducible

Tags

3xT7

Expression

Mammalian

Promoter

TRE promoter, Tet ON

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pZDonor rosa26 LSL-mMALAT1

Plasmid

#140691

Purpose

generate mouse MALAT1 conditional transgenic mice using CRISPR system targeting mouse Rosa 26 region

Depositor

Jianjun Zhao

Insert

mouse MALAT1 (Malat1 Mouse)

Use

Mouse Targeting

Available Since

Sept. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-myr-AKT-HA

Plasmid

#31790

Depositor

Xin Chen

Article

Calvisi et al Gastroenterology. 2011 Mar;140(3):1071-8

Insert

Akt1 (Akt1 Mouse)

Use

Entry vector

Tags

HA and Myr

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only
pKin1B

Plasmid

#31604

Depositor

Anthony Brown

Article

Uchida et al Mol Biol Cell. 2009 Dec . 20(23):4997-50

Insert

kinesin-1B (Kif5b Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pPB_TRE3G_scFV-sfGFP-Ring1bCD-CatMut

Plasmid

#235583

Purpose

Dox-inducible expression of control catalytic-mutant Ring1b CD fused with scFV

Depositor

Jamie Hackett

Article

Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

Insert

Ring1b (Rnf2 Mouse)

Use

CRISPR

Mutation

I53S

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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Lenti-sgCrebbp#1/Cre

Plasmid

#193209

Purpose

Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Crebbp gene

Depositor

Julien Sage

Article

Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

Insert

sgCrebbp#1 (Crebbp Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3- myc NES-mCE(K294A) NLS

Plasmid

#82467

Purpose

Expresses cytoplasmic restricted form of myc tagged mRNA capping enzyme

Depositor

Daniel Schoenberg

Article

Otsuka et al Mol Cell Biol. 2009 Apr;29(8):2155-67. d

Insert

NLS deleted from mouse Capping Enzyme (Rngtt Mouse)

Expression

Mammalian

Mutation

deletion of KRKY from 573-657

Promoter

CMV

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE

Plasmid

#67291

Purpose

Induce human iN conversion from fibroblast

Depositor

Malin Parmar

Article

Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

Insert

Asc1 (Ascl1 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

PGK

Available Since

Aug. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-3xHA-mASL

Plasmid

#34577

Purpose

NOTE: This plasmid contains 1xHA-mASL, not 3xHA-mASL.

Depositor

Peter Murray

Insert

ASL (Asl Mouse)

Tags

1xHA

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pMXs-ms-Sox7

Plasmid

#50780

Purpose

retroviral expression of mouse Sox7

Depositor

Shinya Yamanaka

Article

Nakagawa et al Nat Biotechnol. 2008 Jan;26(1):101-6. Ep

Insert

Sox7 (Sox7 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

Feb. 28, 2014

Availability

Academic Institutions and Nonprofits only

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