We narrowed to 20,285 results for: ACE;

1. pUC-hU6-2O-crScaffold_EF1a-BFP

   Plasmid

   #224862

   Purpose

   crRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoter

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Type

   Empty backbone

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6-2xTetO

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUt-MONARCH 2.0_crEGFP

   Plasmid

   #224793

   Purpose

   LPUtopia matching RMCE donor plasmid with MONARCH 2.0 circuit with crRNA targeting EGFP.

   Depositor

   Gabor Balazsi

   Article

   Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

   Inserts

   crEGFP
   Triplex stablier
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV-d2i

   Available Since

   Oct. 31, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pACYCDuet-1:ChrA-C63S

   Plasmid

   #225080

   Purpose

   Expresses precursor peptide ChrA-C63S in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrA-C63S
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   C63S

   Promoter

   T7

   Available Since

   Oct. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a:ChrH

   Plasmid

   #225084

   Purpose

   Expresses multinuclear non-heme iron dependent oxidative enzyme ChrH in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrH
   

   Use

   Tags

   His6-Thrombin

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a:ChrI

   Plasmid

   #225085

   Purpose

   Expresses anthrone-oxygenase like enzyme ChrI in E.coli

   Depositor

   Wilfred van der Donk

   Article

   Ayikpoe et al ACS Cent Sci. 2023 Apr 24;9(5):1008-1018

   Insert

   ChrI
   

   Use

   Tags

   His6 and His6-Thrombin

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   T7

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV_Myc-CylM-P2A-NLS-FLAG-YFast-TEV-His-CylLL-S15T

   Plasmid

   #225062

   Purpose

   Expression and Nuclear-localization of CylM-modified CylLL-S15T in human cells

   Depositor

   Wilfred van der Donk

   Article

   Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

   Insert

   CylLL-S15T
   

   Use

   Tags

   Multiple tags due to polycistronic expression

   Expression

   Mammalian

   Mutation

   S15T

   Promoter

   CMV

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_Myc-CylM-P2A-FLAG-YFast-TEV-His-CylLL-S15T

   Plasmid

   #225063

   Purpose

   Expression of CylM-modified CylLL-S15T in human cells

   Depositor

   Wilfred van der Donk

   Article

   Eslami et al ACS Synth Biol. 2024 Jul 19;13(7):2128-2

   Insert

   CylLL-S15T
   

   Use

   Tags

   Multiple tags due to polycistronic expression

   Expression

   Mammalian

   Mutation

   S15T

   Promoter

   CMV

   Available Since

   Oct. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBa.PEX 1-42-tdTM-FKBP

   Plasmid

   #224415

   Purpose

   Mammalian expression of Pex3

   Depositor

   Marvin Bentley

   Article

   Mendoza et al Mol Biol Cell. 2024 Jun 1;35(6):ar81. do

   Insert

   Pex3 (Pex3 Mouse)
   

   Use

   Tags

   tdTom-FKBP

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken Beta actin

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLPP4

   Plasmid

   #209966

   Purpose

   Contains Level 0 Part: constitutive Promoter (P40) for the construction of Level 1 plasmids

   Depositor

   Thorsten Mascher

   Article

   Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

   Insert

   Promoter (P40)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 13, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. pLP-EV

    Plasmid

    #209973

    Purpose

    Contains Level 0 Part: mock coding sequence (EV) for the construction of Level 1 plasmids

    Depositor

    Thorsten Mascher

    Article

    Li et al ACS Synth Biol. 2024 Sep 20;13(9):2764-2

    Insert

    CDS (mock)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Sept. 13, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

11. SL3-MS2-gRNA GG backbone (MOBE3-4)

    Plasmid

    #219949

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with SL3-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-SL3 (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3'-com-gRNA GG backbone (MOBE1-4)

    Plasmid

    #219947

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-com

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-com-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. 3'MS2-gRNA GG backbone (MOBE1-2)

    Plasmid

    #219948

    Purpose

    Golden gate backbone for mammalian expression of Sp-gRNA with 3'-MS2

    Depositor

    Alexis Komor

    Article

    Cowan et al Nat Biotechnol. 2024 May 21. doi: 10.103

    Insert

    gRNA-MS2-3'end (S. pyogenes Cas9)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pJBL7084

    Plasmid

    #167248

    Purpose

    overexpression of LacI

    Depositor

    Julius Lucks

    Article

    Jung et al ACS Synth Biol. 2023 Oct 20;12(10):2909-

    Insert

    pT7-LacI
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEVOL-tac-EcTyrRS-VSMA*-lpp-tRNA_CUA^EcTyr

    Plasmid

    #218766

    Purpose

    encodes EcTyrRS-VSMA* and EcTyr-tRNA-TAG

    Depositor

    Abhishek Chatterjee

    Article

    Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

    Insert

    EcTyrRS-VSMA*
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y37V, D167G, D182S, F183M, L186A, D265R

    Promoter

    tacI

    Available Since

    June 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. AAV hSyn mRuby

    Plasmid

    #220912

    Purpose

    Neuronal specific expression of mRuby

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Insert

    mRuby
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hSyn

    Available Since

    June 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBa.Halo-KIF1A tail

    Plasmid

    #218800

    Purpose

    Mammalian expression of rat Kif 1A

    Depositor

    Marvin Bentley

    Article

    Montgomery et al Mol Biol Cell. 2024 Mar 6:mbcE23100401.

    Insert

    Kif 1A (Kif1a Rat)
    

    Use

    Tags

    Halo

    Expression

    Mammalian

    Mutation

    Promoter

    Chicken Beta actin

    Available Since

    May 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. HA-MBP-TEVpCS-ADGRF3 CTF-FLAG

    Plasmid

    #217696

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRF3 (ADGRF3 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-752

    Promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HA-MBP-TEVpCS-ADGRE5 CTF-FLAG

    Plasmid

    #217693

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRE5 (ADGRE5 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Deletion of residues 1-530

    Promoter

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MBP-TEVpCS-ADGRA1-FLAG

    Plasmid

    #217678

    Purpose

    Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

    Depositor

    Stephen Blacklow

    Article

    Dates et al bioRxiv 2022.07.14.500097

    Insert

    ADGRA1 (ADGRA1 Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTSAR3.6t

    Plasmid

    #210258

    Purpose

    Fluorescent reporter for Type III secretion system activity in Shigella flexneri

    Depositor

    François-Xavier Campbell-Valois

    Article

    Manigat et al ACS Synth Biol. 2024 Jan 18. doi: 10.102

    Inserts

    mScarlet
    superfolder GFP
    

    Use

    Tags

    ssra

    Expression

    Bacterial

    Mutation

    Promoter

    ipaH7.8p and rpsM

    Available Since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pTcI38-PRM-wasabi

    Plasmid

    #195554

    Purpose

    Temperature-dependent expression of mWasabi reporter with transcription activation by TcI38

    Depositor

    Mikhail Shapiro

    Article

    Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

    Inserts

    mWasabi
    TcI38
    

    Use

    Tags

    6x His and AANDENYADAS degradation tag

    Expression

    Bacterial

    Mutation

    A67T, M1V, L65S, K68R, F115L, D126G, D188G

    Promoter

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pYSD101

    Plasmid

    #212925

    Purpose

    Encodes the S. cerevisiae SUC2 (Invertase) protein (Part3b') with the Aga2 signal peptide (Part3a') assembled with pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SUC2 (SUC2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pMRMTK-clo25

    Plasmid

    #203995

    Purpose

    Cloning plasmid containing the Hro ES6 insert with a chloramphenicol resistance marker

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Hro ES6
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Hro ES6

    Available Since

    Feb. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pMRMTK-clo23

    Plasmid

    #203993

    Purpose

    Cloning plasmid containing the Hro ES4 insert with a chloramphenicol resistance marker

    Depositor

    Nathan Crook

    Article

    van Schaik et al ACS Synth Biol. 2023 Oct 20;12(10):3030-

    Insert

    Hro ES4
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Hro ES4

    Available Since

    Feb. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pYSD043

    Plasmid

    #212913

    Purpose

    Encodes the S. cerevisiae 111 amino acid SCW4 Translational Fusion Partner Sequence as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    SCW4 (SCW4 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pYSD084

    Plasmid

    #212921

    Purpose

    Encodes the HA-tagged S. cerevisiae Tip1p yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    TIP1 (TIP1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYSD140

    Plasmid

    #212927

    Purpose

    Encodes the mRuby2 fluorescent protein (Part3) without any signal peptide assembled with S. cerevisiae pTEF1 promoter, tTDH1 terminator, LEU2 selectable marker and CEN/ARS yeast origin.

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pYSD080

    Plasmid

    #212917

    Purpose

    Encodes the S. cerevisiae Aga1p yeast surface display anchor protein as a Type 3 part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA1 (AGA1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pYSD001

    Plasmid

    #212898

    Purpose

    Encodes S.cerevisiae AGA2 pre- signal peptide as a Type 3a' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    AGA2 (AGA2 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge